FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Ars Iuris Salmanticensis, 2019 In the present work, we examine the origins of administrative guarantees, going back to France in the 18th century, and their counterpart in the Spanish legal order of the 19th century. In France, administrative guarantees are achieved in the hierarchical appeal, which becomes the appeal for abuse of power, together with the appeal for full ...
openaire +1 more source
New conditions for stability of multiple delayed Cohen-Grossberg Neural Networks of neutral-type. [PDF]
PLoS OneOzcan N.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Vehicle-Conditional Split-Conformal Calibration for Risk-Budgeted Sub-Second Proxy-Triggered Vehicle Instability Warnings from Past-Only Sensor Slices. [PDF]
Sensors (Basel)Yang J, Liu J, Tian K, Lin Y, Zhang J.
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Touch-based interaction dataset for user behavioral analysis in mobile devices. [PDF]
Data BriefGarabato D +5 more
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Intentional subintimal loop technique for orientation recovery in superficial femoral artery chronic total occlusion with pedal-origin controlled antegrade and retrograde subintimal tracking. [PDF]
J Vasc Surg Cases Innov TechKim TH, Kim JB, Jang HJ.
europepmc +1 more source