Functionality of in vitro reconstituted group II intron RmInt1-derived ribonucleoprotein particles [PDF]
Frontiers in Molecular Biosciences, 2016 The functional unit of mobile group II introns is a ribonucleoprotein particle (RNP) consisting of the intron-encoded protein (IEP) and the excised intron RNA. The IEP has reverse transcriptase activity but also promotes RNA splicing, and the RNA-protein
María Dolores Molina-Sánchez +2 more
doaj +5 more sources
Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. [PDF]
PLoS Genetics, 2015 Mobile bacterial group II introns are evolutionary ancestors of spliceosomal introns and retroelements in eukaryotes. They consist of an autocatalytic intron RNA (a "ribozyme") and an intron-encoded reverse transcriptase, which function together to ...
David M Truong +4 more
doaj +5 more sources
Group II intron and repeat-rich red algal mitochondrial genomes demonstrate the dynamic recent history of autocatalytic RNAs [PDF]
BMC Biology, 2022 Background Group II introns are mobile genetic elements that can insert at specific target sequences, however, their origins are often challenging to reconstruct because of rapid sequence decay following invasion and spread into different sites.
Dongseok Kim +5 more
doaj +2 more sources
Updated Progress on Group II Intron Splicing Factors in Plant Chloroplasts [PDF]
Current Issues in Molecular Biology, 2022 Group II introns are large catalytic RNAs (ribozymes) in the bacteria and organelle genomes of several lower eukaryotes. Many critical photosynthesis-related genes in the plant chloroplast genome also contain group II introns, and their splicing is ...
Chu Zeng +3 more
doaj +2 more sources
Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome [PDF]
Frontiers in Molecular Biosciences, 2022 RmInt1 is a group II intron encoding a reverse transcriptase protein (IEP) lacking the C-terminal endonuclease domain. RmInt1 is an efficient mobile retroelement that predominantly reverse splices into the transient single-stranded DNA at the template ...
María Dolores Molina-Sánchez +3 more
doaj +2 more sources
Cryo-EM Structures of Group II Intron Ribonucleoprotein Complexes [PDF]
Structural DynamicsGroup II introns are ribozymes that catalyze self-splicing reactions through two competing pathways: a non- physiological hydrolytic reaction and a branched splicing reaction that generates intron lariats.
Sarah A Starcovic +2 more
doaj +2 more sources
A specialized bacterial group II intron is a highly efficient retrotransposon [PDF]
Mobile DNAMobile group II introns are site-specific retrotransposons composed of a large self-splicing ribozyme and an intron-encoded reverse transcriptase that are widespread in bacterial and organellar genomes.
Lucie Gomes +3 more
doaj +2 more sources
The circle to lariat ratio of the Ll.LtrB group II intron from Lactococcus lactis is greatly influenced by a variety of biological determinants in vivo. [PDF]
PLoS ONE, 2020 Bacterial group II introns mostly behave as versatile retromobile genetic elements going through distinct cycles of gain and loss. These large RNA molecules are also ribozymes splicing autocatalytically from their interrupted pre-mRNA transcripts by two ...
Caroline Monat, Benoit Cousineau
doaj +2 more sources
Mitogenome of a stink worm (Annelida: Travisiidae) includes degenerate group II intron that is also found in five congeneric species [PDF]
Scientific Reports, 2022 Mitogenomes are useful for inferring phylogenetic relationships between organisms. Although the mitogenomes of Annelida, one of the most morphologically and ecologically diverse metazoan groups have been well sequenced, those of several families remain ...
Genki Kobayashi +2 more
doaj +2 more sources
Group II intron protein localization and insertion sites are affected by polyphosphate. [PDF]
PLoS Biology, 2008 Mobile group II introns consist of a catalytic intron RNA and an intron-encoded protein with reverse transcriptase activity, which act together in a ribonucleoprotein particle to promote DNA integration during intron mobility.
Junhua Zhao +5 more
doaj +7 more sources