Results 91 to 100 of about 2,796,382 (285)
Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription
FEBS Open Bio, EarlyView.In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang +10 more
wiley +1 more source
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Non-monotonic energy flux density profiles at the ITER divertor entrance
Nuclear FusionSOLPS-ITER simulations of the ITER baseline ( Q = 10) plasma boundary, with tungsten (W) targets and drift terms activated, exhibit a radially localised peak in the energy flux density entering the outer divertor, despite constant anomalous transport ...
D. Moulton, R.A. Pitts, X. Bonnin
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Heat stress in dairy cows: A review of abiotic and biotic factors, with reference to the subtropics
South African Journal of Animal ScienceHeat stress has been identified as one of the major challenges for livestock production. Global temperatures are steadily increasing, with South African temperatures increasing at nearly twice the global rate.
LM Erasmus, E van Marle-Köster
doaj +1 more source
Stability of Bragg reflectors under megahertz heat load at XFELs. [PDF]
J Synchrotron Radiat, 2023 Bahns I, Rauer P, Rossbach J, Sinn H.
europepmc +1 more source
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Automated Quantification of the Behaviour of Beef Cattle Exposed to Heat Load Conditions. [PDF]
Animals (Basel), 2023 Idris M +5 more
europepmc +1 more source