Results 291 to 300 of about 4,450,920 (398)

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Single-guide RNA Cas9 and enhanced-deletion Cas9 rescue a recurrent <i>USH2A</i>-related splicing defect. [PDF]

Mol Ther Nucleic Acids
De Angeli P, Spaag S, Shliaga S, Flores-Tufiño A, Ritter M, Nasri M, Stingl K, Kühlewein L, Wissinger B, Kohl S.   +9 more
europepmc   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Model-directed generation of artificial CRISPR-Cas13a guide RNA sequences improves nucleic acid detection. [PDF]

Nat Biotechnol
Mantena S, Pillai PP, Petros BA, Welch NL, Myhrvold C, Sabeti PC, Metsky HC.   +6 more
europepmc   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Deep learning enhancing guide RNA design for CRISPR/Cas12a-based diagnostics. [PDF]

Imeta
Huang B, Guo L, Yin H, Wu Y, Zeng Z, Xu S, Lou Y, Ai Z, Zhang W, Kan X, Yu Q, Du S, Li C, Wu L, Huang X, Wang S, Wang X.   +16 more
europepmc   +1 more source

Additional file 5: of CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries

, 2016
Florian Heigwer, Tianzuo Zhan, Marco Breinig, Jan Winter, Dirk BrĂźgemann, Svenja Leible, Michael Boutros   +6 more
openalex   +1 more source

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

FEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah, Hui Li, Rushan Yan, Chengyu Chen, Madiha Islam, Abdul Majid, Ibrar Ahmed, Parviz Heidari, Xiaoxuan Tian   +8 more
wiley   +1 more source

Intelligent guide RNA: dual toehold switches for modulating luciferase in the presence of trigger RNA. [PDF]

Commun Biol
Hashemabadi M, Sasan HA, Hosseinkhani S, Amandadi M, Samareh Gholami A, Sadeghizadeh M.   +5 more
europepmc   +1 more source

CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants.

Molecular Plant, 2014
Yang Lei, Li Lu, Haiyang Liu, Sen Li, Feng Xing, Lingling Chen   +5 more
semanticscholar   +1 more source