Results 291 to 300 of about 1,132,099 (329)
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Plant Molecular Biology, 2005
Activation tagging is a powerful technique for generating gain-of-function mutants in plants. We developed a new vector system for activation tagging of genes in "transformed hairy roots". The binary vector pHR-AT (Hairy Root-Activation Tagging) and its derivative pHR-AT-GFP contain a cluster of rol (rooting locus) genes together with the right border ...
Yasuo Niwa +5 more
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Activation tagging is a powerful technique for generating gain-of-function mutants in plants. We developed a new vector system for activation tagging of genes in "transformed hairy roots". The binary vector pHR-AT (Hairy Root-Activation Tagging) and its derivative pHR-AT-GFP contain a cluster of rol (rooting locus) genes together with the right border ...
Yasuo Niwa +5 more
openaire +2 more sources
Progress and Prospects of Hairy Root Research
2018Nature’s own genetic engineer Agrobacterium rhizogenes was discovered more than 40 years ago, and an increasing number of publications on the use of hairy roots in biotechnology have been published since – with more than 85% of all the publications during the past 15 years. Hairy roots have been successfully exploited in various fields in biotechnology,
Suvi T. Häkkinen +1 more
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Science in China Series C: Life Sciences, 2006
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Xingzhi Wang +4 more
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Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Xingzhi Wang +4 more
openaire +3 more sources
Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens
Biotechnology & Bioengineering, 2000Hairy roots were used to investigate cadmium uptake by Thlaspi caerulescens, a metal hyperaccumulator plant with potential applications in phytoremediation and phytomining. Experiments were carried out in nutrient media under conditions supporting root growth.
Tatjana V. Nedelkoska, Pauline M. Doran
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Establishment of hairy root cultures of Psoralea species
Plant Cell Reports, 1992Eight Psoralea species (Leguminosae) were inoculated with Agrobacterium rhizogenes, strains 8196 and 9402. Hairy roots were only induced by strain 9402. Attention was focussed on Psoralea lachnostachys. Transformed roots grew very rapidly in Gamborg B5 liquid medium with a doubling time of the culture of 38 hours.
Nguyen, Christophe +3 more
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The Journal of the Science of Food and Agriculture, 2019
BACKGROUND Dracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as, xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects as well as, antiproliferative potential against ...
E. Nourozi +3 more
semanticscholar +1 more source
BACKGROUND Dracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as, xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects as well as, antiproliferative potential against ...
E. Nourozi +3 more
semanticscholar +1 more source
Chinese Journal of Biotechnology, 2006
The effects of exogenous phytohormones on hairy root growth and biosynthesis of anthraquinones in the hairy root cultures of Polygonum multiflorum Thunb. were studied. The results showed that the 2,4-D, NAA and 6-BA all have obvious effects on the growth of hairy root cultures and the biosynthesis of anthraquinones.
Na Ma, Rong-Min Yu, Chunyan Yan, Yu Zhao
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The effects of exogenous phytohormones on hairy root growth and biosynthesis of anthraquinones in the hairy root cultures of Polygonum multiflorum Thunb. were studied. The results showed that the 2,4-D, NAA and 6-BA all have obvious effects on the growth of hairy root cultures and the biosynthesis of anthraquinones.
Na Ma, Rong-Min Yu, Chunyan Yan, Yu Zhao
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Hairy Roots as a Source of Tropane Alkaloids [PDF]
, 2020 The hairy roots developed after Agrobacterium rhizogenes transformation can be used for the production of secondary metabolites which show medicinal properties. Plant secondary metabolites include various classes of chemical compounds, such as the tropane alkaloids found in some members of the Solanaceae family, for example, Atropa belladonna, Datura ...
Przemysław Sitarek, Ewa Skała
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Biologia, 2019
Withaferin-A (WA) is a major bioactive compound in the roots of Withania somnifera. It is reported to possess inhibitory effects against breast cancer, liver cancer, melanoma cancer and prostate cancer cells.
Chandrasekaran Thilip +6 more
semanticscholar +1 more source
Withaferin-A (WA) is a major bioactive compound in the roots of Withania somnifera. It is reported to possess inhibitory effects against breast cancer, liver cancer, melanoma cancer and prostate cancer cells.
Chandrasekaran Thilip +6 more
semanticscholar +1 more source
Production of monoclonal antibodies by tobacco hairy roots
Biotechnology and Bioengineering, 1997Hairy roots of tobacco (Nicotiana tabacum) were used to produce full-length murine lgG(1) monoclonal antibody. The presence of heavy (gamma) and light (kappa) chains and fully assembled antibody was verified by Western blot analysis of root extracts. Antibody levels in the biomass and medium were quantified by ELISA based on detection of gamma-kappa ...
Pauline M. Doran, Raviwan Wongsamuth
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