Results 41 to 50 of about 5,137 (188)

Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea [PDF]

open access: yes, 2013
Gene homologs of GlnK PII regulators and AmtB-type ammonium transporters are often paired on prokaryotic genomes, suggesting these proteins share an ancient functional relationship.
Adler   +53 more
core   +2 more sources

tRNA-like elements in Haloferax volcanii

open access: yesBiochimie, 2012
All functional RNAs are generated from precursor molecules by a plethora of processing steps. The generation of mature RNA molecules by processing is an important layer of gene expression regulation catalysed by ribonucleases. Here, we analysed 5S rRNA processing in the halophilic Archaeon Haloferax volcanii. Earlier experiments showed that the 5S rRNA
Hölzle, Annette   +5 more
openaire   +2 more sources

DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii [PDF]

open access: yes, 2012
Halophilic archaea maintain intracellular salt concentrations close to saturation to survive in high-salt environments and their cellular processes have adapted to function under these conditions.
Bunting, KA, Patoli, B, Winter, JA
core   +2 more sources

Kleine Zinkfinger-Proteine in Haloferax volcanii [PDF]

open access: yes, 2021
In der vorliegenden Arbeit wurde das Zinkfinger-µ-Protein HVO_2753 des halophilen Archaeons Haloferax volcanii hinsichtlich seiner biologischen Funktion und seiner Struktur charakterisiert. Zinkfinger-µ-Proteine wurden bisher nur sehr wenig untersucht, während ihnen jedoch in den letzten Jahren steigendes Interesse entgegengebracht wird. Im Genom von H.
openaire   +3 more sources

An expression vector for the archaebacterium Haloferax volcanii [PDF]

open access: yesJournal of Bacteriology, 1990
The recent development of an efficient transformation method and shuttle vectors for Haloferax volcanii has set the stage for rapid progress in archaebacterial molecular biology. We describe a shuttle-expression vector that can be selected for and maintained in either H.
D T, Nieuwlandt, C J, Daniels
openaire   +2 more sources

Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii [PDF]

open access: yes, 2012
Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded β-sheet coiled into a closed barrel, to bind to ssDNA thereby protecting and ...
Allers, Thorsten   +2 more
core   +1 more source

A bioluminescent reporter for the halophilic archaeon Haloferax volcanii. [PDF]

open access: yesExtremophiles, 2020
Haloarchaea have evolved to thrive in hypersaline environments. Haloferax volcanii is of particular interest due to its genetic tractability; however, few in vivo reporters exist for halophiles. Haloarchaeal proteins evolved characteristics that promote proper folding and function at high salt concentrations, but many mesophilic reporter proteins lack ...
Davis CR, Johnson CH, Robertson JB.
europepmc   +4 more sources

Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation

open access: yesmSphere, 2020
The ability to form biofilms is shared by many microorganisms, including archaea. Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while ...
Heather Schiller   +8 more
doaj   +1 more source

Inhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii [PDF]

open access: yes, 2015
DNA ligases play an essential role in many aspects of DNA metabolism in all three domains of life. The haloarchaeal organism Haloferax volcanii encodes both ATP- and NAD+-dependent DNA ligase enzymes designated LigA and LigN, respectively.
Giroux, Xavier, MacNeill, Stuart
core   +2 more sources

Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo [PDF]

open access: yes, 2009
Recently a first genome-wide analysis of translational regulation using prokaryotic species had been performed which revealed that regulation of translational efficiency plays an important role in haloarchaea.
Mariam Brenneis   +43 more
core   +1 more source

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