Regulation and Reversibility of Vacuolar H+-ATPase [PDF]
Arabidopsis thaliana vacuolar H(+)-translocating pyrophosphatase (V-PPase) was expressed functionally in yeast vacuoles with endogenous vacuolar H(+)-ATPase (V-ATPase), and the regulation and reversibility of V-ATPase were studied using these vacuoles. Analysis of electrochemical proton gradient (DeltamuH) formation with ATP and pyrophosphate indicated
T, Hirata +4 more
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Versatility of NaCl transport mechanisms in the cortical collecting duct [PDF]
Versatility of NaCl transport mechanisms in the cortical collecting duct. Am J Physiol Renal Physiol 313: F1254 –F1263, 2017. First published September 6, 2017; doi:10.1152/ajprenal.00369.2017.—The cortical collecting duct (CCD) forms part of the ...
Crambert, Gilles, Edwards, Aurelie
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Tetrahydrocarbazoles are a novel class of potent P-type ATPase inhibitors with antifungal activity. [PDF]
We have identified a series of tetrahydrocarbazoles as novel P-type ATPase inhibitors. Using a set of rationally designed analogues, we have analyzed their structure-activity relationship using functional assays, crystallographic data and computational ...
Maike Bublitz +10 more
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Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae [PDF]
In the budding yeast Saccharomyces cerevisiae, the Cdc42 effector Ste20 plays a crucial role in the regulation of filamentous growth, a response to nutrient limitation.
Höfken, T, Kane, PM, Li, SC, Lin, M
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The effect of inhibitors of plasma membrane oxidoreductases (quinacrine and dicumarol) and H+-ATPase (dicyclohexylcarbodiimide and orthovanadate) on ammonium uptake by Pisum arvense seedlings and the activities of H+-ATPase and NADH-ferricyanide ...
Genowefa Kubik-Dobosz +4 more
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The Role of the Plasma Membrane H+-ATPase in Plant Responses to Aluminum Toxicity
Aluminum (Al) toxicity is a key factor limiting plant growth and crop production on acid soils. Increasing the plant Al-detoxification capacity and/or breeding Al-resistant cultivars are a cost-effective strategy to support crop growth on acidic soils ...
Jiarong Zhang +10 more
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A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, although it is unclear whether host H+-ATPase activity and gene expression are involved in the physiological process.
Hui-Qian Cheng +4 more
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Influence of different iron availability on phosphoenolpiruvate carboxilase and malate dehydrogenase in roots of maize (Zea Mays L.) plants grown under iron deficiency [PDF]
The effect of the different nitrate availability on some enzymatic activities has been evaluated in iron deficient and iron sufficient maize plants (Zea mays L.).
Adalgisa Belligno, Marco Antonio Russo
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Measurement of ATP Hydrolytic Activity of Plasma Membrane H+-ATPase from Arabidopsis thaliana Leaves
Plant plasma membrane H+-ATPase, which is a P-type ATPase, couples ATP hydrolysis to H+ extrusion and thereby generates an electrochemical gradient across the plasma membrane. The proton gradient is necessary for secondary transport, cell elongation, and
Masaki Okumura, Toshinori Kinoshita
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Cryo-EM structures of intact V-ATPase from bovine brain
The vacuolar-type H+ -ATPases (V-ATPase) hydrolyze ATP to pump protons across the plasma or intracellular membrane. Here authors report two cryo-EM structures of the intact V-ATPase from bovine brain with all the subunits including the subunit H, which ...
Rong Wang +6 more
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