Results 11 to 20 of about 49,487 (207)
Mechanism of 3' splice site selection by the catalytic core of the sunY intron of bacteriophage T4: the role of a novel base-pairing interaction in group I introns. [PDF]
Genes & Development, 1990 The catalytic core of the sunY intron of bacteriophage T4 is separated from its 3' exon by 837 nucleotides, most of which are part of an open reading frame (ORF). Here, we report that transcripts truncated within the sunY ORF self-splice in vitro to a variety of sites in the segment immediately 3' of the core. Recognition of these proximal splice sites
F, Michel, P, Netter, M Q, Xu, D A, Shub
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Cell, 1995 The yeast mitochondrial group I intron b15 undergoes self-splicing at high Mg2+ concentrations, but requires the splicing factor CBP2 for reaction under physiological conditions. Chemical accessibility and UV cross-linking experiments now reveal that self-processing is slow because functional elements are not properly positioned in an active tertiary ...
Weeks, Kevin M, Cech, Thomas R
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Functional identification of catalytic metal ion binding sites within RNA. [PDF]
PLoS Biology, 2005 The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors.
James L Hougland +3 more
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Frontiers in Plant Science, 2022 Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown ...
Kaijian Fan +9 more
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Cluster J Mycobacteriophages: Intron Splicing in Capsid and Tail Genes [PDF]
, 2013 Bacteriophages isolated on Mycobacterium smegmatis mc2155 represent many distinct genomes sharing little or no DNA sequence similarity. The genomes are architecturally mosaic and are replete with genes of unknown function.
Best, AA +17 more
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Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases. [PDF]
PLoS Biology, 2014 The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (mtTyrRS; CYT-18 protein) evolved a new function as a group I intron splicing factor by acquiring the ability to bind group I intron RNAs and stabilize their catalytically active RNA structure ...
Lilian T Lamech +2 more
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Organellar Introns in Fungi, Algae, and Plants
Cells, 2021 Introns are ubiquitous in eukaryotic genomes and have long been considered as ‘junk RNA’ but the huge energy expenditure in their transcription, removal, and degradation indicate that they may have functional significance and can offer evolutionary ...
Jigeesha Mukhopadhyay, Georg Hausner
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Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM
Nature Communications, 2023 Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 ...
Shanshan Li +4 more
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Spliceozymes: ribozymes that remove introns from pre-mRNAs in trans. [PDF]
PLoS ONE, 2014 Group I introns are pre-mRNA introns that do not require the spliceosome for their removal. Instead, they fold into complex three-dimensional structures and catalyze two transesterification reactions, thereby excising themselves and joining the flanking ...
Zhaleh N Amini +2 more
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Life, 2021 A hallmark of sea anemone mitochondrial genomes (mitogenomes) is the presence of complex catalytic group I introns. Here, we report the complete mitogenome and corresponding transcriptome of the carpet sea anemone Stichodactyla haddoni (family ...
Steinar Daae Johansen +3 more
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