Results 31 to 40 of about 93,819 (310)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Peristaltic motion of Maxwell fluid subject to convective heat and mass conditions

Ain Shams Engineering Journal, 2021
This article concentrates on peristalsis of Maxwell fluid in a symmetric channel with heat and mass convective conditions. Analysis is given in presence of viscous dissipation.
Naveed Iqbal, Humaira Yasmin, Aneela Bibi, Adel A. Attiya   +3 more
doaj   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Enhancing Corrosion Resistance and Mechanical Strength of 3D‐Printed Iron Polylactic Acid for Marine Applications via Laser Surface Texturing

Advanced Engineering Materials, EarlyView.
Laser surface texturing significantly improves the corrosion resistance and mechanical strength of 3D‐printed iron polylactic acid (Ir‐PLA) for marine applications. Optimal laser parameters reduce corrosion by 80% and enhance tensile strength by 25% and ductility by 15%.
Mohammad Rezayat, Mojtaba Karamimoghadam, Mohammadreza Lalegani Dezaki, Ali Zolfagharian, Giuseppe Casalino, Antonio Mateo, Mahdi Bodaghi   +6 more
wiley   +1 more source

Study on comprehensive performance evaluation for condensation heat transfer inside the micro-fin tube

Advances in Mechanical Engineering, 2020
To develop a comprehensive performance evaluation model of heat transfer tube, the flow condensation heat transfer experiment of R1234yf inside the tube was operated.
Liu Wei
doaj   +1 more source

Characterization of Defect Distribution in an Additively Manufactured AlSi10Mg as a Function of Processing Parameters and Correlations with Extreme Value Statistics

Advanced Engineering Materials, EarlyView.
Predicting extreme defects in additive manufacturing remains a key challenge limiting its structural reliability. This study proposes a statistical framework that integrates Extreme Value Theory with advanced process indicators to explore defect–process relationships and improve the estimation of critical defect sizes. The approach provides a basis for
Muhammad Muteeb Butt, Varma Kvvssn, Hossein Laieghi, Zia Uddin, Peyman Ansari, E. Kıvanç Sadak, Can Barış Toprak, Hüseyin Kızıl, Enrico Salvati   +8 more
wiley   +1 more source

Laser Metal Deposited Ti4822 Hollow Pipe: Experimental and Computational Modelling Study

Advanced Engineering Materials, EarlyView.
Laser metal deposition (LMD) of a crack‐free built Ti4822 alloys is challenging. This article reports outstanding characteristics of a hollow pipe that is built with LMD technology when a predicted, nontransformation substrate temperature of 800 °C is used.
Sadiq A. Raji, Monnamme Tlotleng, Samuel Skhosane, Lehlogonolo R. Kanyane, Abimbola P. I. Popoola, Sisa L. Pityana   +5 more
wiley   +1 more source

Numerical Model and Experimental Validation of Composite Metal Foam in Protecting Carbon Steel Against Puncture

Advanced Engineering Materials, EarlyView.
The puncture prevention and energy absorption performance of composite metal foam (CMF) are studied experimentally and numerically for hazardous materials transportation protection. The numerical model implementing air within the CMF (nonhomogeneous model using fluid cavity technique) predicts puncture more accurately compared to the homogeneous CMF ...
Aman Kaushik, Afsaneh Rabiei
wiley   +1 more source

Study on Natural Circulation Heat Transfer Characteristics of Different Liquid Metals Based on Factor Analysis

Frontiers in Energy Research, 2020
The study of the commonness and differences of natural circulation of different liquid metals plays an important role in improving the safety of reactors.
Feng Xiang, Feng Xiang, Feng Xiang, Zhou Tao, Zhou Tao, Zhou Tao, Zhang Jialei, Zhang Jialei, Zhang Jialei, Zhang Boya, Zhang Boya, Zhang Boya, Chen Juan, Chen Juan, Chen Juan   +14 more
doaj   +1 more source