Results 11 to 20 of about 5,166 (196)

Dihydroxyacetone metabolism in Haloferax volcanii [PDF]

Frontiers in Microbiology, 2013
Dihydroxyacetone (DHA) is a ketose sugar that can be produced by oxidizing glycerol. DHA in the environment is taken up and phosphorylated to DHA-phosphate by glycerol kinase or DHA kinase.
Matthew eOuellette, Andrea M Makkay, R Thane ePapke   +2 more
doaj   +3 more sources

Extracellular DNA metabolism in Haloferax volcanii [PDF]

Frontiers in Microbiology, 2014
Extracellular DNA is found in all environments and is a dynamic component of the micro-bial ecosystem. Microbial cells produce and interact with extracellular DNA through many endogenous mechanisms.
Scott eChimileski, Kunal eDolas, Adit eNaor, Uri eGophna, R Thane ePapke   +4 more
doaj   +3 more sources

Chase Assay of Protein Stability in Haloferax volcanii

Bio-Protocol, 2017
Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study ...
Xian Fu, Julie Maupin-Furlow
doaj   +4 more sources

N-glycosylation in Haloferax volcanii: Adjusting the sweetness [PDF]

Frontiers in Microbiology, 2013
Long believed to be restricted to Eukarya, it is now known that cells of all three domains of life perform N-glycosylation, the covalent attachment of glycans to select target protein asparagine residues.
Jerry eEichler, Adi eArbiv, Chen eCohen-Rosenzweig, Lina eKaminski, Lina eKandiba, Zvia eKonrad   +5 more
doaj   +3 more sources

DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. [PDF]

iScience
Summary: This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca.
Oleg N. Reva, Violetta La Cono, Laura Marturano, Francesca Crisafi, Francesco Smedile, Manasi Mudaliyar, Debnath Ghosal, Elena A. Selivanova, Marina E. Ignatenko, Manuel Ferrer, Laura Fernandez-Lopez, Mart Krupovic, Michail M. Yakimov   +12 more
doaj   +2 more sources

Revisiting synthetic lethality of Gcn5-related N-acetyltransferase (GNAT) family mutations in Haloferax volcanii [PDF]

Microbiology Spectrum
Lysine acetylation is a post-translational modification that occurs in all domains of life, highlighting its evolutionary significance. Previous genome comparison identified three Gcn5-related N-acetyltransferase (GNAT) family members as lysine ...
Katherine R. Weber, Brianna Novillo, Julie A. Maupin-Furlow   +2 more
doaj   +2 more sources

Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring

Bio-Protocol, 2022
The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions.
Paula Mondragon, Sungmin Hwang, Amy Schmid, Julie Maupin-Furlow   +3 more
doaj   +2 more sources

A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification

Frontiers in Microbiology
Extremophilic proteins are valuable in various fields, but their expression can be challenging in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax volcanii (H.
Ram Karan, Dominik Renn, Thorsten Allers   +2 more
exaly   +3 more sources

Investigation of the global translational response to oxidative stress in the model archaeon Haloferax volcanii reveals untranslated small RNAs with ribosome occupancy [PDF]

mSphere
Oxidative stress induces a wide range of cellular damage, often causing disease and cell death. While many organisms are susceptible to the effects of oxidative stress, haloarchaea have adapted to be highly resistant.
Emma Dallon, Haley M. Moran, Sadhana R. Chidambaran, Arman Kian, Betty Y. H. Huang, Stephen D. Fried, Jocelyne DiRuggiero   +6 more
doaj   +2 more sources

Archaeal Persisters: Persister Cell Formation as a Stress Response in Haloferax volcanii

Frontiers in Microbiology, 2017
Persister cells are phenotypic variants within a microbial population, which are dormant and transiently tolerant to stress. Persistence has been studied extensively in bacteria, and in eukaryotes to a limited extent, however, it has never been observed ...
Julianne Megaw, Brendan F Gilmore
exaly   +3 more sources