Results 11 to 20 of about 61,070 (218)

Functional Identification of Ligands for a Catalytic Metal Ion in Group I Introns [PDF]

Biochemistry, 2008
Many enzymes use metal ions within their active sites to achieve enormous rate acceleration. Understanding how metal ions mediate catalysis requires elucidation of metal ion interactions with both the enzyme and the substrate(s). The three-dimensional arrangement determined by X-ray crystallography provides a powerful starting point for identifying ...
Marcello Forconi, Ji Hee Lee, Jungjoon K. Lee, Joseph A. Piccirilli, Daniel Herschlag   +4 more
openalex   +4 more sources

Folding of group I introns from bacteriophage T4 involves internalization of the catalytic core. [PDF]

Proceedings of the National Academy of Sciences, 1991
Fe(II)-EDTA, a solvent-based cleavage reagent that distinguishes between the inside and outside surfaces of a folded RNA molecule, has revealed some of the higher-order folding of the group IB intron from Tetrahymena thermophila pre-rRNA. This reagent has now been used to analyze the bacteriophage T4 sunY and td introns, both of which are members of ...
Timothy S. Heuer, P. Scott Chandry, Marlene Belfort, Daniel W. Celander, Thomas R. Cech   +4 more
openalex   +5 more sources

Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation

Molecules, 2016
Origins-of-life research requires searching for a plausible transition from simple chemicals to larger macromolecules that can both hold information and catalyze their own production.
Laura Elizabeth Satterwhite, Jessica A. M. Yeates, Niles Lehman   +2 more
doaj   +2 more sources

Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock

Molecules, 2016
Group I introns in nuclear ribosomal RNA of eukaryotic microorganisms are processed by splicing or circularization. The latter results in formation of full-length circular introns without ligation of the exons and has been proposed to be active in intron
Kasper L. Andersen, Bertrand Beckert, Benoit Masquida, Steinar D. Johansen, Henrik Nielsen   +4 more
doaj   +2 more sources

Molecular insights into de novo small-molecule recognition by an intron RNA structure. [PDF]

Proc Natl Acad Sci U S A
Significance The ability to target and regulate RNA function with small molecules has vast potential, but molecular understanding of the metrics governing RNA–drug interactions is limited.
Liu T, Xu L, Chung K, Sisto LJ, Hwang J, Zhang C, Van Zandt MC, Pyle AM.   +7 more
europepmc   +2 more sources

Computational Identification of Four Spliceosomal snRNAs from the Deep-Branching Eukaryote Giardia intestinalis [PDF]

, 2008
Funding: Marsden Fund New Zealand Allan Wilson Centre The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.RNAs processing other RNAs is very general in eukaryotes, but is not clear
David Penny, J. White, Lesley J. Collins, W. Timothy, Xiaowei Sylvia Chen   +4 more
core   +18 more sources

Functional identification of catalytic metal ion binding sites within RNA. [PDF]

PLoS Biology, 2005
The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors.
James L Hougland, Alexander V Kravchuk, Daniel Herschlag, Joseph A Piccirilli   +3 more
doaj   +2 more sources

ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2

Frontiers in Plant Science, 2022
Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown ...
Kaijian Fan, Qinghui Fu, Qianhan Wei, Sinian Jia, Anqi Zhao, Tengteng Wang, Jie Cao, Yan Liu, Zhenjing Ren, Yunjun Liu   +9 more
doaj   +1 more source

Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases. [PDF]

PLoS Biology, 2014
The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (mtTyrRS; CYT-18 protein) evolved a new function as a group I intron splicing factor by acquiring the ability to bind group I intron RNAs and stabilize their catalytically active RNA structure ...
Lilian T Lamech, Anna L Mallam, Alan M Lambowitz   +2 more
doaj   +1 more source

Organellar Introns in Fungi, Algae, and Plants

Cells, 2021
Introns are ubiquitous in eukaryotic genomes and have long been considered as ‘junk RNA’ but the huge energy expenditure in their transcription, removal, and degradation indicate that they may have functional significance and can offer evolutionary ...
Jigeesha Mukhopadhyay, Georg Hausner
doaj   +1 more source