Results 41 to 50 of about 16,684 (344)

Transformation and Detection of Soybean Hairy Roots

BIO-PROTOCOL, 2023
Agrobacterium rhizogenes is a soil bacteria with extensive infectivity, which can infect almost all dicotyledonous plants and a few monocotyledonous plants to induce root nodules. This is caused by the root-inducing plasmid, which contains genes responsible for the autonomous growth of root nodules and crown gall base synthesis.
Xing Xu, Tai- Yu, Jian Ma, Jun Chen, Yong- Zhou, Ming Chen, Zhan- Chen, You- Ma, Zhao- Xu, Zhi- Zhang   +9 more
openaire   +3 more sources

Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin

Brazilian Journal of Pharmaceutical Sciences, 2020
Hypericum sinaicum L. is an endangered Egyptian medicinal plant of high importance due to the presence of naphthodianthrones (hypericins), which have photodynamic properties and pharmaceutical potential. We sought to assess H. sinaicum ability to develop
Heba Desouky Khlifa, Magdalena Klimek-Chodacka, Rafal Baranski, Michal Combik, Hussein Sayed Taha   +4 more
doaj   +1 more source

First‐generation genome editing in potato using hairy root transformation

Plant Biotechnology Journal, 2020
Summary Genome editing and cis‐gene breeding have rapidly accelerated crop improvement efforts, but their impacts are limited by the number of species capable of being genetically transformed.
Nathaniel M. Butler, S. Jansky, Jiming Jiang   +2 more
semanticscholar   +1 more source

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Acta Agriculturae Slovenica, 2016
Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A.
Elnaz NOUROZI, Bahman HOSSEINI, Abbas HASSANI   +2 more
doaj   +1 more source

Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter

Plant Methods, 2022
Background Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes.
Jia-Wen Li, Tuo Zeng, Zhi-Zhuo Xu, Jin-Jin Li, Hao Hu, Qin Yu, Li Zhou, Ri-Ru Zheng, Jing Luo, Cai-Yun Wang   +9 more
doaj   +1 more source

Efficient Generation of CRISPR/Cas9-Mediated Homozygous/Biallelic Medicago truncatula Mutants Using a Hairy Root System

Frontiers in Plant Science, 2020
In the process of acquiring mutants mediated by CRISPR/Cas9, plantlets are often regenerated from both mutated and non-mutated cells in a random manner, which increase the odds of chimeric mutated plant. In general, it’s necessary to infect more explants
Hailing Zhang, Yingping Cao, Huan Zhang, Yue Xu, Chuanen Zhou, Wenwen Liu, R. Zhu, Chen Shang, Jikai Li, Zhongbao Shen, Siyi Guo, Zhubing Hu, C. Fu, Dequan Sun   +13 more
semanticscholar   +1 more source

Transcriptomic Analysis Reveals That Reactive Oxygen Species and Genes Encoding Lipid Transfer Protein Are Associated with Tobacco Hairy Root Growth and Branch Development

Molecular Plant-Microbe Interactions, 2014
The hairy root, a specialized plant tissue that emerges from a cell transformed with transfer DNA (T-DNA) from Agrobacterium rhizogenes, can be used to study root biology and utilized in biotechnological applications.
Jung-Hao Wang, Hsiao-Han Lin, Chi-Te Liu, Ta-Chung Lin, Li-yu Daisy Liu, Kung-Ta Lee   +5 more
doaj   +1 more source

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Plant Methods, 2023
Background The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family.
Huiqing Yan, Dandan Ma, Peipei Yi, Guilian Sun, Xingyan Chen, Yin Yi, Xiaolong Huang   +6 more
doaj   +1 more source

Production of Prenylated Stilbenoids in Hairy Root Cultures of Peanut (Arachis hypogaea) and Its Wild Relatives A. ipaensis and A. duranensis via an Optimized Elicitation Procedure

Molecules, 2020
Prenylated stilbenoids are phenolic compounds produced in a small number of plants such as peanut (Arachis hypogaea) to counteract biotic and abiotic stresses.
Lingling Fang, Tianhong Yang, F. Medina-Bolivar   +2 more
semanticscholar   +1 more source

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Acta Agriculturae Slovenica, 2014
Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V.
Ali PAKDIN PARIZI, Mohammad FARSI, Ghorban-Ali NEMATZADEH, Amin MIRSHAMSI   +3 more
doaj   +1 more source