The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the β7-β8 and β9-β10 hairpin loops and conserved zinc binding domain cysteines [PDF]
The hexameric MCM complex is the catalytic core of the replicative helicase in eukaryotic and archaeal cells. Here we describe the first in vivo analysis of archaeal MCM protein structure and function relationships using the genetically tractable ...
Tatjana P Kristensen +13 more
core +1 more source
Primary structure and glycosylation of the S-layer protein of Haloferax volcanii [PDF]
The outer surface of the archaebacterium Haloferax volcanii (formerly named Halobacterium volcanii) is covered with a hexagonally packed surface (S) layer. The gene coding for the S-layer protein was cloned and sequenced.
Sumper, Manfred +3 more
core +1 more source
A bioluminescent reporter for the halophilic archaeon Haloferax volcanii
Haloarchaea have evolved to thrive in hypersaline environments. Haloferax volcanii is of particular interest due to its genetic tractability; however, few in vivo reporters exist for halophiles. Haloarchaeal proteins evolved characteristics that promote proper folding and function at high salt concentrations, but many mesophilic reporter proteins lack ...
Chris R. Davis +2 more
openaire +3 more sources
While archaeal proteomics advanced rapidly, a comprehensive proteome database for archaea is lacking. Therefore, the authors here launch the Archaeal Proteome Project, a community-effort providing insights into archaeal cell biology via the combined ...
Stefan Schulze +18 more
doaj +1 more source
Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism [PDF]
This work is supported by the The Scottish Universities Life Sciences Alliance (SULSA)Single-stranded DNA-binding proteins (SSBs) play vital roles in all aspects of DNA metabolism in all three domains of life and are characterized by the presence of one ...
Skowyra, Agnieszka +4 more
core +1 more source
tRNA-like elements in Haloferax volcanii
All functional RNAs are generated from precursor molecules by a plethora of processing steps. The generation of mature RNA molecules by processing is an important layer of gene expression regulation catalysed by ribonucleases. Here, we analysed 5S rRNA processing in the halophilic Archaeon Haloferax volcanii. Earlier experiments showed that the 5S rRNA
Hölzle, Annette +5 more
openaire +2 more sources
Haloferax volcanii theoretical proteome exported from HaloLex
The theoretical proteome is derived from the annotated Haloferax volcanii DS2 genome (Hartmann et al, 2010). This proteome represents a Gold Standard Protein based annotation (Pfeiffer and Oesterhelt, 2015).
Pfeiffer, Friedhelm +1 more
core +3 more sources
Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea : research article [PDF]
Background Differential expression of genes can be regulated on many different levels. Most global studies of gene regulation concentrate on transcript level regulation, and very few global analyses of differential translational efficiencies exist.
Schuster Stephan C +31 more
core +1 more source
The ability to form biofilms is shared by many microorganisms, including archaea. Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while ...
Heather Schiller +8 more
doaj +1 more source
parameter and coordinate files to start an MD simulation of Haloferax volcanii ADH2
<p>parameter and coordinate files to start an MD simulation of Haloferax volcanii ADH2 in Amber</p>halophilic proteins ionic ...
Schindl, Alexandra
core +1 more source

