Results 21 to 30 of about 5,166 (196)

Genetic and physical mapping of DNA replication origins in Haloferax volcanii. [PDF]

PLoS Genetics, 2007
The halophilic archaeon Haloferax volcanii has a multireplicon genome, consisting of a main chromosome, three secondary chromosomes, and a plasmid. Genes for the initiator protein Cdc6/Orc1, which are commonly located adjacent to archaeal origins of DNA ...
Cédric Norais, Michelle Hawkins, Amber L Hartman, Jonathan A Eisen, Hannu Myllykallio, Thorsten Allers   +5 more
doaj   +7 more sources

Glucose Metabolism and Acetate Switch in Archaea: the Enzymes in Haloferax volcanii [PDF]

Journal of Bacteriology, 2021
Marius Ortjohann, Ulrike Johnsen, Peter Schönheit   +2 more
exaly   +2 more sources

Quantitative Mass Spectrometry by SILAC in Haloferax volcanii. [PDF]

Methods Mol Biol, 2022
The development of mass spectrometry (MS)-based proteomics methods has been critical in providing new insight about cellular processes and adaptations in all domains of life. While traditional MS-based methods are not inherently quantitative, technologies are now available to overcome this limitation.
Couto-Rodriguez RL, Gal D, McMillan LJ, Koh J, Chen S, Maupin-Furlow JA.   +5 more
europepmc   +3 more sources

Improved growth and morphological plasticity of Haloferax volcanii. [PDF]

Microbiology (Reading), 2021
Abstract Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt to changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped (‘plate’) cells in culture, but may ...
de Silva RT, Abdul-Halim MF, Pittrich DA, Brown HJ, Pohlschroder M, Duggin IG.   +5 more
europepmc   +5 more sources

Differences in homologous recombination and maintenance of heteropolyploidy between Haloferax volcanii and Haloferax mediterranei. [PDF]

G3 (Bethesda), 2023
Abstract Polyploidy, the phenomenon of having more than one copy of the genome in an organism, is common among haloarchaea. While providing short-term benefits for DNA repair, polyploidy is generally regarded as an “evolutionary trap” that by the notion of the Muller's ratchet will inevitably conclude in the species' decline or even ...
Dattani A, Sharon I, Shtifman-Segal E, Robinzon S, Gophna U, Allers T, Altman-Price N.   +6 more
europepmc   +10 more sources

Cellular and Genomic Properties of Haloferax gibbonsii LR2-5, the Host of Euryarchaeal Virus HFTV1

Frontiers in Microbiology, 2021
Hypersaline environments are the source of many viruses infecting different species of halophilic euryarchaea. Information on infection mechanisms of archaeal viruses is scarce, due to the lack of genetically accessible virus–host models. Recently, a new
Colin Tittes, Sabine Schwarzer, Friedhelm Pfeiffer, Mike Dyall-Smith, Mike Dyall-Smith, Marta Rodriguez-Franco, Hanna M. Oksanen, Tessa E. F. Quax   +7 more
doaj   +1 more source

Motile ghosts of the halophilic archaeon, Haloferax volcanii. [PDF]

Proc Natl Acad Sci U S A, 2020
Significance Molecular motors are natural molecular machines that convert chemical energy to directional mechanical motion (e.g., ATP [adenosine-5′-triphosphate]-driven linear motors myosin, kinesin, and dynein in Eukaryotes; the ion-coupled rotary bacterial flagellar motor in Bacteria). Reconstituted systems such as in vitro motility
Kinosita Y, Mikami N, Li Z, Braun F, Quax TEF, van der Does C, Ishmukhametov R, Albers SV, Berry RM.   +8 more
europepmc   +7 more sources

A Small RNA Is Linking CRISPR–Cas and Zinc Transport

Frontiers in Molecular Biosciences, 2021
The function and mode of action of small regulatory RNAs is currently still understudied in archaea. In the halophilic archaeon Haloferax volcanii, a plethora of sRNAs have been identified; however, in-depth functional analysis is missing for most of ...
Pascal Märkle, Lisa-Katharina Maier, Sandra Maaß, Claudia Hirschfeld, Jürgen Bartel, Dörte Becher, Björn Voß, Anita Marchfelder   +7 more
doaj   +1 more source

Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei [PDF]

Journal of Bacteriology, 1995
Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-point chromosomal comparison revealed
P, López-García, A, St Jean, R, Amils, R L, Charlebois   +3 more
openaire   +2 more sources

An expression vector for the archaebacterium Haloferax volcanii [PDF]

Journal of Bacteriology, 1990
The recent development of an efficient transformation method and shuttle vectors for Haloferax volcanii has set the stage for rapid progress in archaebacterial molecular biology. We describe a shuttle-expression vector that can be selected for and maintained in either H.
D T, Nieuwlandt, C J, Daniels
openaire   +2 more sources