Results 131 to 140 of about 764,920 (304)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Study on high temperature combustion characteristic parameters of coking coal with different particle sizes

Meikuang Anquan
In order to study the combustion characteristics of coal samples with different particle sizes under high temperature conditions, the combustion characteristic parameters of coking coal and its mixed coal samples with particle sizes of 0.200-0.450 mm, 0 ...
Xiumin XIE, Leilin ZHANG
doaj   +1 more source

Characterization and modeling of aperiodic pressure oscillations in combustion chambers [PDF]

, 1991
Classification of the long-term dynamical behavior of pressure oscillations in a laboratory combustion chamber has been performed using methods of modern dynamical systems theory. The method involves the construction of a phase-space representation
Sterling, James D.
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Burning Properties of Combined Glued Laminated Timber

Fire
This study delved into the combustion properties of combined glulam bonded using polyurethane (PUR) and resorcinol-phenol-formaldehyde (RPF) adhesives.
Tomáš Kytka, Miroslav Gašparík, David Novák, Lukáš Sahula, Elham Karami, Sumanta Das   +5 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Heat Storage and Release Characteristics of Nanosuspension Storage Material

Netsu Bussei, 2017
Shin-ichi Morita, Koki Hirata, Yasutaka Hayamizu, Takanobu Yamada, Akihiko Horibe, Naoto Haruki   +5 more
openaire   +2 more sources

The Lunar Environment [PDF]

, 1960
The NASA lunar exploration program has focused the attention of many scientific and technical groups on such questions as the relative value of lunar experiments, the advantages of manned versus unmanned exploration, and the proper time sequence for the ...
Buwalda, Phyllis
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source