Results 111 to 120 of about 62,692 (225)

CRISPR/Cas9‐Mediated Knockouts of the ALG3 and GNTI in N. benthamiana and Their Application to Pharmaceutical Production

Plant Biotechnology Journal, EarlyView.
ABSTRACT N‐Glycosylation critically influences the efficacy, safety and pharmacokinetic properties of biopharmaceuticals. Plant expression platforms offer multiple advantages for the production of N‐glycosylated proteins, but their use is impeded by the presence of plant‐specific N‐glycan epitopes, which raise concerns of possible immunogenicity to ...
Dolgormaa Bataa, Hiroyuki Kajiura, Reimi Lai Sang Sawada‐Choi, Yukino Yamashita, Takeshi Ishimizu, Ryo Misaki, Atsushi Takeda, Kazuhito Fujiyama   +7 more
wiley   +1 more source

Optimized catalytic DNA-cleaving ribozymes [PDF]

, 1996
The present invention discloses nucleic acid enzymes capable of cleaving nucleic acid molecules, including single-stranded DNA, in a site-specific manner under physiologic conditions, as well as compositions including same.
Joyce, Gerald F.
core   +1 more source

Reduced Susceptibility to Phytophthora in Non‐Transgenic Cacao Progeny Through CRISPR–Cas9 Mediated TcNPR3 Mutagenesis

Plant Biotechnology Journal, EarlyView.
ABSTRACT Black pod disease, caused by a complex of Phytophthora species, poses a severe threat to global cacao production. This study explores the use of CRISPR–Cas9 genome editing to reduce disease susceptibility in Theobroma cacao L. by targeting the TcNPR3 gene, a known negative regulator of plant defence.
Mark J. Guiltinan, Lena Landherr, Siela N. Maximova, Dante DelVecchio, Aswathy Sebastian, Istvan Albert   +5 more
wiley   +1 more source

Calmodulin‐Like Protein MfCML50 Interacts With Carveol Dehydrogenase in Medicago falcata to Regulate Cold Tolerance Through Mediating ROS Homeostasis

Plant Biotechnology Journal, EarlyView.
ABSTRACT Low temperature triggers Ca2+ signalling and reprogramming of gene expression and metabolism in plants. However, how the Ca2+ signal is transduced to the downstream metabolic pathways remains unknown. The involvement of a cold‐induced calmodulin‐like protein, MfCML50, from Medicago falcata in regulation of cold tolerance was examined in the ...
Bohao Geng, Shuhan Yu, Qiguo Sun, Lei Yang, Qian Yang, Hailong Yang, Zhenfei Guo   +6 more
wiley   +1 more source

The RNA m6A Methyltransferase PvFIP37 Activates Vacuole Transport and Glutathione Detoxification Pathways to Increase Switchgrass Cd Tolerance

Plant Biotechnology Journal, EarlyView.
ABSTRACT Cadmium (Cd) pollution is a growing global issue that poses significant threats to ecosystems and human health. N6‐methyladenosine (m6A) plays an essential role in regulating plant growth and stress responses, but the biological functions and regulatory mechanisms of m6A core enzymes under Cd stress remain largely unexplored. In this study, we
Mengzhuo Lin, Lei Wang, Bowen Liu, Huayue Liu, Wenwu Qian, Die Zhou, Hui Zang, Binglin Hong, Yunwei Zhang, Jishan Jiang   +9 more
wiley   +1 more source

Synthetic Biology and Metabolic Engineering of Microalgae for Sustainable Lipid and Terpenoid Production: An Updated Perspective

Plant Biotechnology Journal, EarlyView.
ABSTRACT Microalgae are increasingly recognised as powerful platforms for the sustainable production of lipids and terpenoids, with expanding applications in the food, fuel and biomanufacturing industries. In this updated review, we consolidate and critically assess the most recent advances in synthetic biology and metabolic engineering of key ...
Ty Shitanaka, Yu Wang, Sally Do, Julia Yuson, Samir Kumar Khanal, Krzysztof Zienkiewicz, Zhi‐Yan Du   +6 more
wiley   +1 more source

BolAG‐BolRPI2‐BolBAM4 Module Regulates the Bolting of Curd by Modulating Starch Metabolism in Broccoli (Brassica oleracea var. italica)

Plant Biotechnology Journal, EarlyView.
ABSTRACT The curd is a unique indeterminate inflorescence structure, which is the most important edible organ of broccoli. Premature bolting of the curd is a major factor that limits the yield and marketability of broccoli. However, the formation and development of broccoli curd, especially the bolting of curd, remain poorly understood.
Yinxia Zhu, Mengwei Xi, Yuxuan Duan, Hanmin Jiang, Jingjing Xie, Mengyao Zhao, Ce Liu, Hui Li, Chunguo Wang   +8 more
wiley   +1 more source

Transgene‐Free, Gene‐Edited Cavendish Bananas (Musa acuminata, AAA)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Global consumer acceptance of gene‐edited food crops is increasing with new breeding technologies that can modify the genome without foreign DNA integration. Here, we report an Agrobacterium‐based system for transgene‐free, gene editing of the banana cultivar, Cavendish.
Maiko Kato, Jen Kleidon, Amba Phillips, Alexandra Tabrett, Robert Harding, James Dale, Jean‐Yves Paul   +6 more
wiley   +1 more source

Polymorphisms in the WNK1 gene are asociated with blood pressure variation and urinary potassium excretion [PDF]

, 2009
WNK1 - a serine/threonine kinase involved in electrolyte homeostasis and blood pressure (BP) control - is an excellent candidate gene for essential hypertension (EH). We and others have previously reported association between WNK1 and BP variation. Using
Brown, M, Brunner, E, Burke, B, Cappucio, FP, Caulfield, M, Chambers, J, Clarke, R, Collins, R, Connell, JM, Dobson, R, Dominiczak, AF, Elliott, P, Eyheramendy, S, Farrall, M, Hoti, M, Howard, P, Iacone, R, Ji, C, Juhanson, P, Kooner, J, Kumari, M, Laan, M, Lathrop, GM, Lee, K, Marmot, M, Munroe, PB, Newhouse, S, Onipinla, A, Org, E, Samani, NJ, Shaw-Hawkins, S, Strazzullo, P, Veldre, G, Viigimaa, M, Wallace, C   +34 more
core  

The Epigenetic Regulation of Agronomic Traits and Environmental Adaptability in Brassicas

Plant, Cell &Environment, EarlyView.
ABSTRACT As essential sources of vegetables, oilseeds, and forage, Brassica crops exhibit complex epigenetic regulation mechanisms involving histone modifications, DNA modifications, RNA modifications, noncoding RNAs, and chromatin remodelling. The agronomic traits and environmental adaptability of crops are regulated by both genetic and epigenetic ...
Daolei Zhang, Yin Lu, Wei Ma, Jianjun Zhao   +3 more
wiley   +1 more source