Results 121 to 130 of about 775,451 (311)

Characterization of a Group II Intron and its Intron-Encoded Protein

, 2016
Group II introns are a class of mobile genetic elements found in the genomes of bacteria, archaea and in the organellar genomes of some eukaryotes. They are comprised of a large structural RNA with ribozyme activity and a multi-functional intron-encoded protein. The IEP encodes four domains: reverse-transcriptase, maturase, DNA-binding and endonuclease
openaire   +3 more sources

Pathogenicity of Mediator Complex Subunit 27 (MED27) in a Neurodevelopmental Disorder with Cerebellar Atrophy

Advanced Science, EarlyView.
MED27 is one of the 26 subunits in the human Mediator complex (MED). Neurodevelopmental disorder‐causing MED27 genetic variants induce instability of MED, leading to disrupted DNA occupancy, altered chromatin interaction, and subsequent transcriptional dysregulation of critical downstream genes, including master regulatory transcription factors ...
Nuermila Yiliyaer, Xiaocheng Li, Tianyu Guo, Haiying Zhou, Lihai Gong, Luowei Yuan, Yang Fu, Yulong Qiao, Ying Lam Lui, Nuo Chen, Pengfei Lin, Hoi Hung Cheung, Ho Ko, Linyan Meng, Xiao Chen, Yong Lei, Kin Ming Kwan, Huating Wang, Shen Gu   +18 more
wiley   +1 more source

Molecular Chain Elongation Mechanism for n‐Caproate Biosynthesis by Megasphaera Hexanoica

Advanced Science, EarlyView.
The medium‐chain carboxylate‐producing microbe Megasphaera hexanoica is analyzed through genomics, transcriptomics, and metabolic engineering. The n‐caproate biosynthetic pathway is reconstructed in Escherichia coli, highlighting MhTHL as a key β‐ketothiolase. Structural analysis and mutagenesis reveal that MhTHL has a distinct substrate‐binding pocket,
Byoung Seung Jeon, Eun‐Jung Kim, Hogyun Seo, Hyunjin Kim, Seungjin Shin, Caroline Schlaiß, Largus T. Angenent, Kyung‐Jin Kim, Byoung‐In Sang   +8 more
wiley   +1 more source

A Novel Thermostable and Processive Reverse Transcriptase from a Group II Intron of Anoxybacillus flavithermus. [PDF]

Biomolecules, 2023
Oscorbin IP, Filipenko ML.
europepmc   +1 more source

Human Brain Cell‐Type‐Specific Aging Clocks Based on Single‐Nuclei Transcriptomics

Advanced Science, EarlyView.
Muralidharan and colleagues develop cell‐type‐specific transcriptomic aging clocks using single‐nucleus RNA sequencing of human post mortem prefrontal cortex samples. These clocks accurately predict age and identify distinct aging trajectories in specific brain cell types.
Chandramouli Muralidharan, Enikő Zakar‐Polyák, Anita Adami, Anna A. Abbas, Yogita Sharma, Raquel Garza, Jenny G. Johansson, Diahann A. M. Atacho, Éva Renner, Miklós Palkovits, Csaba Kerepesi, Johan Jakobsson, Karolina Pircs   +12 more
wiley   +1 more source

A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron.

Genes & Development, 1997
The Lactococcus lactis group II intron Ll.ltrB is similar to mobile yeast mtDNA group II introns, which encode reverse transcriptase, RNA maturase, and DNA endonuclease activities for site-specific DNA insertion. Here, we show that the Lactococcal intron
M. Matsuura, R. Saldanha, R. Saldanha, Hongwen Ma, Hongwen Ma, H. Wank, H. Wank, Jian Yang, Georg Mohr, Georg Mohr, Stacey Cavanagh, G. Dunny, M. Belfort, A. Lambowitz, A. Lambowitz   +14 more
semanticscholar   +1 more source

Post‐Translational Modifications of TOE3 Regulate Antiviral Defense in Tobacco

Advanced Science, EarlyView.
CK2αL phosphorylates TOE3 and enhances its stability, while FBXL1 promotes the ubiquitination‐degradation of TOE3 via the 26S proteasome. Compared to CK2αL‐phosphorylated TOE3, nonphosphorylated TOE3 shows a much higher affinity for FBXL1 and is more susceptible to ubiquitination‐degradation, thus facilitating viral infection. The regulatory mechanisms
Bolei Jiao, Baijun Wu, Honghui Lin, Dehui Xi   +3 more
wiley   +1 more source

Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.

PLoS ONE, 2013
Some bacterial group II introns are widely used for genetic engineering in bacteria, because they can be reprogrammed to insert into the desired DNA target sites. There is considerable interest in developing this group II intron gene targeting technology
Rafael Nisa-Martínez, Philippe Laporte, José Ignacio Jiménez-Zurdo, Florian Frugier, Martin Crespi, Nicolás Toro   +5 more
doaj   +1 more source

T2T Genomes Unveil Centromere Architecture and Adaptive Divergence in Large Yellow Croaker (Larimichthys crocea)

Advanced Science, EarlyView.
This study presents telomere‐to‐telomere genome assemblies for two populations of Larimichthys crocea. We identified centromere‐specific tandem repeats invaded by LTR/ERV1 retrotransposons, unique 5S rRNA enrichment patterns, and population‐specific structural variants. Comparative genomic analyses further reveal distinct adaptive mechanisms in the MYD
Yu Cui, Yingbo Yuan, Bi Wang, Baolan Wu, Mingyi Cai, Ming Fang, Weiliang Shen, Xiongfei Wu, Fang Han, Zhiyong Wang   +9 more
wiley   +1 more source

Macrophage Zc3h12c Limits Tissue Inflammation and Injury via Alternative Splicing of Pre‐mRNA

Advanced Science, EarlyView.
This work defines the context‐dependent function of Zc3h12c in macrophages using the Tnfrsf11a‐Cre system. It reveals Zc3h12c's dual mechanism—regulating RNA degradation and alternative splicing—with STAT1 as a key target. The findings elucidate a novel post‐transcriptional pathway and identify STAT1 isoforms as potential therapeutic targets for kidney
Chenyu Li, Julian Aurelio Marschner, Yoshihiro Kusunoki, Ningxin Zhang, Xiaoxin Li, Hao Deng, Zhibo Zhao, Kanako Watanabe‐Kusunoki, Zhihui Zhu, Yan Xu, Stefanie Steiger, Maciej Lech, Katalin Susztak, Christian Schulz, Hans‐Joachim Anders   +14 more
wiley   +1 more source