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Photoactivatable Alkyne Tag for Photolabeling Biomolecules in Living Cells

open access: yesChemBioChem, EarlyView.
A photoactivatable linear alkyne tag is developed by caging the intermediate of the Eschenmoser–Tanabe reaction to expand photoinduced intracellular molecular imaging to a variety of biomolecules. The photoactivatable tag is modified onto a cholesterol analog and introduced into cells. After light irradiation, the tag is successfully labeled with azide‐
Yuki Umeda   +6 more
wiley   +1 more source

Recombinant Production of TP4-LYC1, A New Chimeric Peptide with Targeted Cytotoxicity to HeLa Cells. [PDF]

open access: yesAvicenna J Med Biotechnol
Mohammad Pour H   +2 more
europepmc   +1 more source

Mammalian Tolerance to Amino Acid Heterochirality

open access: yesChemBioChem, Accepted Article.
Organisms use amino acids predominantly in l‐configuration. On the other hand, a series of studies show that a variety of d‐amino acids also occur in mammals and amino acid homochirality is not complete. Mammals de novo synthesize most amino acids with l‐configuration, but serine and aspartate are converted from l‐ to d‐configuration by endogenous ...
Jumpei Sasabe   +4 more
wiley   +1 more source
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Segmentation of HeLa cells

2022
HeLa cells were embedded in Durcupan and observed with a Serial Block Face Scanning Electron Microscope (SBF SEM) following the National Centre for Microscopy and Imaging Research (NCMIR) protocol. The images were acquired with an SBF SEM 3View2XP microscope (Gatan, Pleasanton, CA, USA) attached to a Sigma VP SEM (Zeiss, Cambridge, UK).
Karabag, Cefa   +1 more
openaire   +1 more source

Synchronization of HeLa Cells

2011
HeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studying the cell cycle. Here, we describe several protocols for synchronizing HeLa cells from different phases of the cell cycle, including G1 ...
Ma, Hoi Tang, Poon, Randy Y.C.
openaire   +6 more sources

Parasynchronous division of HeLa cells

Experimental Cell Research, 1959
Abstract It has been observed that populations of human cervical carcinoma cells (strain HeLa) may be induced to undergo parasynchronous division by chilling of the cell culture for a short period. Replicate cultures of HeLa cells were incubated at 37 °C for 24 hours after subculture and then placed at 4 °C for 1 hour.
P. Wildy, A.A. Newton
openaire   +3 more sources

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