Group II intron-anchored gene deletion in Clostridium. [PDF]
PLoS ONE, 2011 Clostridium plays an important role in commercial and medical use, for which targeted gene deletion is difficult. We proposed an intron-anchored gene deletion approach for Clostridium, which combines the advantage of the group II intron "ClosTron" system
Kaizhi Jia +3 more
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Structural basis for the second step of group II intron splicing [PDF]
Nature Communications, 2018 Group II introns are large, self-splicing RNAs that catalyze their own excision from pre-mRNA molecules. Here the authors determine the 3.7 Å crystal structure of the group II intron in the stage immediately before the second step of splicing and present
Russell T. Chan +5 more
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Engineering Site-Specific Fluorescently-Labeled IEPs to Monitor Group II Intron RNP Assembly [PDF]
Structural DynamicsGroup II introns are self-splicing ribozymes that excise themselves from precursor RNA and mobilize to new DNA loci via retromobility. Central to this mechanism is the intron-encoded protein (IEP), a multidomain maturase and reverse transcriptase that ...
Jasmine A Harper +3 more
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Methylation of rRNA as a host defense against rampant group II intron retrotransposition [PDF]
Mobile DNA, 2021 Background Group II introns are mobile retroelements, capable of invading new sites in DNA. They are self-splicing ribozymes that complex with an intron-encoded protein to form a ribonucleoprotein that targets DNA after splicing.
Justin M. Waldern +8 more
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Group-II intron splicing factors in higher-plants mitochondria [PDF]
Frontiers in Plant Science, 2014 Group-II introns are large catalytic RNAs (ribozymes) which are found in bacteria and organellar genomes of several lower eukaryotes, but are particularly prevalent within the mitochondrial genomes (mtDNA) in plants, where they reside in numerous ...
Gregory G. Brown +2 more
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Group II Introns: Highly Structured yet Dynamic
CHIMIA, 2023 RNA splicing, the removal of introns and ligation of exons, is a crucial process during mRNA maturation. Group II introns are large ribozymes that self-catalyze their splicing, as well as their transposition.
Esra Ahunbay +2 more
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Group II intron inhibits conjugative relaxase expression in bacteria by mRNA targeting [PDF]
eLife, 2018 Group II introns are mobile ribozymes that are rare in bacterial genomes, often cohabiting with various mobile elements, and seldom interrupting housekeeping genes. What accounts for this distribution has not been well understood.
Guosheng Qu +3 more
doaj +2 more sources
Visualizing group II intron dynamics between the first and second steps of splicing [PDF]
Nature Communications, 2020 Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition ...
Jacopo Manigrasso +7 more
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Development of a group II intron‐based genetic manipulation tool for Streptomyces [PDF]
Microbial BiotechnologyThe availability of an alternative and efficient genetic editing technology is critical for fundamental research and strain improvement engineering of Streptomyces species, which are prolific producers of complex secondary metabolites with significant ...
Ziwei Sang +4 more
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Bacterial Group II Intron Genomic Neighborhoods Reflect Survival Strategies: Hiding and Hijacking. [PDF]
Mol Biol Evol, 2020 Waldern J +3 more
europepmc +3 more sources