Results 41 to 50 of about 364,363 (199)

Circularization pathway of a bacterial group II intron [PDF]

Nucleic Acids Research, 2015
Group II introns are large RNA enzymes that can excise as lariats, circles or in a linear form through branching, circularization or hydrolysis, respectively. Branching is by far the main and most studied splicing pathway while circularization was mostly overlooked. We previously showed that removal of the branch point A residue from Ll.LtrB, the group
Benoit Cousineau, Caroline Monat
openaire   +3 more sources

Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease. [PDF]

PLoS ONE, 2016
In Chaetomium thermophilum (DSM 1495) within the mitochondrial DNA (mtDNA) small ribosomal subunit (rns) gene a group IIA1 intron interrupts an open reading frame (ORF) encoded within a group I intron (mS1247).
Tuhin Kumar Guha, Georg Hausner
doaj   +1 more source

ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2

Frontiers in Plant Science, 2022
Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown ...
Kaijian Fan, Qinghui Fu, Qianhan Wei, Sinian Jia, Anqi Zhao, Tengteng Wang, Jie Cao, Yan Liu, Zhenjing Ren, Yunjun Liu   +9 more
doaj   +1 more source

Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming. [PDF]

PLoS Genetics, 2013
Mobile group II introns retrohome by an RNP-based mechanism in which the intron RNA reverse splices into a DNA site and is reverse transcribed by the associated intron-encoded protein.
Jun Yao, David M Truong, Alan M Lambowitz   +2 more
doaj   +1 more source

The group II intron ribonucleoprotein precursor is a large, loosely packed structure [PDF]

, 2011
Group II self-splicing introns are phylogenetically diverse retroelements that are widely held to be the ancestors of spliceosomal introns and retrotransposons that insert into DNA.
Azubel, Belfort, Blocker, Boehringer, Cavalier-Smith, Cech, Chalamcharla, Chanfreau, Costa, Cousineau, Dai, Dai, de Ruyter, Eickbush, Frank, Grassucci, Gregory D. Van Duyne, Halvorsen, Hayes, Hetzer, Joachim Frank, Kar, Kushol Gupta, Lambowitz, Lydia M. Contreras-Martin, Marco, Marlene Belfort, Matsuura, Matsuura, Mills, Mills, Penczek, Pyle, Pyle, Radermacher, Robert A. Grassucci, Saldanha, Schuck, Shaikh, Shaikh, Sharp, Shukla, Siatecka, Slagter-Jäger, Sperling, Staley, Stoffler, Stoffler, Tanvir R. Shaikh, Tao Huang, Toor, Voss, Wank   +52 more
core   +2 more sources

Mechanisms used for genomic proliferation by thermophilic group II introns. [PDF]

PLoS Biology, 2010
Mobile group II introns, which are found in bacterial and organellar genomes, are site-specific retroelements hypothesized to be evolutionary ancestors of spliceosomal introns and retrotransposons in higher organisms.
Georg Mohr, Eman Ghanem, Alan M Lambowitz   +2 more
doaj   +1 more source

Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome

Frontiers in Molecular Biosciences, 2022
RmInt1 is a group II intron encoding a reverse transcriptase protein (IEP) lacking the C-terminal endonuclease domain. RmInt1 is an efficient mobile retroelement that predominantly reverse splices into the transient single-stranded DNA at the template ...
María Dolores Molina-Sánchez, Fernando Manuel García-Rodríguez, Eduardo Andrés-León, Nicolás Toro   +3 more
doaj   +1 more source

Group II intron mobility occurs by target DNA-primed reverse transcription [PDF]

, 1995
Mobile group II introns encode reverse transcriptases and insert site specifically into intronless alleles (homing). Here, in vitro experiments show that homing of the yeast mtDNA group II intron a12 occurs by reverse transcription at a double-strand ...
Guo, Huatao, Lambowltz, Alan M., Perlman, Philip S., Zimmerly, Steven   +3 more
core   +1 more source

Bacterial group II introns: not just splicing [PDF]

FEMS Microbiology Reviews, 2007
Group II introns are both catalytic RNAs (ribozymes) and mobile retroelements that were discovered almost 14 years ago. It has been suggested that eukaryotic mRNA introns might have originated from the group II introns present in the alphaproteobacterial progenitor of the mitochondria.
José I. Jiménez-Zurdo, Fernando M. García-Rodríguez, Nicolás Toro   +2 more
openaire   +3 more sources

Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen. [PDF]

PLoS ONE, 2012
BACKGROUND: Group II introns are RNA enzymes that splice themselves from pre-mRNA transcripts. Most bacterial group II introns harbour an open reading frame (ORF), coding for a protein with reverse transcriptase, maturase and occasionally DNA binding and
Christine Ritlop, Caroline Monat, Benoit Cousineau   +2 more
doaj   +1 more source