Results 111 to 120 of about 1,124,962 (295)

Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222

open access: yesPLoS ONE, 2012
Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein. Here, we report the high resolution (1.35 Å) structure of EGFP crystallised in its untagged sequence form ...
James A. J. Arpino   +2 more
semanticscholar   +1 more source

Development of a method for screening short-lived proteins using green fluorescent protein. [PDF]

open access: yes, 2004
We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library
Coffino, Philip   +2 more
core  

Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

open access: yesMicrobial Cell Factories, 2010
Background The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. Expression of recombinant GFP in E.
Kumar Avijeet   +5 more
doaj   +1 more source

The Crystal Structure of Thermal Green Protein Q66E (TGP-E) and Yellow Thermostable Protein (YTP-E) E148D

open access: yesSynBio
Thermal green protein Q66E (TGP-E) has previously shown increased thermal stability compared to thermal green protein (TGP), a thermal stable fluorescent protein produced through consensus and surface protein engineering.
Matthew R. Anderson   +3 more
doaj   +1 more source

Polyethylenimine-Modified Multiwalled Carbon Nanotubes for Plasmid DNA Gene Delivery [PDF]

open access: yes, 2009
An efficient molecular delivery technique based on the transporting high-molecular-weight PEI 600K-modified multiwalled carbon nanotubes (PEI 600K-MWCNTs) into cell membranes is reported.
Bo-Zhang Yu, Ji-Fei Ma, Wen-Xin Li
core   +1 more source

Selective Immobilization of Fluorescent Proteins for the Fabrication of Photoactive Materials

open access: yesMolecules, 2019
The immobilization of fluorescent proteins is a key technology enabling to fabricate a new generation of photoactive materials with potential technological applications. Herein we have exploited superfolder green (sGFP) and red (RFP) fluorescent proteins
Ana I. Benítez-Mateos   +5 more
doaj   +1 more source

Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell)

open access: yesIndonesian Journal of Biotechnology, 2005
The application of fluorescent proteins as expression markers and protein fusion partners has proved immensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
doaj   +1 more source

The Effect of RNA Nucleic Acid Aptamers on the Aggregation of Green Fluorescent Protein [PDF]

open access: yes, 2017
Protein aggregation can impact the product quality of pharmaceutical drugs in terms of efficacy, safety, and immunogenicity leading to economic and technical problems for biotechnology and pharmaceutical companies.
Nguyen, Thomas
core   +1 more source

Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.

open access: yesPLoS ONE, 2011
Fluorescent proteins that can switch between distinct colors have contributed significantly to modern biomedical imaging technologies and molecular cell biology.
Qi Wang   +9 more
doaj   +1 more source

Visualizing mitochondrial FoF1-ATP synthase as the target of the immunomodulatory drug Bz-423

open access: yes, 2018
Targeting the mitochondrial enzyme FoF1-ATP synthase and modulating its catalytic activities with small molecules is a promising new approach for treatment of autoimmune diseases. The immuno-modulatory compound Bz-423 is such a drug that binds to subunit
Börsch, Michael   +2 more
core   +1 more source

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