Results 211 to 220 of about 491,462 (265)

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

FLIPs: Genetically encoded molecular biosensors for functional imaging of cell signaling by linear dichroism microscopy. [PDF]

Sci Adv
Miclea P, Nagy-Marková V, Van den Eynde R, Vandenberg W, Sakhi A, Bondar A, Myšková J, Dedecker P, Lazar J.   +8 more
europepmc   +1 more source

Transparent Inorganic–Organic Bilayer Neural Electrode Array and Integration to Miniscope System for In Vivo Calcium Imaging and Electrophysiology

Advanced Functional Materials, EarlyView.
This study presents the BioCLEAR system, a highly transparent and conductive neural electrode array composed of silver nanowires (AgNWs) and doped PEDOT:PSS, enabling neural recordings with minimal optical artifacts. When integrated with a GRIN lens, this cost‐effective neural implant allows simultaneous electrophysiological recording and GCaMP6‐based ...
Dongjun Han, Kevin Cheng, Swathy Sampath Kumar, Anil Bollimunta, Elizabeth E. Torr, Juhwan Kim, Kyoungmin Kim, Hyungjun Choi, Seongkwon Hwang, Sarah Brodnick, David Cheng, Lindsay Gray, Yei Hwan Jung, William L. Murphy, Aaron J. Suminski, Jonathan J. Nassi, Justin C. Williams, Dong‐Wook Park   +17 more
wiley   +1 more source

Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking. [PDF]

Sci Rep
Subach OM, Olumayowa F, Tang M, Qiao Y, Zhang Y, Vlaskina AV, Nikolaeva AY, Kononikhin AS, Agapova YK, Belousov A, Borshchevskiy V, Samygina VR, Perfilov MM, Mishin AS, Piatkevich KD, Subach FV.   +15 more
europepmc   +1 more source

Biomimetic Iridescent Skin: Robust Prototissues Spontaneously Assembled from Photonic Protocells

Advanced Functional Materials, EarlyView.
Uniform nanoparticles are induced to form arrays (photonic crystals) in the cores of biopolymer capsules, endowing these ‘protocells’ with structural color. These protocells are then assembled into large self‐standing objects, i.e., prototissues, with robust mechanical properties as well as iridescent optical properties.
Medha Rath, Briahnah Streeter, Satyam Srivastava, Jemma Wood, William E. Bentley, Taylor J. Woehl, Srinivasa R. Raghavan   +6 more
wiley   +1 more source

A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function. [PDF]

Nat Commun
Hong J, Lee C, Madhu G, Papoulas O, Atayeter E, Hoogerbrugge G, Pan J, Takagishi M, Manzi NI, Dickinson DJ, Horani A, Brody SL, Marcotte EM, Prakash VN, Park TJ, Wallingford JB.   +15 more
europepmc   +1 more source

Microporous Microgel Assemblies Facilitating the Recruitment and Osteogenic Differentiation of Progenitor Cells for Bone Regeneration

Advanced Functional Materials, EarlyView.
There is a significant need for biomaterials with well‐defined stability and bioactivity to support tissue regeneration. In this study, we developed a tunable microgel platform that enables the decoupling of stiffness from porosity, thereby promoting bone regeneration.
Silvia Pravato, Haishuo Guan, Bianca M. Carrara, Mira Jacobs, Matteo Monticelli, Lukas Moser, Thomas Moragues, Andrew J. deMello, Tomaso Zambelli, Martin Ehrbar   +9 more
wiley   +1 more source

Callus and endosperm green fluorescence reporter-assisted selection system in maize CRISPR/Cas9 gene editing. [PDF]

BMC Plant Biol
Ge L, Li W, Dou Y, Ma Y, Sun M, Chen X, Feng X, Li Y, Yu Q.   +8 more
europepmc   +1 more source

Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion. [PDF]

Cell Rep
Hayes MI, Ravishankar S, Shanahan JK, Fountain AJ, Ramakrishnan L, Madigan CA.   +5 more
europepmc   +1 more source

Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions. [PDF]

Sci Rep
Groseclose TM, Kober E, Zupancic J, McLelland CK, Lujan LA, Kunde YA, Mozden SC, Velappan N, Lillo AM, Solomon E, Yoder J, Waldo GS, Nguyen HB.   +12 more
europepmc   +1 more source