Results 101 to 110 of about 1,124,962 (295)
Proton Pathways in Green Fluorescence Protein [PDF]
Biophysical Journal, 2005 Proton pathways in green fluorescent protein (GFP) are more extended than previously reported. In the x-ray data of wild-type GFP, a two-step exit pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton entry pathway begins at a glutamate-lysine cluster around Glu-5, and extends all the way to the buried ...
openaire +2 more sources
Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis [PDF]
, 2004 A dynamic balance of organelle fusion and fission regulates mitochondrial morphology. During apoptosis this balance is altered, leading to an extensive fragmentation of the mitochondria.
Arnoult, Damien +5 more
core +2 more sources
Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter*
Journal of Biological Chemistry, 1998 The green fluorescent protein (GFP) is a widely used reporter in gene expression and protein localization studies. GFP is a stable protein; this property allows its accumulation and easy detection in cells.
Xianqiang L Li +7 more
semanticscholar +1 more source
Fluorescent leukotriene B4: potential applications
Journal of Lipid Research, 2005 Leukotriene B4 (LTB4) is a potent lipid mediator of inflammation that acts primarily via a seven-transmembrane-spanning, G-protein-coupled receptor denoted BLT1. Here, we describe the synthesis and characterization of fluorescent analogs of LTB4 that are
Alan Sabirsh +5 more
doaj +1 more source
PLoS ONE, 2012 Two positively charged basic amino acids, arginine and lysine, are mostly exposed to protein surface, and play important roles in protein stability by forming electrostatic interactions.
Sriram Sokalingam +3 more
semanticscholar +1 more source
Murine leukemia virus (MLV) replication monitored with fluorescent proteins [PDF]
, 2006 Background: Cancer gene therapy will benefit from vectors that are able to replicate in tumor tissue and cause a bystander effect. Replication-competent murine leukemia virus (MLV) has been described to have potential as cancer therapeutics, however, MLV
Bittner, Alexandra +3 more
core
Cre Stoplight: A Red/Green Fluorescent Reporter of Cre Recombinase Expression in Living Cells
BioTechniques, 2001 The Cre/lox system is a powerful genetic tool with which to manipulate the genome. Here, we describe the development of a simple reporter system for Cre recombinase, called the Cre Stoplight.
Yvonne S. Yang, Thomas E. Hughes
doaj +1 more source
In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots [PDF]
, 2009 Background: Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome.
Frigerio, Lorenzo +4 more
core +4 more sources
A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila
Fly, 2019 The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj +1 more source