Group II intron-like reverse transcriptases function in double-strand break repair. [PDF]
Cell, 2022 Bacteria encode free-standing reverse transcriptases (RTs) of unknown function that are closely related to group II intron-encoded RTs. Here, we found that a Pseudomonas aeruginosa group II intron-like RT (G2L4 RT) with YIDD instead of YADD at its active
Park SK +4 more
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Author Correction: Visualizing group II intron dynamics between the first and second steps of splicing. [PDF]
Nat Commun, 2022 Group II introns are ubiquitous self-splicing ribozymes and retrotransposable elements evolutionarily and chemically related to the eukaryotic spliceosome, with potential applications as gene-editing tools.
Manigrasso J +7 more
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Group II intron-based gene targeting reactions in eukaryotes. [PDF]
PLoS ONE, 2008 Mobile group II introns insert site-specifically into DNA target sites by a mechanism termed retrohoming in which the excised intron RNA reverse splices into a DNA strand and is reverse transcribed by the intron-encoded protein.
Marta Mastroianni +7 more
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Crystal structure of a eukaryotic group II intron lariat. [PDF]
Nature, 2014 The formation of branched lariat RNA is an evolutionarily conserved feature of splicing reactions for both group II and spliceosomal introns. The lariat is important for the fidelity of 5′ splice-site selection and consists of a 2′-5′ phosphodiester bond
Robart AR +4 more
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Updated Progress on Group II Intron Splicing Factors in Plant Chloroplasts [PDF]
Current Issues in Molecular Biology, 2022 Group II introns are large catalytic RNAs (ribozymes) in the bacteria and organelle genomes of several lower eukaryotes. Many critical photosynthesis-related genes in the plant chloroplast genome also contain group II introns, and their splicing is ...
Chu Zeng +3 more
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Group II intron as cold sensor for self-preservation and bacterial conjugation. [PDF]
Nucleic Acids Res, 2020 Group II introns are self-splicing ribozymes and mobile genetic elements. Splicing is required for both expression of the interrupted host gene and intron retromobility.
Dong X, Qu G, Piazza CL, Belfort M.
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Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome [PDF]
Frontiers in Molecular Biosciences, 2022 RmInt1 is a group II intron encoding a reverse transcriptase protein (IEP) lacking the C-terminal endonuclease domain. RmInt1 is an efficient mobile retroelement that predominantly reverse splices into the transient single-stranded DNA at the template ...
María Dolores Molina-Sánchez +3 more
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The retrohoming of linear group II intron RNAs in Drosophila melanogaster occurs by both DNA ligase 4-dependent and -independent mechanisms. [PDF]
PLoS Genetics, 2012 Mobile group II introns are bacterial retrotransposons that are thought to have invaded early eukaryotes and evolved into introns and retroelements in higher organisms.
Travis B White, Alan M Lambowitz
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Inverse splicing of a group II intron. [PDF]
Proceedings of the National Academy of Sciences, 1993 I describe the self-splicing of an RNA that consists of exon sequences flanked by group II intron sequences. I find that this RNA undergoes accurate splicing in vitro, yielding an excised exon circle. This splicing reaction involves the joining of the 5' splice site at the end of an exon to the 3' splice site at the beginning of the same exon; thus, I ...
Kevin A. Jarrell
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Exon and protein positioning in a pre-catalytic group II intron RNP primed for splicing. [PDF]
Nucleic Acids Res, 2020 Group II introns are the putative progenitors of nuclear spliceosomal introns and use the same two-step splicing pathway. In the cell, the intron RNA forms a ribonucleoprotein (RNP) complex with the intron-encoded protein (IEP), which is essential for ...
Liu N +7 more
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