Results 21 to 30 of about 5,137 (188)

Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring

Bio-Protocol, 2022
The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions.
Paula Mondragon, Sungmin Hwang, Amy Schmid, Julie Maupin-Furlow   +3 more
doaj   +2 more sources

Investigation of the global translational response to oxidative stress in the model archaeon Haloferax volcanii reveals untranslated small RNAs with ribosome occupancy [PDF]

mSphere
Oxidative stress induces a wide range of cellular damage, often causing disease and cell death. While many organisms are susceptible to the effects of oxidative stress, haloarchaea have adapted to be highly resistant.
Emma Dallon, Haley M. Moran, Sadhana R. Chidambaran, Arman Kian, Betty Y. H. Huang, Stephen D. Fried, Jocelyne DiRuggiero   +6 more
doaj   +2 more sources

Iron starvation results in up-regulation of a probable Haloferax volcanii siderophore transporter [PDF]

Frontiers in Microbiology
The response of the haloarchaeal model organism Haloferax volcanii to iron starvation was analyzed at the proteome level by data-independent acquisition mass spectrometry. Cells grown in minimal medium with normal iron levels were compared to those grown
Anna-Lena Sailer, Zivojin Jevtic, Zivojin Jevtic, Britta Stoll, Julia Wörtz, Kundan Sharma, Henning Urlaub, Henning Urlaub, Mike Dyall-Smith, Mike Dyall-Smith, Friedhelm Pfeiffer, Friedhelm Pfeiffer, Anita Marchfelder, Christof Lenz, Christof Lenz   +14 more
doaj   +2 more sources

Improved growth and morphological plasticity of Haloferax volcanii. [PDF]

Microbiology (Reading), 2021
AbstractSome microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt to changed environmental conditions. The pleomorphic archaeonHaloferax volcaniicommonly forms discoid-shaped (‘plate’) cells in culture, but may also be present as rods, and can develop into motile rods in soft agar, or ...
de Silva RT, Abdul-Halim MF, Pittrich DA, Brown HJ, Pohlschroder M, Duggin IG.   +5 more
europepmc   +5 more sources

Regulatory RNAs in Haloferax volcanii [PDF]

Biochemical Society Transactions, 2011
In organisms of all three domains of life, a plethora of sRNAs (small regulatory RNAs) exists in addition to the well-known RNAs such as rRNAs, tRNAs and mRNAs. Although sRNAs have been well studied in eukaryotes and in bacteria, the sRNA population in archaea has just recently been identified and only in a few archaeal species.
Susan, Fischer, Juliane, Benz, Bettina, Späth, Angelika, Jellen-Ritter, Ruth, Heyer, Marcella, Dörr, Lisa-Katharina, Maier, Clemens, Menzel-Hobeck, Matthias, Lehr, Katharina, Jantzer, Julia, Babski, Jörg, Soppa, Anita, Marchfelder   +12 more
  +9 more sources

Quantitative Mass Spectrometry by SILAC in Haloferax volcanii. [PDF]

Methods Mol Biol, 2022
The development of mass spectrometry (MS)-based proteomics methods has been critical in providing new insight about cellular processes and adaptations in all domains of life. While traditional MS-based methods are not inherently quantitative, technologies are now available to overcome this limitation.
Couto-Rodriguez RL, Gal D, McMillan LJ, Koh J, Chen S, Maupin-Furlow JA.   +5 more
europepmc   +3 more sources

Motile ghosts of the halophilic archaeon, Haloferax volcanii. [PDF]

Proc Natl Acad Sci U S A, 2020
Significance Molecular motors are natural molecular machines that convert chemical energy to directional mechanical motion (e.g., ATP [adenosine-5′-triphosphate]-driven linear motors myosin, kinesin, and dynein in Eukaryotes; the ion-coupled rotary bacterial flagellar motor in Bacteria). Reconstituted systems such as in vitro motility
Kinosita Y, Mikami N, Li Z, Braun F, Quax TEF, van der Does C, Ishmukhametov R, Albers SV, Berry RM.   +8 more
europepmc   +7 more sources

Cellular and Genomic Properties of Haloferax gibbonsii LR2-5, the Host of Euryarchaeal Virus HFTV1

Frontiers in Microbiology, 2021
Hypersaline environments are the source of many viruses infecting different species of halophilic euryarchaea. Information on infection mechanisms of archaeal viruses is scarce, due to the lack of genetically accessible virus–host models. Recently, a new
Colin Tittes, Sabine Schwarzer, Friedhelm Pfeiffer, Mike Dyall-Smith, Mike Dyall-Smith, Marta Rodriguez-Franco, Hanna M. Oksanen, Tessa E. F. Quax   +7 more
doaj   +1 more source

A Small RNA Is Linking CRISPR–Cas and Zinc Transport

Frontiers in Molecular Biosciences, 2021
The function and mode of action of small regulatory RNAs is currently still understudied in archaea. In the halophilic archaeon Haloferax volcanii, a plethora of sRNAs have been identified; however, in-depth functional analysis is missing for most of ...
Pascal Märkle, Lisa-Katharina Maier, Sandra Maaß, Claudia Hirschfeld, Jürgen Bartel, Dörte Becher, Björn Voß, Anita Marchfelder   +7 more
doaj   +1 more source

Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei [PDF]

Journal of Bacteriology, 1995
Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-point chromosomal comparison revealed
P, López-García, A, St Jean, R, Amils, R L, Charlebois   +3 more
openaire   +2 more sources