Results 91 to 100 of about 86,009 (325)
Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination
BMC Biotechnology, 2020 Background Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR).
Sanum Bashir +4 more
doaj +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Debaryomyces hansenii is a yeast with diverse potential applications like producing oleochemicals from biomass. Yet, combinatorial metabolic engineering in D. hansenii is limited because, like many nonconventional organisms, key genomic data and genetic elements are missing. Here, we report phenotypic characterization, genomic integration loci,
Zekun Li +3 more
wiley +1 more source
Fhod3 in zebrafish supports myofibril stability during growth of embryonic skeletal muscle
Developmental Dynamics, EarlyView.Abstract Background Actin filament organization in cardiomyocytes critically depends on the formin Fhod3, but a role for Fhod3 in skeletal muscle development has not yet been described. Results We demonstrate here that in zebrafish mutated for one of two fhod3 paralog genes, fhod3a, skeletal muscle of the trunk appears normal through 2 days post ...
Aubrie Russell +3 more
wiley +1 more source
Methodologies for Improving HDR Efficiency
Frontiers in Genetics, 2019 Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) is a precise genome manipulating technology that can be programmed to induce double-strand break (DSB) in the genome wherever needed.
Ming-Jie Liu +6 more
semanticscholar +1 more source
Green paper Consultation on recommendations for developing Population Research UK
, 2021 Population Research UK is an initiative by ESRC, MRC and Wellcome, being scoped on their behalf by HDR UK. After an initial consultation earlier this year on the needs and opportunities of UK longitudinal population studies, HDR UK has developed a set of
HDR UK
core +1 more source
Earthquake Engineering &Structural Dynamics, EarlyView.ABSTRACT Conventional unbonded elastomeric bearings (ULNRs) are cost‐effective but prone to sliding during earthquakes due to their limited shear deformability. Unbonded Steel‐Mesh‐Reinforced Rubber Bearings (USRBs) offer a solution with increased deformation capacity.
Han Li, Jie Shen, M. Shahria Alam
wiley +1 more source
Journal of Intelligent SystemsHandwritten digit recognition (HDR) remains challenging due to variations in writing styles. To address this challenge, this study comprehensively compares ML (ML) and deep learning (DL) models. We explored a variety of approaches.
Ben Noureddine Dhouha
doaj +1 more source
Flavour and Fragrance Journal, EarlyView.This review bridges ancestral indigenous knowledge with modern pharmacology, summarizing the phytochemistry and antimicrobial properties of essential oils from seven native American species. It highlights their significant activity against bacterial, fungal and parasitic pathogens, emphasizing their potential as sustainable therapeutic and agricultural
Arley Rey Páez +2 more
wiley +1 more source
Lien Social et Politiques, 2017 Les courses alimentaires occupent une place centrale dans la vie quotidienne des personnes âgées, cette activité constituant le premier motif de sortie de celles vivant en centre-ville.
Philippe Cardon
doaj +1 more source