Histochemistry of guanylate cyclase activity. [PDF]
Journal of Histochemistry & Cytochemistry, 1995 Guanylate cyclase liberates pyrophosphate from guanosine triphosphate (GTP). In studies published previously, this phosphate is trapped by lead ions even though it is known that free lead ions inactivate a considerable proportion of this enzymatic activity.
Lucille Bitensky +5 more
openaire +3 more sources
cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+ [PDF]
, 1985 cAMP induces a transient increase of cAMP and cGMP levels in Dictyostelium discoideum cells. Fast binding experiments reveal three types of cAMP-binding site (S, H and L), which have different off-rates (t0.5, 0.7-15 s) and different affinities (Kd, 15 ...
Brachet +25 more
core +3 more sources
Dysfunction of outer segment guanylate cyclase caused by retinal disease related mutations
Frontiers in Molecular Neuroscience, 2014 Membrane bound guanylate cyclases are expressed in rod and cone cells of the vertebrate retina and mutations in several domains of rod outer segment guanylate cyclase 1 (ROS-GC1 encoded by the gene GUCY2D) correlate with different forms of retinal ...
Patrick eZägel, Karl-Wilhelm eKoch
doaj +1 more source
Integrative dynamic structural biology unveils conformers essential for the oligomerization of a large GTPase [PDF]
, 2020 Guanylate binding proteins (GBPs) are soluble dynamin-like proteins with structured domains that undergo a conformational transition for GTP-controlled oligomerization to exert their function as part of the innate immune system of mammalian cells - attacking intra-cellular parasites by disrupting their membranes.
arxiv +1 more source
Regulation of nitric oxide signaling by formation of a distal receptor-ligand complex. [PDF]
, 2017 The binding of nitric oxide (NO) to the heme cofactor of heme-nitric oxide/oxygen binding (H-NOX) proteins can lead to the dissociation of the heme-ligating histidine residue and yield a five-coordinate nitrosyl complex, an important step for NO ...
Britt, R David +5 more
core +1 more source
Soluble Guanylate Cyclase [PDF]
Circulation, 2009 Arthur Kornberg, who shared with Severo Ochoa the 1959 Nobel Prize for Physiology or Medicine, stated in his autobiography that he had never met a dull enzyme.1 We strongly believe that soluble guanylate cyclase (sGC; E.C.4.6.1.2) is no exception to this.
John C. Burnett, Guido Boerrigter
openaire +3 more sources
GUCY2C maintains intestinal LGR5+ stem cells by opposing ER stress [PDF]
, 2017 Long-lived multipotent stem cells (ISCs) at the base of intestinal crypts adjust their phenotypes to accommodate normal maintenance and post-injury regeneration of the epithelium.
Kraft, Crystal +5 more
core +2 more sources
Recoverin Regulates Light-dependent Phosphodiesterase Activity in Retinal Rods [PDF]
, 2004 The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial.
Baylor, D. A. +6 more
core +5 more sources
Frontiers in Molecular Neuroscience, 2014 Atrial natriuretic factor receptor guanylate cyclase, ANF-RGC, was the first discovered member of the mammalian membrane guanylate cyclase family. The hallmark feature of the family is that a single protein contains both the site for recognition of the ...
Teresa eDuda +2 more
doaj +1 more source
FRET nanoscopy enables seamless imaging of molecular assemblies with sub-nanometer resolution [PDF]
arXiv, 2021 By circumventing the optical diffraction limit, super-resolved fluorescence microscopies enable the study of larger cellular structures and molecular assemblies. However, fluorescence nanoscopy currently lacks the spatiotemporal resolution to resolve distances on the size of individual molecules and reveal the conformational fine structure and dynamics
arxiv