Results 21 to 30 of about 61,070 (218)

Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM

Nature Communications, 2023
Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 ...
Shanshan Li, Michael Z. Palo, Xiaojing Zhang, Grigore Pintilie, Kaiming Zhang   +4 more
doaj   +1 more source

Spliceozymes: ribozymes that remove introns from pre-mRNAs in trans. [PDF]

PLoS ONE, 2014
Group I introns are pre-mRNA introns that do not require the spliceosome for their removal. Instead, they fold into complex three-dimensional structures and catalyze two transesterification reactions, thereby excising themselves and joining the flanking ...
Zhaleh N Amini, Karen E Olson, Ulrich F Müller   +2 more
doaj   +1 more source

The Mitochondrial Genome of the Sea Anemone Stichodactyla haddoni Reveals Catalytic Introns, Insertion-Like Element, and Unexpected Phylogeny

Life, 2021
A hallmark of sea anemone mitochondrial genomes (mitogenomes) is the presence of complex catalytic group I introns. Here, we report the complete mitogenome and corresponding transcriptome of the carpet sea anemone Stichodactyla haddoni (family ...
Steinar Daae Johansen, Sylvia I. Chi, Arseny Dubin, Tor Erik Jørgensen   +3 more
doaj   +1 more source

The mitochondrial genome of the prasinophyte Prasinoderma coloniale reveals two trans-spliced group I introns in the large subunit rRNA gene. [PDF]

PLoS ONE, 2013
Organelle genes are often interrupted by group I and or group II introns. Splicing of these mobile genetic occurs at the RNA level via serial transesterification steps catalyzed by the introns'own tertiary structures and, sometimes, with the help of ...
Jean-François Pombert, Christian Otis, Monique Turmel, Claude Lemieux   +3 more
doaj   +1 more source

RNA folding and catalysis mediated by iron (II). [PDF]

PLoS ONE, 2012
Mg²⁺ shares a distinctive relationship with RNA, playing important and specific roles in the folding and function of essentially all large RNAs. Here we use theory and experiment to evaluate Fe²⁺ in the absence of free oxygen as a replacement for Mg²⁺ in
Shreyas S Athavale, Anton S Petrov, Chiaolong Hsiao, Derrick Watkins, Caitlin D Prickett, J Jared Gossett, Lively Lie, Jessica C Bowman, Eric O'Neill, Chad R Bernier, Nicholas V Hud, Roger M Wartell, Stephen C Harvey, Loren Dean Williams   +13 more
doaj   +1 more source

Template-jumping prime editing enables large insertion and exon rewriting in vivo

Nature Communications, 2023
Targeted insertion of large DNA fragments holds promise for genome engineering and gene therapy. Prime editing (PE) effectively inserts short (400 bp) remains low and in vivo application has not been demonstrated.
Chunwei Zheng, Bin Liu, Xiaolong Dong, Nicholas Gaston, Erik J. Sontheimer, Wen Xue   +5 more
doaj   +1 more source

Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design

Biology, 2017
A bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures.
Md. Motiar Rahman, Shigeyoshi Matsumura, Yoshiya Ikawa   +2 more
doaj   +1 more source

DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA. [PDF]

, 2017
Diversity-generating retroelements (DGRs) are molecular evolution machines that facilitate microbial adaptation to environmental changes. Hypervariation occurs via a mutagenic retrotransposition process from a template repeat (TR) to a variable repeat ...
Guo, Huatao, Han, Jin, Heng, Xiao, Lee, William R, Miller, Jeff F, Naorem, Santa S, Wang, Shufang   +6 more
core   +3 more sources

Structural Divergence of the Group I Intron Binding Surface in Fungal Mitochondrial Tyrosyl-tRNA Synthetases That Function in RNA Splicing*

Journal of Biological Chemistry, 2016
The mitochondrial tyrosyl-tRNA synthetases (mtTyrRSs) of Pezizomycotina fungi, a subphylum that includes many pathogenic species, are bifunctional proteins that both charge mitochondrial tRNATyr and act as splicing cofactors for autocatalytic group I ...
L. Lamech, M. Saoji, P. Paukstelis, A. Lambowitz   +3 more
semanticscholar   +1 more source

tRNA splicing [PDF]

, 1998
Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is a crucial step in gene expression. Interestingly, even within Eukarya there are at least four splicing mechanisms.
Abelson, John, Li, Hong, Trotta, Christopher R.   +2 more
core   +1 more source