Results 1 to 10 of about 456,042 (161)
Functional identification of catalytic metal ion binding sites within RNA. [PDF]
PLoS Biology, 2005 The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors.
James L Hougland +3 more
doaj +15 more sources
Mechanically probing the folding pathway of single RNA molecules [PDF]
Biophys. J. 84 (2003) 2831-2840, 2002 We study theoretically the denaturation of single RNA molecules by mechanical stretching, focusing on signatures of the (un)folding pathway in molecular fluctuations. Our model describes the interactions between nucleotides by incorporating the experimentally determined free energy rules for RNA secondary structure, while exterior single stranded ...
Banerjee +24 more
arxiv +9 more sources
Computational Identification of Four Spliceosomal snRNAs from the Deep-Branching Eukaryote Giardia intestinalis [PDF]
, 2008 Funding: Marsden Fund New Zealand Allan Wilson Centre The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.RNAs processing other RNAs is very general in eukaryotes, but is not clear
David Penny +4 more
core +18 more sources
Frontiers in Plant Science, 2022 Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown ...
Kaijian Fan +9 more
doaj +1 more source
Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases. [PDF]
PLoS Biology, 2014 The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (mtTyrRS; CYT-18 protein) evolved a new function as a group I intron splicing factor by acquiring the ability to bind group I intron RNAs and stabilize their catalytically active RNA structure ...
Lilian T Lamech +2 more
doaj +1 more source
Organellar Introns in Fungi, Algae, and Plants
Cells, 2021 Introns are ubiquitous in eukaryotic genomes and have long been considered as ‘junk RNA’ but the huge energy expenditure in their transcription, removal, and degradation indicate that they may have functional significance and can offer evolutionary ...
Jigeesha Mukhopadhyay, Georg Hausner
doaj +1 more source
Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM
Nature Communications, 2023 Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 ...
Shanshan Li +4 more
doaj +1 more source
Spliceozymes: ribozymes that remove introns from pre-mRNAs in trans. [PDF]
PLoS ONE, 2014 Group I introns are pre-mRNA introns that do not require the spliceosome for their removal. Instead, they fold into complex three-dimensional structures and catalyze two transesterification reactions, thereby excising themselves and joining the flanking ...
Zhaleh N Amini +2 more
doaj +1 more source
Life, 2021 A hallmark of sea anemone mitochondrial genomes (mitogenomes) is the presence of complex catalytic group I introns. Here, we report the complete mitogenome and corresponding transcriptome of the carpet sea anemone Stichodactyla haddoni (family ...
Steinar Daae Johansen +3 more
doaj +1 more source
The mitochondrial genome of the prasinophyte Prasinoderma coloniale reveals two trans-spliced group I introns in the large subunit rRNA gene. [PDF]
PLoS ONE, 2013 Organelle genes are often interrupted by group I and or group II introns. Splicing of these mobile genetic occurs at the RNA level via serial transesterification steps catalyzed by the introns'own tertiary structures and, sometimes, with the help of ...
Jean-François Pombert +3 more
doaj +1 more source