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Dunaliella salina Hsp90 is halotolerant

International Journal of Biological Macromolecules, 2015
Dunaliella salina is a unicellular green alga with exceptional halotolerance. Although the D. salina cells are capable to proliferate in hypersaline medium, the intracellular salt concentrations are maintained at a low level. Thus the extracellular but not intracellular Dunaliella proteins are expected to be highly halotolerant.
Xiang-Jun, Chen   +4 more
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Molecular bases of protein halotolerance

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2014
Halophilic proteins are stable and function at high salt concentration. Understanding how these molecules maintain their fold stable and avoid aggregation under harsh conditions is of great interest for biotechnological applications. This mini-review describes what is known about the molecular determinants of protein halotolerance.
Giuseppe Graziano, MERLINO, ANTONELLO
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Halotolerance mechanisms in salt‑tolerant cyanobacteria

2023
Cyanobacteria are ubiquitously distributed in nature and are the most abundant photoautotrophs on Earth. Their long evolutionary history reveals that cyanobacteria have a remarkable capacity and strong adaptive tendencies to thrive in a variety of conditions.
Hakuto, Kageyama   +1 more
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Hydroxylamine reductase of a halotolerant micrococcus

Biochimica et Biophysica Acta, 1960
A hydroxylamine reductase of hemoprotein nature was prepared from a strain of halotolerant Micrococcus. The hydroxylamine reductase was shown to contain iron as its active center. Manganese ions at 0.5 mM concentration activate the reaction about 3 to 4 times when the activity is measured by the reduced methylene blue method; these ions possibly ...
M, KONO, S, TANIGUCHI
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Mechanisms of Halotolerance in Microorganisms

CRC Critical Reviews in Microbiology, 1987
Microorganisms have the ability to adapt to a wide range of NaCl concentrations. In general the NaCl tolerance shown by microbes far exceeds the salt tolerance of any other organism, procryote or eukaryote. There are at least three mechanisms available for adaptation to different salt concentrations.
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Kinetic and thermodynamic characterization of a halotolerant β‐galactosidase produced by halotolerant Aspergillus tubingensis GR1

Journal of Basic Microbiology, 2015
β‐Galactosidase from halotolerant Aspergillus tubingensis GR1 was purified by two‐step purification process comprising ammonium sulfate precipitation followed by size exclusion chromatography (SEC). The recovery of β‐galactosidase after SEC was found to be 1.40% with 58.55‐fold increase in specific activity.
Gopalkumar G, Raol   +3 more
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Halophily and halotolerance in cyanophytes

Origins of Life, 1980
The survival, growth and distribution of organisms in hypersaline environments is discussed using cyanophytes (cyanobacteria) as examples. The distinction between halophilic (Na+-requiring) and halotolerant organisms is not adequate to describe the entire spectrum of adaptations to salt.
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Na+-ATPases of halotolerant microalgae

Russian Journal of Plant Physiology, 2013
Na+ homeostasis in the cytoplasm is a common property of all organisms, irrespective of their taxonomic position. Low Na+ concentrations in the cytoplasm of living cells are maintained by specialized Na+-transporting molecular machines operating in the cell membranes.
L. G. Popova, Yu. V. Balnokin
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Characterization of a metagenome-derived halotolerant cellulase

Journal of Biotechnology, 2006
Metagenomes of uncultured microorganisms represent a sheer unlimited resource for discovery of novel biocatalysts. Here, we report on the biochemical characterisation of a novel, soil metagenome-derived cellulase (endoglucanase), Cel5A. The deduced amino acid sequence of Cel5A was similar to a family 5, single domain cellulase with no distinct ...
S, Voget, H L, Steele, W R, Streit
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Glycolipids of a halotolerant, moderately halophilic bacterium

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1978
A 247 000 x g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [14C] glucose added as UDP[14C]glucose and 32P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication ...
N, Stern, A, Tietz
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