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Proximity biotinylation at the host-Shigellainterface reveals UFMylation as an antibacterial pathway
López-Jiménez AT +15 more
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Methods in molecular biology, 2011
HeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studying the cell cycle. Here, we describe several protocols for synchronizing HeLa cells from different phases of the cell cycle, including G1 ...
Ma, Hoi Tang, Poon, Randy Y.C.
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HeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studying the cell cycle. Here, we describe several protocols for synchronizing HeLa cells from different phases of the cell cycle, including G1 ...
Ma, Hoi Tang, Poon, Randy Y.C.
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Science, 1976 A list is presented of references to all known publications on properties which have served to relate strains of HeLa cells to each other as well as to indict other purported human cell lines as HeLa cell contaminants. Eleven additional cell lines not previously indicted are described.
Walter A. Nelson-Rees +1 more
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Binding of hela spectrin to a specific hela membrane fraction
Cell Motility, 1983AbstractFrom 30–40 g of Hela‐S3 cells grown in suspension, 0.25–0.50 mg of spectrin has been purified by conventional biochemical procedures starting from a low ionic strength extraction at alkaline pH of crude Hela membranes. Hela spectrin consists in its native form of a tetramer α2β2 of two high molecular weight polypeptides (240,000 and 230,000 ...
Keith Burridge, Paul Mangeat
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2022
HeLa cells were embedded in Durcupan and observed with a Serial Block Face Scanning Electron Microscope (SBF SEM) following the National Centre for Microscopy and Imaging Research (NCMIR) protocol. The images were acquired with an SBF SEM 3View2XP microscope (Gatan, Pleasanton, CA, USA) attached to a Sigma VP SEM (Zeiss, Cambridge, UK).
Karabag, Cefa +1 more
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HeLa cells were embedded in Durcupan and observed with a Serial Block Face Scanning Electron Microscope (SBF SEM) following the National Centre for Microscopy and Imaging Research (NCMIR) protocol. The images were acquired with an SBF SEM 3View2XP microscope (Gatan, Pleasanton, CA, USA) attached to a Sigma VP SEM (Zeiss, Cambridge, UK).
Karabag, Cefa +1 more
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Differential calcium response in HeLa and HeLa-Fas cells by cytotoxic T lymphocytes
Biochemical and Biophysical Research Communications, 2003We constructed a CD95 overexpressing HeLa cell line which was extremely sensitive towards CD95 mediated apoptosis. In these CD95 overexpressing cells, CD95 blocks the nuclear calcium signal induced by perforin positive and CD95 ligand positive killer cells.
E. Marion Schneider +3 more
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Preparation of multicolor emitting carbon dots for HeLa cell imaging
, 2014We have synthesized biocompatible fluorescent carbon dots (CDs) by a one-step hydrothermal method using Solanum tuberosum (potato) as a raw material.
Vaibhavkumar N. Mehta +3 more
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Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes
Cytogenetic and Genome Research, 1988In contrast to earlier reports, this study on HeLa, HeLa S3, and HEp-2 revealed that karyotypes of each cell line are characterized by a consistent marker chromosome composition and a constant number of copies for both normal and marker chromosomes. Based on these chromosome fingerprints and an analysis of 50 metaphases, the modal karyotype of each ...
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ACS Nano, 2009
Upconversion fluorescent nanoparticles can convert a longer wavelength radiation (e.g., near-infrared light) into a shorter wavelength fluorescence (e.g., visible light) and thus have emerged as a new class of fluorescent probes for biomedical imaging ...
Meng Wang +7 more
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Upconversion fluorescent nanoparticles can convert a longer wavelength radiation (e.g., near-infrared light) into a shorter wavelength fluorescence (e.g., visible light) and thus have emerged as a new class of fluorescent probes for biomedical imaging ...
Meng Wang +7 more
semanticscholar +1 more source
Parasynchronous division of HeLa cells
Experimental Cell Research, 1959Abstract It has been observed that populations of human cervical carcinoma cells (strain HeLa) may be induced to undergo parasynchronous division by chilling of the cell culture for a short period. Replicate cultures of HeLa cells were incubated at 37 °C for 24 hours after subculture and then placed at 4 °C for 1 hour.
P. Wildy, A.A. Newton
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