Results 121 to 130 of about 5,583 (160)

Iron-uptake in the Euryarchaeon Halobacterium salinarum

BioMetals, 2007
Iron-uptake is well studied in a plethora of pro- and eukaryotic organisms with the exception of Archaea, which thrive mainly in extreme environments. In this study, the mechanism of iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum strain JW 5 was analyzed.
Dirk, Hubmacher, Berthold F, Matzanke, Stefan, Anemüller   +2 more
openaire   +2 more sources

Repair of UV damage in Halobacterium salinarum

Biochemical Society Transactions, 2003
Halobacterium is one of the few known Archaea that tolerates high levels of sunlight in its natural environment. Photoreactivation is probably its most important strategy for surviving UV irradiation and we have shown that both of the major UV photoproducts, cyclobutane pyrimidine dimers (CPDs) and (6–4) photoproducts, can be very efficiently repaired ...
S, McCready, L, Marcello
openaire   +2 more sources

The DpsA-homologue of the archaeon Halobacterium salinarum is a ferritin

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2002
An iron-rich protein, DpsA(Hsal), was isolated from the archaeon Halobacterium salinarum sharing a sequence identity of 35% with the starvation-induced DNA-binding protein, DpsA, of Synechecoccus sp. PCC7942. It consists of 20-kDa subunits forming a dodecameric structure.
Sabine, Reindel, Stefan, Anemüller, Andrzej, Sawaryn, Berthold F, Matzanke   +3 more
openaire   +2 more sources

Investigations of iron uptake in Halobacterium salinarum

Biochemical Society Transactions, 2002
The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake of 55Fe and [14C]citrate gave evidence only for the accumulation of iron.
D, Hubmacher, B F, Matzanke, S, Anemüller   +2 more
openaire   +2 more sources

The Low Molecular Weight Proteome of Halobacterium salinarum

Journal of Proteome Research, 2007
Systematic investigation of low molecular weight proteins (LMW, below 20 kDa) in the archaeon Halobacterium salinarum resulted in a 6-fold enhancement of the identification rate, reaching 35% of the theoretical proteome in that size range. This was achieved by optimization of common protocols for protein analysis with general applicability.
Klein, C., Aivaliotis, M., Olsen, J., Falb, M., Besir, H., Scheffer, B., Bisle, B., Tebbe, A., Konstantinidis, K., Siedler, F., Pfeiffer, F., Mann, M., Oesterhelt, D.   +12 more
openaire   +3 more sources

Isotopic Labeling of Proteins in Halobacterium salinarum

2015
It is often necessary to obtain isotopically labeled proteins containing (15)N, (13)C, or (2)H for nuclear magnetic resonance; and (2)H for small-angle neutron scattering or neutron diffraction studies. To achieve uniform isotopic labeling, protein expression is most commonly performed in Escherichia coli or yeast using labeled media. However, proteins
Thomas E, Cleveland, Zvi, Kelman
openaire   +2 more sources

On the multicomponent nature of Halobacterium salinarum flagella

Microbiology, 2007
Filaments of the flagellum of the halophilic archaeon Halobacterium salinarum consist of five flagellins: A1, A2, B1, B2, and B3, which are encoded by five genes localized in tandem in two flgA and flgB operons. While the role of flagellins A1 and A2 has been determined, the role of the proteins, B operon products, is still unclear.
S. N. Beznosov, M. G. Pyatibratov, O. V. Fedorov   +2 more
openaire   +1 more source

Effects of divalent cations on Halobacterium salinarum cell aggregation

Journal of Bioscience and Bioengineering, 2007
Ca(2+) was found to be essential for initiating Halobacterium salinarum CCM 2090 cell aggregation. The floc formed from such aggregation could easily be dissociated without cellular lysis by sodium citrate. Cr(2+), Mn(2+), Fe(3+), Co(2+), Ni(2+), Cu(2+), and Zn(2+) could replace Ca(2+). However, Mg(2+), Sr(2+), Mo(2+), Cd(2+), Sn(2+), Hg(2+), and Pb(2+)
Yoshitaka, Kawakami, Nobuhiro, Hayashi, Mizue, Ema, Masashi, Nakayama   +3 more
openaire   +2 more sources

Proteomanalysen an Halobacterium salinarum

2005
Im Rahmen dieser Arbeit wurden verschiedene Proteome von H. salinarum untersucht, die nach zellulären Kompartimenten unterschieden wurden in (1) das Flagellarmotor-Proteom (2) das Cytosolproteom und (3) das Membranproteom. Die Untersuchung des Flagellarmotors erfolgte hauptsächlich auf struktureller Basis mittels Elektronenmikroskopie.
openaire   +2 more sources

Flagellar Rotation in the Archaeon Halobacterium salinarum Depends on ATP

Journal of Molecular Biology, 2008
Halobacterium salinarum swims with the help of a polarly inserted flagellar bundle. In energized cells, the flagellar motors rotate continuously, occasionally switching the rotational sense. Starving cells become immotile as the energy level drops. Presumably, there is a threshold of energy required for flagellar rotation.
Streif, S., Staudinger, W., Marwan, W., Oesterhelt, D.   +3 more
openaire   +4 more sources