Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
The Essence of Clinical Practice Guidelines for Lumbar Spinal Stenosis, 2021: 4. Surgical Treatment
Gen Inoue
doaj +1 more source
British Elbow and Shoulder Society practice guidelines: Rehabilitation following traumatic anterior shoulder dislocation (post-operative and non-operative care). [PDF]
Wong C, Jaggi A, Kearney R, Gwilym S.
europepmc +1 more source
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Routine psychosocial care in infertility and medically assisted reproduction – A guide for fertility staff: Summary [PDF]
ESHRE Psychology and Counselling Guideline Development Group +1 more
core
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
The Effects of Physical Activity Timing and Adherence to Physical Activity Guidelines on Sleep in Children With ADHD. [PDF]
Liang X +7 more
europepmc +1 more source
The impact of comorbidity and guideline-concordant care on survival in ovarian cancer patients
Jennifer S. Ferris +11 more
openalex +1 more source
A learning process towards person-centred care: A second-year follow-up of guideline implementation
K Kindblom +3 more
openalex +1 more source

