Crystal structure of a eukaryotic group II intron lariat. [PDF]
Nature, 2014 The formation of branched lariat RNA is an evolutionarily conserved feature of splicing reactions for both group II and spliceosomal introns. The lariat is important for the fidelity of 5' splice-site selection and consists of a 2'-5' phosphodiester bond between a bulged adenosine and the 5' end of the intron.
Robart AR +4 more
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Updated Progress on Group II Intron Splicing Factors in Plant Chloroplasts [PDF]
Current Issues in Molecular Biology, 2022 Group II introns are large catalytic RNAs (ribozymes) in the bacteria and organelle genomes of several lower eukaryotes. Many critical photosynthesis-related genes in the plant chloroplast genome also contain group II introns, and their splicing is ...
Chu Zeng +3 more
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Group II intron as cold sensor for self-preservation and bacterial conjugation. [PDF]
Nucleic Acids Res, 2020 Group II introns are self-splicing ribozymes and mobile genetic elements. Splicing is required for both expression of the interrupted host gene and intron retromobility.
Dong X, Qu G, Piazza CL, Belfort M.
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Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome [PDF]
Frontiers in Molecular Biosciences, 2022 RmInt1 is a group II intron encoding a reverse transcriptase protein (IEP) lacking the C-terminal endonuclease domain. RmInt1 is an efficient mobile retroelement that predominantly reverse splices into the transient single-stranded DNA at the template ...
María Dolores Molina-Sánchez +3 more
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Exon and protein positioning in a pre-catalytic group II intron RNP primed for splicing. [PDF]
Nucleic Acids Res, 2020 Group II introns are the putative progenitors of nuclear spliceosomal introns and use the same two-step splicing pathway. In the cell, the intron RNA forms a ribonucleoprotein (RNP) complex with the intron-encoded protein (IEP), which is essential for ...
Liu N +7 more
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Inverse splicing of a group II intron. [PDF]
Proceedings of the National Academy of Sciences, 1993 I describe the self-splicing of an RNA that consists of exon sequences flanked by group II intron sequences. I find that this RNA undergoes accurate splicing in vitro, yielding an excised exon circle. This splicing reaction involves the joining of the 5' splice site at the end of an exon to the 3' splice site at the beginning of the same exon; thus, I ...
Kevin A. Jarrell
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A functional twintron, 'zombie' twintrons and a hypermobile group II intron invading itself in plant mitochondria. [PDF]
Nucleic Acids Res, 2020 The occurrence of group II introns in plant mitochondrial genomes is strikingly different between the six major land plant clades, contrasting their highly conserved counterparts in chloroplast DNA.
Zumkeller S, Gerke P, Knoop V.
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The circle to lariat ratio of the Ll.LtrB group II intron from Lactococcus lactis is greatly influenced by a variety of biological determinants in vivo. [PDF]
PLoS ONE, 2020 Bacterial group II introns mostly behave as versatile retromobile genetic elements going through distinct cycles of gain and loss. These large RNA molecules are also ribozymes splicing autocatalytically from their interrupted pre-mRNA transcripts by two ...
Caroline Monat, Benoit Cousineau
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Bacterial Group II Intron Genomic Neighborhoods Reflect Survival Strategies: Hiding and Hijacking. [PDF]
Mol Biol Evol, 2020 Group II (gII) introns are mobile retroelements that can spread to new DNA sites through retrotransposition, which can be influenced by a variety of host factors.
Waldern J +3 more
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Group II Intron RNPs and Reverse Transcriptases: From Retroelements to Research Tools. [PDF]
Cold Spring Harb Perspect Biol, 2019 SUMMARYGroup II introns, self-splicing retrotransposons, serve as both targets of investigation into their structure, splicing, and retromobility and a source of tools for genome editing and RNA analysis.
Belfort M, Lambowitz AM.
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