Structural basis for the second step of group II intron splicing [PDF]
Nature Communications, 2018 Group II introns are large, self-splicing RNAs that catalyze their own excision from pre-mRNA molecules. Here the authors determine the 3.7 Å crystal structure of the group II intron in the stage immediately before the second step of splicing and present
Russell T. Chan +5 more
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Group II intron inhibits conjugative relaxase expression in bacteria by mRNA targeting [PDF]
eLife, 2018 Group II introns are mobile ribozymes that are rare in bacterial genomes, often cohabiting with various mobile elements, and seldom interrupting housekeeping genes. What accounts for this distribution has not been well understood.
Guosheng Qu +3 more
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Group II intron and repeat-rich red algal mitochondrial genomes demonstrate the dynamic recent history of autocatalytic RNAs [PDF]
BMC Biology, 2022 Background Group II introns are mobile genetic elements that can insert at specific target sequences, however, their origins are often challenging to reconstruct because of rapid sequence decay following invasion and spread into different sites.
Dongseok Kim +5 more
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Group-II intron splicing factors in higher-plants mitochondria [PDF]
Frontiers in Plant Science, 2014 Group-II introns are large catalytic RNAs (ribozymes) which are found in bacteria and organellar genomes of several lower eukaryotes, but are particularly prevalent within the mitochondrial genomes (mtDNA) in plants, where they reside in numerous ...
Gregory G. Brown +2 more
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Mitogenome of a stink worm (Annelida: Travisiidae) includes degenerate group II intron that is also found in five congeneric species [PDF]
Scientific Reports, 2022 Mitogenomes are useful for inferring phylogenetic relationships between organisms. Although the mitogenomes of Annelida, one of the most morphologically and ecologically diverse metazoan groups have been well sequenced, those of several families remain ...
Genki Kobayashi +2 more
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A functional twintron, 'zombie' twintrons and a hypermobile group II intron invading itself in plant mitochondria. [PDF]
Nucleic Acids Res, 2020 The occurrence of group II introns in plant mitochondrial genomes is strikingly different between the six major land plant clades, contrasting their highly conserved counterparts in chloroplast DNA.
Zumkeller S, Gerke P, Knoop V.
europepmc +5 more sources
Visualizing group II intron dynamics between the first and second steps of splicing [PDF]
Nature Communications, 2020 Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition ...
Jacopo Manigrasso +7 more
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Functionality of in vitro reconstituted group II intron RmInt1-derived ribonucleoprotein particles [PDF]
Frontiers in Molecular Biosciences, 2016 The functional unit of mobile group II introns is a ribonucleoprotein particle (RNP) consisting of the intron-encoded protein (IEP) and the excised intron RNA. The IEP has reverse transcriptase activity but also promotes RNA splicing, and the RNA-protein
María Dolores Molina-Sánchez +2 more
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Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. [PDF]
PLoS Genetics, 2015 Mobile bacterial group II introns are evolutionary ancestors of spliceosomal introns and retroelements in eukaryotes. They consist of an autocatalytic intron RNA (a "ribozyme") and an intron-encoded reverse transcriptase, which function together to ...
David M Truong +4 more
doaj +6 more sources
Phylogenetic relationships among group II intron ORFs [PDF]
Nucleic Acids Research, 2001 Group II introns are widely believed to have been ancestors of spliceosomal introns, yet little is known about their own evolutionary history. In order to address the evolution of mobile group II introns, we have compiled 71 open reading frames (ORFs ...
Steven Zimmerly
semanticscholar +5 more sources