Results 141 to 150 of about 1,335 (158)

Crystallographic insights into lipid-membrane protein interactions in microbial rhodopsins. [PDF]

open access: yesFront Mol Biosci
Bukhdruker S   +4 more
europepmc   +1 more source
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Dihydroxyacetone metabolism in Salinibacter ruber and in Haloquadratum walsbyi

Extremophiles, 2007
The extremely halophilic bacterium Salinibacter ruber inhabits saltern crystallizer ponds worldwide, together with the square archaeon Haloquadratum walsbyi. Cultures of Salinibacter have been shown to convert up to 20% of the glycerol added to a not previously characterized overflow product.
Aharon Oren
exaly   +3 more sources

Haloquadratum walsbyi Yields a Versatile, NAD+/NADP+ Dual Affinity, Thermostable, Alcohol Dehydrogenase (HwADH) [PDF]

open access: yesMolecular Biotechnology, 2018
This study presents the first example of an alcohol dehydrogenase (ADH) from the halophilic archaeum Haloquadratum walsbyi (HwADH). A hexahistidine-tagged recombinant HwADH was heterologously overexpressed in Haloferax volcanii. HwADH was purified in one step and was found to be thermophilic with optimal activity at 65 °C.
Francesca Paradisi
exaly   +3 more sources

Diversity of Haloquadratum and other haloarchaea in three, geographically distant, Australian saltern crystallizer ponds [PDF]

open access: yesExtremophiles, 2009
Haloquadratum walsbyi is frequently a dominant member of the microbial communities in hypersaline waters. 16S rRNA gene sequences indicate that divergence within this species is very low but relatively few sites have been examined, particularly in the southern hemisphere.
Kate Porter, Michael Dyall-Smith
exaly   +5 more sources

Structural and Functional Studies of a Newly Grouped Haloquadratum walsbyi Bacteriorhodopsin Reveal the Acid-resistant Light-driven Proton Pumping Activity [PDF]

open access: yesJournal of Biological Chemistry, 2015
Retinal bound light-driven proton pumps are widespread in eukaryotic and prokaryotic organisms. Among these pumps, bacteriorhodopsin (BR) proteins cooperate with ATP synthase to convert captured solar energy into a biologically consumable form, ATP. In an acidic environment or when pumped-out protons accumulate in the extracellular region, the maximum ...
Hsu-Yuan Fu   +2 more
exaly   +3 more sources

Protein splicing and tryptophan fluorescence of salt‐dependent inteins from Haloquadratum walsbyi

The FASEB Journal, 2020
Protein splicing is a self‐catalyzed reaction by which an intein, an intervening polypeptide, catalyzes its own removal from exteins, the flanking polypeptides, as well as ligation of the exteins. We are studying the inteins that interrupt a cell division control protein in the halophilic archaea Haloquadratum ...
Vaishnavi Yalala   +3 more
openaire   +1 more source

A Directed Evolution Experiment to Improve Protein Splicing of Two Inteins From Haloquadratum walsbyi

FASEB Journal, 2022
We are interested in the protein splicing of the inteins from the extreme halophile Haloquadratum walsbyi. Protein splicing is a post translational process by which an intein excises itself and ligates the two flanking polypeptides, the exteins. Two inteins interrupt the cell division control (cdc21) protein in H. walsbyi.
Anthony C. D'Angelo   +2 more
exaly   +2 more sources

Differential Protein Splicing of Salt Dependent Inteins from Haloquadratum Walsybi

The FASEB Journal, 2017
Protein splicing is a self‐catalyzed, post‐translational process in which an intervening polypeptide (intein) catalyzes its own removal from the flanking polypeptides (exteins), as well as the ligation of the exteins.
Abigeal Kathleen Lynch   +3 more
openaire   +1 more source

The Light‐Activated Proton Pump Bop I of The Archaeon Haloquadratum walsbyi

Photochemistry and Photobiology, 2012
AbstractWe have isolated and characterized the light‐driven proton pump Bop I from the ultrathin square archaeon Haloquadratum walsbyi, the most abundant component of the dense microbial community inhabiting hypersaline environments. The disruption of cells by hypo‐osmotic shock yielded Bop I retinal protein highly enriched membranes, which contain one
Simona Lobasso   +8 more
openaire   +3 more sources

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