Results 161 to 170 of about 910,722 (206)

THE GREEN FLUORESCENT PROTEIN

Annual Review of Biochemistry, 1998
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and ...
openaire   +4 more sources

Deconstructing Green Fluorescent Protein

Journal of the American Chemical Society, 2008
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding to the 11th stave of the beta-barrel that is 16 amino acids long (synthetic GFP 11), following a system developed by Waldo and co-workers (Cabantous, S ...
Kevin P, Kent, William, Childs, Steven G, Boxer   +2 more
openaire   +2 more sources

GREEN FLUORESCENT PROTEIN

Photochemistry and Photobiology, 1995
Abstract— Several bioluminescent coelenterates use a secondary fluorescent protein, the green fluorescent protein (GFP), in an energy transfer reaction to produce green light. The most studied of these proteins have been the GFPs from the jellyfish Aequorea victoria and the sea pansy Renilla reniformis.
openaire   +2 more sources

Green Fluorescent Protein

2005
BACKGROUND. The Discovery of Green Fluorescent Protein. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN. Biochemical and Physical Properties of Green Fluorescent Protein.
openaire   +2 more sources

Structure of cyclized green fluorescent protein

Acta Crystallographica Section D Biological Crystallography, 2002
Crystals of cyclic green fluorescent protein (cGFP) engineered by the previously reported split intein technology [Iwai et al. (2001), J. Biol. Chem. 276, 16548-16554] were obtained and the structure was solved using molecular replacement. Although the core of the protein can unambiguously be fitted from the first to the last residue of the genuine ...
Hofmann, A, Iwai, H, Hess, S, Pluckthun, A, Wlodawer, A   +4 more
openaire   +3 more sources

Chromophore Formation in Green Fluorescent Protein

Biochemistry, 1997
The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria forms an intrinsic chromophore through cyclization and oxidation of an internal tripeptide motif [Prasher, D. C., et al. (1992) Gene 111, 229-233; Cody, C. E., et al. (1993) Biochemistry 32, 1212-1218].
B G, Reid, G C, Flynn
openaire   +2 more sources

Green fluorescent protein

2003
Methods of distinguishing between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by other autofluorescent mols. are described which entail using plane-polarized light to illuminate a sample contg. green fluorescent protein and auto-fluorescent mols.; detecting the intensity of fluorescent light that is emitted with
openaire   +1 more source

Green Fluorescent Protein for Metastasis Research

2003
Our understanding of the cancer metastatic process has advanced considerably in recent years. However, the early stages of tumor progression and micrometastasis formation have been difficult to analyze. These studies are hampered by the inability to identify small numbers of tumor cells against a background of many host cells.
openaire   +2 more sources

Green Fluorescent Protein

Proteopedia, 2013
openaire   +1 more source

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

Nature Methods, 2013
Nathan C Shaner, Gerard G Lambert, Richard N Day   +2 more
exaly