Results 191 to 200 of about 62,692 (225)
The Journal of Biochemistry, 1994 Two adenosine residues, universally conserved among group I introns, are located in the L4 region of the catalytic core. Base-substitution mutations in these adenosines resulted in diminished in vitro self-splicing activity of the Tetrahymena group I intron, more severely for double than for single mutations. The defect caused by the mutation of the L4
Tan Inoue +2 more
semanticscholar +5 more sources
Journal of Molecular Biology, 1998 The aminoglycoside antibiotic neomycin B induces misreading of the genetic code during translation and inhibits several ribozymes. The self-splicing group I intron derived from the T4 phage thymidylate synthase (td) gene is one of these. Here we report how neomycin B binds to the intron RNA inhibiting splicing in vitro.
C Berens +3 more
semanticscholar +5 more sources
Recent Patents on DNA & Gene Sequences, 2010 The importance of RNA in vital cellular events like gene expression, transport, self-splicing catalytic activity etc., renders them an alternative target for drugs and other specific RNA binding ligands. RNA targets gain significance for the fact that targeting DNA with therapeutics sooner leads to drug resistance and severe side effects by impairing ...
Irudayam Maria Johnson
semanticscholar +5 more sources
Gene, 1994 The P9 stem-loop is one of the conserved structural elements found in all group-I introns. Using two deletion mutants in this region of the Tetrahymena thermophilia large ribosomal subunit intron, we show that removal of the P9 element, either alone, or together with the non-conserved downstream P9.1 and P9.2 elements, results in an intron incapable of
Mark G. Caprara, Richard B. Waring
semanticscholar +5 more sources
Phylogenetic and genetic evidence for base-triples in the catalytic domain of group I introns
Nature, 1990 Understanding the mechanisms by which ribozymes catalyse chemical reactions requires a detailed knowledge of their structure. The secondary structure of the group I introns has been confirmed by comparison of over 70 published sequences, by chemical protection studies, and by genetic experiments involving compensatory mutations.
Sandra Couture +3 more
openaire +4 more sources
Journal of Molecular Biology, 1990 Alignment of the 87 available sequences of group I self-splicing introns reveals numerous instances of covariation between distant sites. Some of these covariations cannot be ascribed to historical coincidences or the known secondary structure of group I introns, and are, therefore, best explained as reflecting tertiary contacts.
Eric Westhof, François Michel
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Current Genetics, 1999 The sequences and predicted secondary structures of the four catalytic group-I introns in the psbA gene of Chlamydomonas reinhardtii, Cr.psbA-1-Cr.psbA-4, have been determined. Cr.psbA-1 and Cr.psbA-4 are subgroup-IA1 introns and have similar secondary structures, except at the 3' end where Cr.psbA-1 contains a large inverted-repeat domain.
Nita N. Deshpande +2 more
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Cell, 1992 We have used an in vitro reconstitution system to determine the effects of a large number of mutations in the highly conserved 5' terminal domain of the yeast U2 snRNA on pre-mRNA splicing. Whereas many mutations have little or no functional consequence, base substitutions in two regions were found to have drastic effects on pre-mRNA splicing.
David S. McPheeters, John Abelson
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Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature, 2009 Sabine Müller
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