Nearest neighbour spacing distribution of basis in some intron-less and intron-containing DNA sequences [PDF]
We show that the nearest neighbour distribution of distances between basis pairs of some intron-less and intron-containing coding regions are the same when a procedure, called {\em unfolding}, is applied. Such a procedure consists in separating the secular variations from the oscillatory terms.
arxiv +1 more source
tRNA-isoleucine-tryptophan Composite Gene [PDF]
Transfer-RNA genes in archaea often have introns intervening between exon sequences. The structural motif at the boundary between exon and intron is the bulge-helix-bulge. Computational investigations of these boundary structures in H. marismortui lead us to propose that tRNA-isoleucine and tRNA-tryptophan genes are co-located.
arxiv +1 more source
Single-molecule studies of group II intron ribozymes [PDF]
Group II intron ribozymes fold into their native structure by a unique stepwise process that involves an initial slow compaction followed by fast formation of the native state in a Mg 2+ -dependent manner. Single-molecule fluorescence reveals three distinct on-pathway conformations in dynamic equilibrium connected ...
Steiner, M+3 more
openaire +3 more sources
Mitogenomes are useful for inferring phylogenetic relationships between organisms. Although the mitogenomes of Annelida, one of the most morphologically and ecologically diverse metazoan groups have been well sequenced, those of several families remain ...
Genki Kobayashi+2 more
doaj +1 more source
Stability domains of actin genes and genomic evolution [PDF]
In eukaryotic genes the protein coding sequence is split into several fragments, the exons, separated by non-coding DNA stretches, the introns. Prokaryotes do not have introns in their genome. We report the calculations of stability domains of actin genes for various organisms in the animal, plant and fungi kingdoms.
arxiv +1 more source
Nuclear expression of a group II intron is consistent with spliceosomal intron ancestry [PDF]
Group II introns are self-splicing RNAs found in eubacteria, archaea, and eukaryotic organelles. They are mechanistically similar to the metazoan nuclear spliceosomal introns; therefore, group II introns have been invoked as the progenitors of the eukaryotic pre-mRNA introns.
Venkata R. Chalamcharla+2 more
openaire +2 more sources
Group II intron in Bacillus cereus has an unusual 3′ extension and splices 56 nucleotides downstream of the predicted site [PDF]
All group II introns known to date fold into six functional domains. However, we recently identified an intron in Bacillus cereus ATCC 10987, B.c.I4, that splices 56 nt downstream of the expected 3′ splice site in vivo (Tourasse et al. 2005, J. Bacteriol.
Anne-Brit Kolstø+36 more
core +2 more sources
A group III intron is formed from domains of two individual group II introns. [PDF]
A 1352-nucleotide intron within the Euglena gracilis chloroplast ycf8 gene has been characterized as a complex twintron with overlapping internal introns and alternative splicing pathways. Partially spliced pre-mRNAs were characterized by a combination of cDNA cloning and sequencing, Northern hybridization, and S1 nuclease protection analyses.
Richard B. Hallick, Ling Hong
openaire +2 more sources
The association of group IIB intron with integrons in hypersaline environments
Background Group II introns are mobile genetic elements used as efficient gene targeting tools. They function as both ribozymes and retroelements. Group IIC introns are the only class reported so far to be associated with integrons.
Sarah Sonbol, Rania Siam
doaj +1 more source
Mechanism of DNA target site recognition by group II introns TeI3c and GsI-IIC and splicing activity of GsI-IIC reverse transcriptase [PDF]
Mobile group II introns are self-catalytic ribozymes found in bacteria and eukaryotic organelles. They can mobilize within the genomes by retrohoming, which involves RNA-catalyzed splicing followed by the excised intron reverse splicing into a target ...
Kang, Sean Yoon-Seo
core +1 more source