Results 71 to 80 of about 4,426,015 (295)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Building Deformation Prediction Based on Ground Surface Settlements of Metro-Station Deep Excavation

open access: yesAdvances in Civil Engineering, 2018
Building deformations are not only closely related to the distance from the building to metro-station excavation but also related to the relative positions of the building and metro-station excavation.
Dapeng Li, Changhong Yan
doaj   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

open access: yesFEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton   +6 more
wiley   +1 more source

The behaviour of modern flexible framed structures undergoing differential settlement

open access: yes, 2010
Modern office buildings are often open plan buildings with a frame consisting of flat RC slabs, RC columns and non-load bearing internal and external partitions and facades.
Smit, Gerrit
core   +1 more source

Calculation Model for Ground Settlement Process of Xi’an Subway Tunnel Non-Synchronous Excavation with Double-Line

open access: yes, 2011
The current calculation models of ground settlement for tunnel excavation cannot reflect ground settlement of tunnel non-synchronous excavation with double-line.
Sheng Jun Shao   +3 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Numerical Analysis of Ground Settlement of Close Buried Double-Line Shield Tunnels Under Different Excavation Conditions

open access: yesApplied Sciences
Construction-induced mutual disturbance exerts a substantial influence on the ground settlement of double-line tunnels, and the ground settlement of double-line tunnels differs under varying excavation conditions.
Zhiheng Jiang, Yuke Wang
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Monitoring and Analysis of Ground Settlement Induced by Tunnelling with Slurry Pressure-Balanced Tunnel Boring Machine

open access: yesAdvances in Civil Engineering, 2018
A case study of monitoring and analysis of ground settlement caused by tunnelling of stacked twin tunnels for underground metro line construction through the densely populated area using the slurry pressure-balanced TBM is presented.
Hyunku Park   +3 more
doaj   +1 more source

Settlement Agreement - 2012 Place Holder

open access: yes, 2012
Settlement Agreement sent to Interior April 2012Settlement Agreement sent to Interior April ...
Settlement Parties
core  

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