Results 291 to 300 of about 16,684 (344)

Cryopreservation of Transformed (Hairy) Roots ofArtemisia annua

Cryobiology, 1996
The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection.
K H, Teoh, P J, Weathers, R D, Cheetham, D B, Walcerz   +3 more
openaire   +2 more sources

Induction of hairy roots in Gmelina arborea Roxb. and production of verbascoside in hairy roots

Plant Science, 2005
Seedling tissues of Gmelina arborea, a medicinally important tree species, were infected with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 32% of the explants. Transgenic status of the roots was confirmed by PCR using rolB specific primers, and subsequently, by Southern analysis of the PCR ...
Shrutika Dhakulkar, T.R. Ganapathi, Sujata Bhargava, V.A. Bapat   +3 more
openaire   +1 more source

Hairy Root Cultures for Secondary Metabolites Production

2010
Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR disease leading to the neoplastic growth of roots that are characterized by high growth rate in hormone free media and genetic stability.
PISTELLI, LAURA, Giovannini, A., Ruffoni, B., BERTOLI, ALESSANDRA, PISTELLI, LUISA   +4 more
openaire   +2 more sources

Hairy root transformation in alfalfa (Medicago sativa L.)

Theoretical and Applied Genetics, 1987
The widely cultivated forage legume alfalfa (Medicago sativa L.) was transformed with the agropine type Agrobacterium rhizogenes NCPPB 1855. Sterile root and callus cultures were derived from tumorous hairy roots which were easily obtained independent of the plant variety or genotype.
Spanò L., Mariotti D., PEZZOTTI, Mario, Damiani F., Arcioni S.   +4 more
openaire   +4 more sources

Production and secretion of a heterologous protein by turnip hairy roots with superiority over tobacco hairy roots

Biotechnology Letters, 2013
A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were ...
Yoann, Huet, Jean-Pierre Ele, Ekouna, Aurore, Caron, Katiba, Mezreb, Michèle, Boitel-Conti, François, Guerineau   +5 more
openaire   +2 more sources

Elicitation of withaferin-A in hairy root culture of Withania somnifera (L.) Dunal using natural polysaccharides

Biologia, 2019
Withaferin-A (WA) is a major bioactive compound in the roots of Withania somnifera. It is reported to possess inhibitory effects against breast cancer, liver cancer, melanoma cancer and prostate cancer cells.
Chandrasekaran Thilip, Valiyaparambath Musfir Mehaboob, K. Varutharaju, K. Faizal, Palusamy Raja, A. Aslam, A. Shajahan   +6 more
semanticscholar   +1 more source

Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize

Science in China Series C: Life Sciences, 2006
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Hongwei, Xu, Xiaofu, Zhou, Jingmei, Lu, Junjie, Wang, Xingzhi, Wang   +4 more
openaire   +2 more sources

Large-Scale Production of Hairy Root

2004
Many products of interest are synthesized in organized tissues, but not formed in suspension or callus culture. Therefore, most attention has been focused on root cultures. The transgenic plant,"hairy root", has brought us to dramatic improvements in growth rate and high content of desirable products.
openaire   +2 more sources

Agrobacterium rhizogenes Mediated Hairy Root Cultures: A Promising Approach for Production of Useful Metabolites

New and Future Developments in Microbial Biotechnology and Bioengineering, 2019
The production of secondary metabolites through hairy root cultures has proven its importance by its escalating commercial relevance. In the present era hairy root cultures are good alternatives for the production of secondary metabolites, especially for
J. M. Rawat, A. Bhandari, Manoj Raturi, Balwant Rawat   +3 more
semanticscholar   +1 more source

Establishment of hairy root cultures of Rehmannia elata N.E. Brown ex Prain and production of iridoid and phenylethanoid glycosides

Industrial crops and products (Print), 2019
Hairy root lines were established through the infection of Agrobacterium rhizogenes strain (A4) from shoot tips and leaves of Rehmannia elata N. E. Brown ex Prain (Orobanchaceae).
E. Piątczak, A. Jeleń, J. Makowczyńska, S. Zielińska, Ł. Kuźma, E. Balcerczak   +5 more
semanticscholar   +1 more source