The Adaptive Immune System of Haloferax volcanii [PDF]
To fight off invading genetic elements, prokaryotes have developed an elaborate defence system that is both adaptable and heritable—the CRISPR-Cas system (CRISPR is short for: clustered regularly interspaced short palindromic repeats and Cas: CRISPR ...
Lisa-Katharina Maier +2 more
doaj +3 more sources
Extracellular DNA metabolism in Haloferax volcanii [PDF]
Extracellular DNA is found in all environments and is a dynamic component of the micro-bial ecosystem. Microbial cells produce and interact with extracellular DNA through many endogenous mechanisms.
Scott eChimileski +4 more
doaj +3 more sources
Chase Assay of Protein Stability in Haloferax volcanii
Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study ...
Xian Fu, Julie Maupin-Furlow
doaj +4 more sources
Dihydroxyacetone metabolism in Haloferax volcanii [PDF]
Dihydroxyacetone (DHA) is a ketose sugar that can be produced by oxidizing glycerol. DHA in the environment is taken up and phosphorylated to DHA-phosphate by glycerol kinase or DHA kinase.
Matthew eOuellette +2 more
doaj +3 more sources
Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei [PDF]
Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-point chromosomal comparison revealed
P, López-García +3 more
openaire +2 more sources
Regulatory RNAs in Haloferax volcanii [PDF]
In organisms of all three domains of life, a plethora of sRNAs (small regulatory RNAs) exists in addition to the well-known RNAs such as rRNAs, tRNAs and mRNAs. Although sRNAs have been well studied in eukaryotes and in bacteria, the sRNA population in archaea has just recently been identified and only in a few archaeal species.
Susan, Fischer +12 more
+10 more sources
Translationsinitiationsfaktoren in Haloferax volcanii [PDF]
In allen drei Domänen des Lebens ist in der Translation die Initiation der geschwindigkeits-bestimmende Schritt. Die Effizienz der Translationsinitiation und ihre unterschiedliche Regula-tion ist von Translationsinitiationsfaktoren (IFs) abhängig. Bakterien enthalten nur drei IFs, während die Anzahl bei Archaeen (aIFs) und Eukaryoten (eIFs) deutlich ...
openaire +2 more sources
Bergey's Manual of Systematics of Archaea and Bacteria (BMSAB) is a reference work aimed at undergraduates, graduate students, researchers, professors and experienced professionals at all levels. About a hundred new genera and 600+ new species have been described per year for each of the last 5 years.
Aharon Oren
wiley +2 more sources
An expression vector for the archaebacterium Haloferax volcanii [PDF]
The recent development of an efficient transformation method and shuttle vectors for Haloferax volcanii has set the stage for rapid progress in archaebacterial molecular biology. We describe a shuttle-expression vector that can be selected for and maintained in either H.
D T, Nieuwlandt, C J, Daniels
openaire +2 more sources
N-glycosylation in Haloferax volcanii: adjusting the sweetness [PDF]
Long believed to be restricted to Eukarya, it is now known that cells of all three domains of life perform N-glycosylation, the covalent attachment of glycans to select target protein asparagine residues. Still, it is only in the last decade that pathways of N-glycosylation in Archaea have been delineated.
Eichler, Jerry +5 more
openaire +3 more sources

