Results 131 to 140 of about 36,717 (236)
Reductive Methylation: An Alternative to Lysine → Arginine Mutagenesis
Replacing lysine with arginine, the standard genetic approach to eliminate amine reactivity, alters physicochemical properties and leaves the N terminus unprotected. Here, reductive methylation is characterized as a conservative chemical alternative. Complete dimethylation of human ribonucleases preserves thermostability, protein–protein interaction ...
Oscar J. Molina +4 more
wiley +1 more source
Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice
ABSTRACT Background Sperm motility is essential for male fertility, and its regulation is dependent on the structural integrity of the axoneme. The axoneme consists of a conserved “9+2” microtubule arrangement and is supported by microtubule inner proteins. However, the functional significance of many microtubule inner proteins remains unclear.
Haoting Wang +4 more
wiley +1 more source
The development of homozygous breeding lines in maize may be accelerated through the use of haploids. Thus, the obtaining and prior identification of haploids generated by the haploid inducer lines is an important factor. The purpose of this study was to
Evellyn Giselly de Oliveira Couto +4 more
doaj
We identified a Mis4 cohesin loader mutant that is specifically defective in chromatin axis formation and homologous recombination while retaining normal sister chromatid cohesion. The mis4‐LR mutant, carrying the L1150S and R1159G amino acid substitutions, exhibited reduced interaction between Mis4 and Rec8, suggesting a meiosis‐specific role for the ...
Takeshi Sakuno +3 more
wiley +1 more source
Double-fluorescent proteins enable robust maternal haploid identification in wheat. [PDF]
Li Y +15 more
europepmc +1 more source
Progress and Challenges in Phylogenomics and Genomics of Lophotrochozoa/Spiralia
ABSTRACT Lophotrochozoa is one of the three major bilaterian groups comprising more than half of the bilaterian phyla. Lophotrochozoa includes among others Mollusca, Annelida, Platyhelminthes and Rotifera. Despite representing such a large proportion of animal diversity, they are historically understudied and genomic resources have been scarce. However,
Torsten H. Struck
wiley +1 more source
Karyotype analysis in haploid sugarbeet
5 Pags.- 1 Tabl.- 4 Figs. Publisher's version downloading from JSTOR (Stable URL: http://www.jstor.org/stable/2474750 ; Publisher's version/PDF may be used in open access repositories only)The objective of this study was to develop a standard metaphase
Lasa Dolhagaray, José Manuel +3 more
core
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
Unifying Retaria Research: A Common Framework for Studying Foraminifera and Radiolaria
Research on Foraminifera and Radiolaria has predominantly focused on their fossil remains. This synthesis identifies the “Big Five” disciplines of a unified Retaria research: diversity and evolution, ecology, life cycles, and ecosystem roles, highlighting this lineage as a model system for understanding life's resilience in a rapidly changing planet ...
Miguel M. Sandin +6 more
wiley +1 more source
Zebrafish illustrates how polygenic sex determination, maternal germline bottlenecks, and a structurally restless genome jointly destabilize deep homozygosity. These interlocking constraints make fully isogenic zebrafish lines effectively unattainable, turning this model into a natural “stress test” for assumptions about genetic background and ...
Álvaro J. Arana, Laura Sánchez
wiley +1 more source

