Results 71 to 80 of about 176,436 (256)

Estudio exergético del espacio climatizado en una embarcación fluvial;Exergy Study of air-conditioned space on a river vessel.

Revista Científica de Ingeniería Energética, 2015
En este trabajo se presentan los resultados del análisis exergético realizado al sistema de acondicionamiento de aire de una embarcación fluvial. El estudio se realizó en un prototipo a escala de laboratorio, el cual simula uno de los locales de la ...
Juan Gabriel Fajardo Cuadro, Bienvenido Sarria López, Mario Álvarez Guerra Plasencia   +2 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

On strongly loaded heat equations

BULLETIN OF THE KARAGANDA UNIVERSITY-MATHEMATICS, 2019
The article is devoted to the research of boundary value problems for the spectrum - loaded operator of heat conduction with the moving point of loading to the temporary axle in zero or on infinity. For strongly loaded parabolic 2k - order equations the adjoint boundary value problems, when order of loaded term is greater then one of differential part ...
D.M. Akhmanova, M.T. Kosmakova, B.A. Shaldykova   +2 more
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Non-monotonic energy flux density profiles at the ITER divertor entrance

Nuclear Fusion
SOLPS-ITER simulations of the ITER baseline ( Q  = 10) plasma boundary, with tungsten (W) targets and drift terms activated, exhibit a radially localised peak in the energy flux density entering the outer divertor, despite constant anomalous transport ...
D. Moulton, R.A. Pitts, X. Bonnin
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Heat stress in dairy cows: A review of abiotic and biotic factors, with reference to the subtropics

South African Journal of Animal Science
Heat stress has been identified as one of the major challenges for livestock production. Global temperatures are steadily increasing, with South African temperatures increasing at nearly twice the global rate.
LM Erasmus, E van Marle-Köster
doaj   +1 more source

Stability of Bragg reflectors under megahertz heat load at XFELs. [PDF]

J Synchrotron Radiat, 2023
Bahns I, Rauer P, Rossbach J, Sinn H.
europepmc   +1 more source