Differences in homologous recombination and maintenance of heteropolyploidy between Haloferax volcanii and Haloferax mediterranei. [PDF]
G3 (Bethesda), 2023 Abstract Polyploidy, the phenomenon of having more than one copy of the genome in an organism, is common among haloarchaea. While providing short-term benefits for DNA repair, polyploidy is generally regarded as an “evolutionary trap” that by the notion of the Muller's ratchet will inevitably conclude in the species' decline or even ...
Dattani A +6 more
europepmc +10 more sources
Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei [PDF]
Journal of Bacteriology, 1995 Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-point chromosomal comparison revealed
P, López-García +3 more
openaire +2 more sources
Motile ghosts of the halophilic archaeon, Haloferax volcanii. [PDF]
Proc Natl Acad Sci U S A, 2020 Significance Molecular motors are natural molecular machines that convert chemical energy to directional mechanical motion (e.g., ATP [adenosine-5′-triphosphate]-driven linear motors myosin, kinesin, and dynein in Eukaryotes; the ion-coupled rotary bacterial flagellar motor in Bacteria). Reconstituted systems such as in vitro motility
Kinosita Y +8 more
europepmc +7 more sources
An expression vector for the archaebacterium Haloferax volcanii [PDF]
Journal of Bacteriology, 1990 The recent development of an efficient transformation method and shuttle vectors for Haloferax volcanii has set the stage for rapid progress in archaebacterial molecular biology. We describe a shuttle-expression vector that can be selected for and maintained in either H.
D T, Nieuwlandt, C J, Daniels
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Functional genomic and advanced genetic studies reveal novel insights into the metabolism, regulation, and biology of Haloferax volcanii [PDF]
, 2011 The genome sequence of Haloferax volcanii is available and several comparative genomic in silico studies were performed that yielded novel insight for example into protein export, RNA modifications, small non-coding RNAs, and ubiquitin-like Small ...
Jörg Soppa, Soppa, Jörg
core +1 more source
A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants [PDF]
, 2013 Translation is an important step in gene expression. The initiation of translation is phylogenetically diverse, since currently five different initiation mechanisms are known.
Jörg Soppa +14 more
core +1 more source
Mechanosensitive Ion Channels of the Archaeon Haloferax volcanii [PDF]
Journal of Biological Chemistry, 1998 Mechanosensitive (MS) ion channels have been documented in a variety of cells belonging to Eukarya and Eubacteria. We report the novel finding of two types of MS ion channels in the cell membrane of the halophilic archaeon Haloferax volcanii, a member of the Archaea that comprise the third phylogenetic domain.
A C, Le Dain +4 more
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The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the β7-β8 and β9-β10 hairpin loops and conserved zinc binding domain cysteines [PDF]
, 2014 The hexameric MCM complex is the catalytic core of the replicative helicase in eukaryotic and archaeal cells. Here we describe the first in vivo analysis of archaeal MCM protein structure and function relationships using the genetically tractable ...
Tatjana P Kristensen +12 more
core +1 more source
Haloferax volcanii for biotechnology applications: challenges, current state and perspectives [PDF]
, 2020 Haloferax volcanii is an obligate halophilic archaeon with its origin in the Dead Sea. Simple laboratory culture conditions and a wide range of genetic tools have made it a model organism for studying haloarchaeal cell biology.
Paradisi, Francesca +5 more
core +3 more sources
Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism [PDF]
, 2011 This work is supported by the The Scottish Universities Life Sciences Alliance (SULSA)Single-stranded DNA-binding proteins (SSBs) play vital roles in all aspects of DNA metabolism in all three domains of life and are characterized by the presence of one ...
Skowyra, Agnieszka +3 more
core +1 more source