Results 71 to 80 of about 838,914 (323)

RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase

RNA: A publication of the RNA Society, 2016
Next-generation RNA sequencing (RNA-seq) has revolutionized our ability to analyze transcriptomes. Current RNA-seq methods are highly reproducible, but each has biases resulting from different modes of RNA sample preparation, reverse transcription, and ...
Ryan M. Nottingham, Douglas C. Wu, Yidan Qin, Jun Yao, S. Hunicke-Smith, A. Lambowitz   +5 more
semanticscholar   +1 more source

Bacterial group II introns: not just splicing [PDF]

FEMS Microbiology Reviews, 2007
Group II introns are both catalytic RNAs (ribozymes) and mobile retroelements that were discovered almost 14 years ago. It has been suggested that eukaryotic mRNA introns might have originated from the group II introns present in the alphaproteobacterial progenitor of the mitochondria.
José I. Jiménez-Zurdo, Fernando M. García-Rodríguez, Nicolás Toro   +2 more
openaire   +3 more sources

The group II intron ribonucleoprotein precursor is a large, loosely packed structure [PDF]

, 2011
Group II self-splicing introns are phylogenetically diverse retroelements that are widely held to be the ancestors of spliceosomal introns and retrotransposons that insert into DNA.
Azubel, Belfort, Blocker, Boehringer, Cavalier-Smith, Cech, Chalamcharla, Chanfreau, Costa, Cousineau, Dai, Dai, de Ruyter, Eickbush, Frank, Grassucci, Gregory D. Van Duyne, Halvorsen, Hayes, Hetzer, Joachim Frank, Kar, Kushol Gupta, Lambowitz, Lydia M. Contreras-Martin, Marco, Marlene Belfort, Matsuura, Matsuura, Mills, Mills, Penczek, Pyle, Pyle, Radermacher, Robert A. Grassucci, Saldanha, Schuck, Shaikh, Shaikh, Sharp, Shukla, Siatecka, Slagter-Jäger, Sperling, Staley, Stoffler, Stoffler, Tanvir R. Shaikh, Tao Huang, Toor, Voss, Wank   +52 more
core   +2 more sources

Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen. [PDF]

PLoS ONE, 2012
BACKGROUND: Group II introns are RNA enzymes that splice themselves from pre-mRNA transcripts. Most bacterial group II introns harbour an open reading frame (ORF), coding for a protein with reverse transcriptase, maturase and occasionally DNA binding and
Christine Ritlop, Caroline Monat, Benoit Cousineau   +2 more
doaj   +1 more source

The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]

, 1992
The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert, Goris, Anne, Neefs, Jean-Marc, Van de Peer, Yves   +3 more
core   +1 more source

DNA-based S-genotyping of Japanese plum and pluot cultivars to clarify incompatibility relationships [PDF]

, 2007
Diploid japanese plum (Prunus salicina Lindl.) cultivars are commonly self-incompatible. To date, 14 incompatibility alleles (S-alleles) have been identified and labeled with alphabetical (S-a-S-n) and 5 with numeric codes (S-1, S-3-S-6).
Halász, Júlia, Hegedűs, Attila, Pedryc, Andrzej, Szabó, Zoltán   +3 more
core   +1 more source

Single-molecule studies of group II intron ribozymes [PDF]

Proceedings of the National Academy of Sciences, 2008
Group II intron ribozymes fold into their native structure by a unique stepwise process that involves an initial slow compaction followed by fast formation of the native state in a Mg 2+ -dependent manner. Single-molecule fluorescence reveals three distinct on-pathway conformations in dynamic equilibrium connected ...
Steiner, M, Karunatilaka, K S, Sigel, Roland K O, Rueda, D   +3 more
openaire   +3 more sources

Nuclear expression of a group II intron is consistent with spliceosomal intron ancestry [PDF]

Genes & Development, 2010
Group II introns are self-splicing RNAs found in eubacteria, archaea, and eukaryotic organelles. They are mechanistically similar to the metazoan nuclear spliceosomal introns; therefore, group II introns have been invoked as the progenitors of the eukaryotic pre-mRNA introns.
Venkata R. Chalamcharla, Marlene Belfort, M. Joan Curcio   +2 more
openaire   +2 more sources

The association of group IIB intron with integrons in hypersaline environments

Mobile DNA, 2021
Background Group II introns are mobile genetic elements used as efficient gene targeting tools. They function as both ribozymes and retroelements. Group IIC introns are the only class reported so far to be associated with integrons.
Sarah Sonbol, Rania Siam
doaj   +1 more source

A group III intron is formed from domains of two individual group II introns. [PDF]

Genes & Development, 1994
A 1352-nucleotide intron within the Euglena gracilis chloroplast ycf8 gene has been characterized as a complex twintron with overlapping internal introns and alternative splicing pathways. Partially spliced pre-mRNAs were characterized by a combination of cDNA cloning and sequencing, Northern hybridization, and S1 nuclease protection analyses.
Richard B. Hallick, Ling Hong
openaire   +2 more sources