Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
A Practical Guide to Optimal Adaptive Two-Stage Designs for Various Endpoint Types. [PDF]
Bruder N, Meis J, Pilz M, Kieser M.
europepmc +1 more source
Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara +10 more
wiley +1 more source
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Exceptional-point stability boundaries from quantum dissipation to cosmological acceleration. [PDF]
Christensen N.
europepmc +1 more source
No cure for cure models: killed by competing risks. [PDF]
Putter H, Andersen PK.
europepmc +1 more source
Architectural good practices for reproducible benchmarking in protein machine learning. [PDF]
García-Vinuesa J +8 more
europepmc +1 more source
A journal article reporting standard working group in sport and exercise science: a call to action. [PDF]
Warneke K +4 more
europepmc +1 more source
Response to Letter to the Editor for 'Mental Health Problems in Traumatic Spinal Cord Injury Patients - An Umbrella Systematic Review'. [PDF]
Muthu S, Viswanathan VK.
europepmc +1 more source

