Results 51 to 60 of about 35,755 (265)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Intermodal group velocity dispersion of few-mode fiber

IEICE Electronics Express, 2010
The intermodal dispersion of few-mode fiber limits the propagation distance of mode division multiplexing (MDM). We numerically investigate the propagation constant and group velocity of few-mode fibers. The refractive index profile of few-mode fibers should be designed to achieve long and dense MDM transmissions with digital coherent receivers.
Hirokazu Kubota, Hidehito Takara, Tadao Nakagawa, Munehiro Matsui, Toshio Morioka   +4 more
openaire   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Group index and group velocity dispersion in silicon-on-insulator photonic wires

Optics Express, 2006
We determine group index and group velocity dispersion (GVD) of SOI single-mode strip waveguides (photonic wires) with 525×226nm cross-section over the entire telecommunication bandwidth by employing an integrated Mach-Zehnder interferometer. The measured GVD yields 4400 ps/(nm∙km) at 1550 nm and exceeds that of standard single-mode fibers by almost ...
Eric, Dulkeith, Fengnian, Xia, Laurent, Schares, William M J, Green, Yurii A, Vlasov   +4 more
openaire   +2 more sources

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Femtosecond filamentation of optical vortex in a medium with anomalous group velocity dispersion

EPJ Web of Conferences, 2017
Within the framework of the slowly varying wave approximation method, the pictures of spatio-temporal dynamics of femtosecond pulse in a ring beam with phase singularity were obtained during propagation in CaF2 crystal with anomalous group velocity ...
Vasilyev Evgeny, Shlenov Svyatoslav, Kandidov Valery   +2 more
doaj   +1 more source

A Feasibility Study on Monitoring Crustal Structure Variations by Direct Comparison of Surface Wave Dispersion Curves from Ambient Seismic Noise

International Journal of Geophysics, 2020
This work assesses the feasibility of the direct use of surface-wave dispersion curves from seismic ambient noise to gain insight into the crustal structure of Bransfield Strait and detect seasonal seismic velocity changes. We cross-correlated four years
K. Muhumuza
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source