The trouble with (group II) introns [PDF]
Proceedings of the National Academy of Sciences, 2014 Qu et al. provide new grist for the introns origin mill (1), adding one more chapter to what has become a very long narrative. Back in the day when spliceosomal introns in the protein-coding genes of eukaryotes were still very new to science (1977–1978), some of us argued that—in an evolutionary sense—they were actually very old (2, 3).
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Single-molecule studies of group II intron ribozymes [PDF]
Proceedings of the National Academy of Sciences, 2008 Group II intron ribozymes fold into their native structure by a unique stepwise process that involves an initial slow compaction followed by fast formation of the native state in a Mg 2+ -dependent manner. Single-molecule fluorescence reveals three distinct on-pathway conformations in dynamic equilibrium connected ...
Steiner, M +3 more
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Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming. [PDF]
PLoS Genetics, 2013 Mobile group II introns retrohome by an RNP-based mechanism in which the intron RNA reverse splices into a DNA site and is reverse transcribed by the associated intron-encoded protein.
Jun Yao +2 more
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Mechanisms used for genomic proliferation by thermophilic group II introns. [PDF]
PLoS Biology, 2010 Mobile group II introns, which are found in bacterial and organellar genomes, are site-specific retroelements hypothesized to be evolutionary ancestors of spliceosomal introns and retrotransposons in higher organisms.
Georg Mohr +2 more
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Circularization pathway of a bacterial group II intron. [PDF]
Nucleic Acids Res, 2016 Group II introns are large RNA enzymes that can excise as lariats, circles or in a linear form through branching, circularization or hydrolysis, respectively. Branching is by far the main and most studied splicing pathway while circularization was mostly overlooked. We previously showed that removal of the branch point A residue from Ll.LtrB, the group
Monat C, Cousineau B.
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Multiplex ligation-dependent probe amplification (MLPA) analysis is an effective tool for the detection of novel intragenic PLA2G6 mutations: Implications for molecular diagnosis [PDF]
, 2010 Phospholipase associated neurodegeneration (PLAN) comprises a heterogeneous group of autosomal recessive neurological disorders caused by mutations in the PLA2G6 gene. Direct gene sequencing detects 85% mutations in infantile neuroaxonal dystrophy.
Brady, Angela F +14 more
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Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen. [PDF]
PLoS ONE, 2012 BACKGROUND: Group II introns are RNA enzymes that splice themselves from pre-mRNA transcripts. Most bacterial group II introns harbour an open reading frame (ORF), coding for a protein with reverse transcriptase, maturase and occasionally DNA binding and
Christine Ritlop +2 more
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Intracellular interferons in fish : a unique means to combat viral infection [PDF]
, 2013 Peer reviewedPublisher ...
A Lopez-Munoz +45 more
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Protein-Facilitated Folding of Group II Intron Ribozymes [PDF]
Journal of Molecular Biology, 2010 Multiple studies hypothesize that DEAD-box proteins facilitate folding of the ai5gamma group II intron. However, these conclusions are generally inferred from splicing kinetics, and not from direct monitoring of DEAD-box protein-facilitated folding of the intron. Using native gel electrophoresis and dimethyl sulfate structural probing, we monitored Mss-
Olga, Fedorova +2 more
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The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]
, 1992 The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert +3 more
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