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Excision of Group II Introns as Circles [PDF]
Molecular Cell, 2001 Group II introns are usually removed from precursor RNAs as lariats comprised of a circular component and a short 3' tail. We find that group II introns can also be excised as complete circles. Circle formation requires release of the 3' exon of a splicing substrate, apparently by a trans splicing mechanism.
Murray, Heather L. +6 more
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The trouble with (group II) introns [PDF]
Proceedings of the National Academy of Sciences, 2014 Qu et al. provide new grist for the introns origin mill (1), adding one more chapter to what has become a very long narrative. Back in the day when spliceosomal introns in the protein-coding genes of eukaryotes were still very new to science (1977–1978), some of us argued that—in an evolutionary sense—they were actually very old (2, 3).
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Exon-intron structure and sequence variation of the calreticulin gene among Rhipicephalus sanguineus group ticks [PDF]
, 2016 Background: Calreticulin proteins (CRTs) are important components of tick saliva, which is involved in the blood meal success, pathogen transmission and host allergic responses.
Dantas-Torres, Filipe +6 more
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Single-molecule studies of group II intron ribozymes [PDF]
Proceedings of the National Academy of Sciences, 2008 Group II intron ribozymes fold into their native structure by a unique stepwise process that involves an initial slow compaction followed by fast formation of the native state in a Mg 2+ -dependent manner. Single-molecule fluorescence reveals three distinct on-pathway conformations in dynamic equilibrium connected ...
Steiner, M +3 more
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The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]
, 1992 The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert +3 more
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Circularization pathway of a bacterial group II intron. [PDF]
Nucleic Acids Res, 2016 Group II introns are large RNA enzymes that can excise as lariats, circles or in a linear form through branching, circularization or hydrolysis, respectively. Branching is by far the main and most studied splicing pathway while circularization was mostly overlooked. We previously showed that removal of the branch point A residue from Ll.LtrB, the group
Monat C, Cousineau B.
europepmc +4 more sources
Group II intron splicing factors in plant mitochondria [PDF]
Frontiers in Plant Science, 2014 Group II introns are large catalytic RNAs (ribozymes) which are found in bacteria and organellar genomes of several lower eukaryotes, but are particularly prevalent within the mitochondrial genomes (mtDNA) in plants, where they reside in numerous critical genes. Their excision is therefore essential for mitochondria biogenesis and respiratory functions,
Brown, Gregory G. +2 more
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Protein-Facilitated Folding of Group II Intron Ribozymes [PDF]
Journal of Molecular Biology, 2010 Multiple studies hypothesize that DEAD-box proteins facilitate folding of the ai5gamma group II intron. However, these conclusions are generally inferred from splicing kinetics, and not from direct monitoring of DEAD-box protein-facilitated folding of the intron. Using native gel electrophoresis and dimethyl sulfate structural probing, we monitored Mss-
Olga, Fedorova +2 more
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RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts. [PDF]
, 2011 Over 50% of genes in Plasmodium falciparum, the deadliest human malaria parasite, contain predicted introns, yet experimental characterization of splicing in this organism remains incomplete.
DeRisi, Joseph L +2 more
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Cryptic MHC Polymorphism Revealed but Not Explained by Selection on the Class IIB Peptide-Binding Region [PDF]
, 2012 The immune genes of the major histocompatibility complex (MHC) are characterized by extraordinarily high levels of nucleotide and haplotype diversity. This variation is maintained by pathogen-mediated balancing selection that is operating on the peptide ...
Barcaccia +32 more
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