Results 11 to 20 of about 5,137 (188)
The Adaptive Immune System of Haloferax volcanii [PDF]
Life, 2015 To fight off invading genetic elements, prokaryotes have developed an elaborate defence system that is both adaptable and heritable—the CRISPR-Cas system (CRISPR is short for: clustered regularly interspaced short palindromic repeats and Cas: CRISPR ...
Lisa-Katharina Maier +2 more
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Dihydroxyacetone metabolism in Haloferax volcanii [PDF]
Frontiers in Microbiology, 2013 Dihydroxyacetone (DHA) is a ketose sugar that can be produced by oxidizing glycerol. DHA in the environment is taken up and phosphorylated to DHA-phosphate by glycerol kinase or DHA kinase.
Matthew eOuellette +2 more
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Extracellular DNA metabolism in Haloferax volcanii [PDF]
Frontiers in Microbiology, 2014 Extracellular DNA is found in all environments and is a dynamic component of the micro-bial ecosystem. Microbial cells produce and interact with extracellular DNA through many endogenous mechanisms.
Scott eChimileski +4 more
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Chase Assay of Protein Stability in Haloferax volcanii
Bio-Protocol, 2017 Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study ...
Xian Fu, Julie Maupin-Furlow
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N-glycosylation in Haloferax volcanii: Adjusting the sweetness [PDF]
Frontiers in Microbiology, 2013 Long believed to be restricted to Eukarya, it is now known that cells of all three domains of life perform N-glycosylation, the covalent attachment of glycans to select target protein asparagine residues.
Jerry eEichler +5 more
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Structural and functional adaptation of Haloferax volcanii TFEα/β [PDF]
Nucleic Acids Research, 2018 The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit.
Blombach, F +9 more
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Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii [PDF]
Nature, 2010 Archaea, one of three major evolutionary lineages of life, encode proteasomes highly related to those of eukaryotes. In contrast, archaeal ubiquitin-like proteins are less conserved and not known to function in protein conjugation. This has complicated our understanding of the origins of ubiquitination and its connection to proteasomes.
Humbard, Matthew A. +8 more
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Regulated Iron Siderophore Production of the Halophilic Archaeon Haloferax volcanii [PDF]
Biomolecules, 2020 Iron is part of many redox and other enzymes and, thus, it is essential for all living beings. Many oxic environments have extremely low concentrations of free iron. Therefore, many prokaryotic species evolved siderophores, i.e., small organic molecules that complex Fe3+ with very high affinity.
Natalie Niessen, Jörg Soppa
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Archaeal Persisters: Persister Cell Formation as a Stress Response in Haloferax volcanii
Frontiers in Microbiology, 2017 Persister cells are phenotypic variants within a microbial population, which are dormant and transiently tolerant to stress. Persistence has been studied extensively in bacteria, and in eukaryotes to a limited extent, however, it has never been observed ...
Julianne Megaw, Brendan F. Gilmore
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Revisiting synthetic lethality of Gcn5-related N-acetyltransferase (GNAT) family mutations in Haloferax volcanii [PDF]
Microbiology SpectrumLysine acetylation is a post-translational modification that occurs in all domains of life, highlighting its evolutionary significance. Previous genome comparison identified three Gcn5-related N-acetyltransferase (GNAT) family members as lysine ...
Katherine R. Weber +2 more
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