Results 191 to 200 of about 2,639,376 (307)

A CURE for synthetic regulation of gene expression: rapid screening of guide RNA efficacy as a framework for enabling undergraduate research in plant synthetic biology. [PDF]

Synth Biol (Oxf)
Bull T, Carlsen L, Hoglund N, Blarr J, Ciernia M, Daughtrey H, Gulnac K, Kathan Z, Labovitz B, Lonergan R, McDermott M, Medina A, Mikol Z, Miller Z, Prahl K, Rifai C, Schrems E, Shinkawa F, Summerfield J, Thevarajah E, Wagner S, Zimmerman T, Khakhar A.   +22 more
europepmc   +1 more source

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Dual-single-guide RNA strategy improves CRISPR-mediated homology-directed repair in Aspergillus. [PDF]

Nucleic Acids Res
Fu M, Wang J, Li J, Zhou Y, Huang X, Jia Z, Luo Y, Tan X, Gao Y, Yu B, Duan Y, Bu Q, Li X, Wang Y, Takaya N, Zhou S.   +15 more
europepmc   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

RAPID-DASH: fast and efficient assembly of guide RNA arrays for multiplexed CRISPR-Cas9 applications. [PDF]

Synth Biol (Oxf)
Salaudeen AL, Mateyko N, de Boer CG.
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Leveraging uncertainty quantification to optimize CRISPR guide RNA selection. [PDF]

Biol Methods Protoc
Schmitz C, Bradford J, Salomone R, Perrin D.   +3 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Variation in guide RNA library representation results in gene effect score bias in genome-wide CRISPR screens. [PDF]

BMC Genomics
Metz P, Alves-Vasconcelos S, Wallbank R, Riepsaame J, Brown S, Hassan AB.   +5 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source