Results 51 to 60 of about 35,426 (265)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
International Journal of Geophysics, 2020 This work assesses the feasibility of the direct use of surface-wave dispersion curves from seismic ambient noise to gain insight into the crustal structure of Bransfield Strait and detect seasonal seismic velocity changes. We cross-correlated four years
K. Muhumuza
doaj +1 more source
Group index and group velocity dispersion in silicon-on-insulator photonic wires
Optics Express, 2006 We determine group index and group velocity dispersion (GVD) of SOI single-mode strip waveguides (photonic wires) with 525x226nm cross-section over the entire telecommunication bandwidth by employing an integrated Mach-Zehnder interferometer. The measured GVD yields 4400 ps/(nm*km) at 1550 nm and exceeds that of standard single-mode fibers by almost ...
Eric, Dulkeith +4 more
openaire +2 more sources
The concentration-velocity dispersion relation in galaxy groups [PDF]
Monthly Notices of the Royal Astronomical Society, 2007 12 pages, 11 figures, published in ...
Faltenbacher, Andreas +1 more
openaire +2 more sources
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Femtosecond filamentation of optical vortex in a medium with anomalous group velocity dispersion
EPJ Web of Conferences, 2017 Within the framework of the slowly varying wave approximation method, the pictures of spatio-temporal dynamics of femtosecond pulse in a ring beam with phase singularity were obtained during propagation in CaF2 crystal with anomalous group velocity ...
Vasilyev Evgeny +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Zero dispersion Kerr solitons in optical microresonators
Nature Communications, 2022 Here, the authors find the missing link for soliton microcombs that exist at the boundary where the group velocity dispersion of light changes sign: zero-dispersion solitons.
Miles H. Anderson +5 more
doaj +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source