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Reliable use of green fluorescent protein in fluorescent pseudomonads
Journal of Microbiological Methods, 2001When fluorescent pseudomonads are cultured on standard solid media under iron limiting conditions, they produce fluorescent, pigmented iron collating agents (siderophores). Siderophores can be readily identified by strong fluorescence seen under UV/blue light.
T M, Timms-Wilson, M J, Bailey
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The conformational polymorphism of the green fluorescent protein
Molecular Biology, 2012Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel ...
Haidong, Tan +5 more
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Green fluorescent protein inspired fluorophores
Advances in Colloid and Interface Science, 2020Green fluorescence proteins (GFP) are appealing to a variety of biomedical and biotechnology applications, such as protein fusion, subcellular localizations, cell visualization, protein-protein interaction, and genetically encoded sensors. To mimic the fluorescence of GFP, various compounds, such as GFP chromophores analogs, hydrogen bond-rich proteins,
Jia, Kong +5 more
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Structure of cyclized green fluorescent protein
Acta Crystallographica Section D Biological Crystallography, 2002Crystals of cyclic green fluorescent protein (cGFP) engineered by the previously reported split intein technology [Iwai et al. (2001), J. Biol. Chem. 276, 16548-16554] were obtained and the structure was solved using molecular replacement. Although the core of the protein can unambiguously be fitted from the first to the last residue of the genuine ...
Hofmann, A +4 more
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The dark side of green fluorescent protein
New Phytologist, 2005Summary Here, severe interference of chlorophyll with green fluorescent protein (GFP) fluorescence is described for medicago (Medicago truncatula), rice (Oryza sativa) and arabidopsis (Arabidopsis thaliana). This interference disrupts the proportional relationship between GFP content and fluorescence that is intrinsic to its use as a quantitative ...
Xin, Zhou +3 more
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Charge transfer in green fluorescent protein
Photochemical & Photobiological Sciences, 2006Charge transfer reactions that contribute to the photoreactions of the wild type green fluorescent protein (GFP) do not occur in the isolated p-hydroxybenzylidene-imidazolidinone chromophore, demonstrating the role of the protein environment. The high quantum efficiency of the fluorescence photocycle that includes excited state proton transfer and the ...
Jasper J, van Thor, J Timothy, Sage
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Chromophore Formation in Green Fluorescent Protein
Biochemistry, 1997The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria forms an intrinsic chromophore through cyclization and oxidation of an internal tripeptide motif [Prasher, D. C., et al. (1992) Gene 111, 229-233; Cody, C. E., et al. (1993) Biochemistry 32, 1212-1218].
B G, Reid, G C, Flynn
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The discovery of aequorin and green fluorescent protein
Journal of Microscopy, 2005We discovered aequorin and green fluorescent protein (GFP) in 1961 from the same species of jellyfish (Shimomura et al ., 1962). Our target was a luminescent substance, aequorin, and GFP was isolated as a by-product of aequorin owing to its bright conspicuous fluorescence.
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Proteins on the move: insights gained from fluorescent protein technologies
Nature Reviews Molecular Cell Biology, 2011Atsushi Miyawaki, Miyawaki Atsushi
exaly
Fluobodies: green fluorescent single-chain Fv fusion proteins
Journal of Immunological Methods, 1999Arjen Schots, Jean Martin Van Der Wolf
exaly