Results 61 to 70 of about 838,914 (323)

Bacterial group II introns generate genetic diversity by circularization and trans-splicing from a population of intron-invaded mRNAs. [PDF]

PLoS Genetics, 2018
Group II introns are ancient retroelements that significantly shaped the origin and evolution of contemporary eukaryotic genomes. These self-splicing ribozymes share a common ancestor with the telomerase enzyme, the spliceosome machinery as well as the ...
Félix LaRoche-Johnston, Caroline Monat, Samy Coulombe, Benoit Cousineau   +3 more
doaj   +1 more source

Rapid Targeted Gene Disruption in Bacillus Anthracis [PDF]

, 2013
Anthrax is a zoonotic disease recognized to affect herbivores since Biblical times and has the widest range of susceptible host species of any known pathogen.
Ellington, Andrew, Kramer, Ryan, Lambowitz, Alan M., Lamkin, Thomas J., Pemberton, Adin, Perutka, Jiri, Saldanha, Roland J., Shiflett, Patrick, Taylor, Deborah, Whitt, Jacob T.   +9 more
core   +3 more sources

The trouble with (group II) introns [PDF]

Proceedings of the National Academy of Sciences, 2014
Qu et al. provide new grist for the introns origin mill (1), adding one more chapter to what has become a very long narrative. Back in the day when spliceosomal introns in the protein-coding genes of eukaryotes were still very new to science (1977–1978), some of us argued that—in an evolutionary sense—they were actually very old (2, 3).
openaire   +3 more sources

Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease. [PDF]

PLoS ONE, 2016
In Chaetomium thermophilum (DSM 1495) within the mitochondrial DNA (mtDNA) small ribosomal subunit (rns) gene a group IIA1 intron interrupts an open reading frame (ORF) encoded within a group I intron (mS1247).
Tuhin Kumar Guha, Georg Hausner
doaj   +1 more source

ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2

Frontiers in Plant Science, 2022
Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown ...
Kaijian Fan, Qinghui Fu, Qianhan Wei, Sinian Jia, Anqi Zhao, Tengteng Wang, Jie Cao, Yan Liu, Zhenjing Ren, Yunjun Liu   +9 more
doaj   +1 more source

Mitochondrial and chloroplast DNA-based phylogeny of Pelargonium (Geraniaceae) [PDF]

, 2000
Overall phylogenetic relationships within the genus Pelargonium (Geraniaceae) were inferred based on DNA sequences from mitochondrial(mt)-encoded nad1 b/c exons and from chloroplast(cp)-encoded trnL (UAA) 5' exon-trnF (GAA) exon regions using two species
Bakker, Freek T., Culham, Alastair, Gibby, Mary, Pankhurst, Clive E.   +3 more
core   +2 more sources

Elimination of a group II intron from a plastid gene causes a mutant phenotype [PDF]

, 2011
Group II introns are found in bacteria and cell organelles (plastids, mitochondria) and are thought to represent the evolutionary ancestors of spliceosomal introns.
Belfort, Bock, Bonen, Christina Thomson, Copertino, Costa, D. Karcher, Dai, de Longevialle, Durrenberger, Eberhard, Hess, Hetzer, Hirose, Jenkins, K. Petersen, Karcher, Kramer, Kuhsel, M. A. Schottler, Michel, Mohr, Ostheimer, Padgett, Perlman, Pyle, R. Bock, Schottler, Scott, Svab, Vaughn, W. Thiele, Winkler, Zhang   +33 more
core   +2 more sources

Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming. [PDF]

PLoS Genetics, 2013
Mobile group II introns retrohome by an RNP-based mechanism in which the intron RNA reverse splices into a DNA site and is reverse transcribed by the associated intron-encoded protein.
Jun Yao, David M Truong, Alan M Lambowitz   +2 more
doaj   +1 more source

High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases

RNA: A publication of the RNA Society, 2016
Next-generation RNA-sequencing (RNA-seq) has revolutionized transcriptome profiling, gene expression analysis, and RNA-based diagnostics. Here, we developed a new RNA-seq method that exploits thermostable group II intron reverse transcriptases (TGIRTs ...
Yidan Qin, Jun Yao, Douglas C. Wu, Ryan M. Nottingham, S. Mohr, S. Hunicke-Smith, A. Lambowitz   +6 more
semanticscholar   +1 more source

Mechanisms used for genomic proliferation by thermophilic group II introns. [PDF]

PLoS Biology, 2010
Mobile group II introns, which are found in bacterial and organellar genomes, are site-specific retroelements hypothesized to be evolutionary ancestors of spliceosomal introns and retrotransposons in higher organisms.
Georg Mohr, Eman Ghanem, Alan M Lambowitz   +2 more
doaj   +1 more source