Results 1 to 10 of about 364,363 (199)
Group II intron-anchored gene deletion in Clostridium. [PDF]
PLoS ONE, 2011 Clostridium plays an important role in commercial and medical use, for which targeted gene deletion is difficult. We proposed an intron-anchored gene deletion approach for Clostridium, which combines the advantage of the group II intron "ClosTron" system
Kaizhi Jia +3 more
doaj +8 more sources
Did group II intron proliferation in an endosymbiont-bearing archaeon create eukaryotes? [PDF]
Biology Direct, 2006 Martin & Koonin recently proposed that the eukaryote nucleus evolved as a quality control mechanism to prevent ribosome readthrough into introns. In their scenario, the bacterial ancestor of mitochondria was resident in an archaeal cell, and group II ...
Poole Anthony M
doaj +6 more sources
Group II intron-based gene targeting reactions in eukaryotes. [PDF]
PLoS ONE, 2008 Mobile group II introns insert site-specifically into DNA target sites by a mechanism termed retrohoming in which the excised intron RNA reverse splices into a DNA strand and is reverse transcribed by the intron-encoded protein.
Marta Mastroianni +7 more
doaj +6 more sources
Database for bacterial group II introns [PDF]
Nucleic Acids Research, 2011 The Database for Bacterial Group II Introns (http://webapps2.ucalgary.ca/~groupii/index.html#) provides a catalogue of full-length, non-redundant group II introns present in bacterial DNA sequences in GenBank.
A. Duong +22 more
core +6 more sources
Inverse splicing of a group II intron. [PDF]
Proceedings of the National Academy of Sciences, 1993 I describe the self-splicing of an RNA that consists of exon sequences flanked by group II intron sequences. I find that this RNA undergoes accurate splicing in vitro, yielding an excised exon circle. This splicing reaction involves the joining of the 5' splice site at the end of an exon to the 3' splice site at the beginning of the same exon; thus, I ...
Kevin A. Jarrell
openalex +4 more sources
Functionality of in vitro reconstituted group II intron RmInt1-derived ribonucleoprotein particles [PDF]
Frontiers in Molecular Biosciences, 2016 The functional unit of mobile group II introns is a ribonucleoprotein particle (RNP) consisting of the intron-encoded protein (IEP) and the excised intron RNA. The IEP has reverse transcriptase activity but also promotes RNA splicing, and the RNA-protein
María Dolores Molina-Sánchez +2 more
doaj +3 more sources
Structural basis for the second step of group II intron splicing [PDF]
Nature Communications, 2018 Group II introns are large, self-splicing RNAs that catalyze their own excision from pre-mRNA molecules. Here the authors determine the 3.7 Å crystal structure of the group II intron in the stage immediately before the second step of splicing and present
Russell T. Chan +5 more
doaj +4 more sources
The retrohoming of linear group II intron RNAs in Drosophila melanogaster occurs by both DNA ligase 4-dependent and -independent mechanisms. [PDF]
PLoS Genetics, 2012 Mobile group II introns are bacterial retrotransposons that are thought to have invaded early eukaryotes and evolved into introns and retroelements in higher organisms.
Travis B White, Alan M Lambowitz
doaj +7 more sources
Group II intron protein localization and insertion sites are affected by polyphosphate. [PDF]
PLoS Biology, 2008 Mobile group II introns consist of a catalytic intron RNA and an intron-encoded protein with reverse transcriptase activity, which act together in a ribonucleoprotein particle to promote DNA integration during intron mobility.
Junhua Zhao +5 more
doaj +6 more sources
Group II introns in the bacterial world [PDF]
Molecular Microbiology, 2000 Group II introns are large catalytic RNA molecules that act as mobile genetic elements. They were initially identified in the organelle genomes of lower eukaryotes and plants, and it has been suggested that they are the progenitors of nuclear spliceosomal introns.
Francisco Martı́nez-Abarca +1 more
openalex +4 more sources