Results 71 to 80 of about 775,451 (311)
Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen. [PDF]
BACKGROUND: Group II introns are RNA enzymes that splice themselves from pre-mRNA transcripts. Most bacterial group II introns harbour an open reading frame (ORF), coding for a protein with reverse transcriptase, maturase and occasionally DNA binding and
Christine Ritlop +2 more
doaj +1 more source
Group II intron protein localization and insertion sites are affected by polyphosphate. [PDF]
Mobile group II introns consist of a catalytic intron RNA and an intron-encoded protein with reverse transcriptase activity, which act together in a ribonucleoprotein particle to promote DNA integration during intron mobility.
Junhua Zhao +5 more
doaj +1 more source
Single-molecule studies of group II intron ribozymes [PDF]
Group II intron ribozymes fold into their native structure by a unique stepwise process that involves an initial slow compaction followed by fast formation of the native state in a Mg 2+ -dependent manner. Single-molecule fluorescence reveals three distinct on-pathway conformations in dynamic equilibrium connected ...
Steiner, M +3 more
openaire +3 more sources
Nuclear expression of a group II intron is consistent with spliceosomal intron ancestry [PDF]
Group II introns are self-splicing RNAs found in eubacteria, archaea, and eukaryotic organelles. They are mechanistically similar to the metazoan nuclear spliceosomal introns; therefore, group II introns have been invoked as the progenitors of the eukaryotic pre-mRNA introns.
Venkata R. Chalamcharla +2 more
openaire +2 more sources
The association of group IIB intron with integrons in hypersaline environments
Background Group II introns are mobile genetic elements used as efficient gene targeting tools. They function as both ribozymes and retroelements. Group IIC introns are the only class reported so far to be associated with integrons.
Sarah Sonbol, Rania Siam
doaj +1 more source
A group III intron is formed from domains of two individual group II introns. [PDF]
A 1352-nucleotide intron within the Euglena gracilis chloroplast ycf8 gene has been characterized as a complex twintron with overlapping internal introns and alternative splicing pathways. Partially spliced pre-mRNAs were characterized by a combination of cDNA cloning and sequencing, Northern hybridization, and S1 nuclease protection analyses.
Richard B. Hallick, Ling Hong
openaire +2 more sources
Polymorphism of the rps16 gene intron in Malus Mill. and related Rosaceae Juss. species
The intron of the chloroplast rps16 gene encoding ribosomal protein 16S belongs to group II introns, which have the capacity to self-splice. The sequence of this intron may be quite polymorphic and is widely used for molecular-genetic studies of ...
M. A. Filyushin, K. V. Boris
doaj +1 more source
Group II introns break new boundaries: presence in a bilaterian's genome. [PDF]
Group II introns are ribozymes, removing themselves from their primary transcripts, as well as mobile genetic elements, transposing via an RNA intermediate, and are thought to be the ancestors of spliceosomal introns. Although common in bacteria and most
Yvonne Vallès +2 more
doaj +1 more source
To understand the evolution of Ulva chloroplast genomes at intraspecific and interspecific levels, in this study, three complete chloroplast genomes of Ulva compressa Linnaeus were sequenced and compared with the available Ulva cpDNA data.
Feng Liu +3 more
doaj +1 more source
Pseudo-nitzschia is an important genus of diatoms with many species capable of inducing harmful algae blooms (HABs) in coastal and oceanic waters, some of which produce the toxin domoic acid (DA), a neurotoxin that causes amnesic shellfish poisoning (ASP)
Ziyan He +6 more
doaj +1 more source

