Lengthened autumn growing season contributes substantially to Earth's greening. [PDF]
Tian J +6 more
europepmc +1 more source
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Holly Sue Greening Oral History Interview
Holly Sue Greening, Executive Director of the Tampa Bay Estuary Program, grew up on the east coast of Florida. Inspired as a child by ichthyologist Dr.
Greening, Holly Sue +3 more
core
From Leaves to Roots: Seasonal Dynamics of Wild Boar (<i>Sus scrofa</i>) Use of Understory Plant Resources in a Forest-Agricultural Landscape of Central Poland. [PDF]
Nasiadka P +3 more
europepmc +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Critical slowing down of semiarid vegetation resilience is amplified by intensifying heatwaves. [PDF]
Fu L +7 more
europepmc +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Pre-anthesis light signaling of sheathed developing barley inflorescences defines floral fate. [PDF]
Babanna ST +3 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Urban tree canopy fragmentation and child mortality in low- and middle-income countries. [PDF]
Chi D +14 more
europepmc +1 more source

