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Investigating heterogeneity in HeLa cells [PDF]
Multi-omic study uncovers biological variation across 14 HeLa cell samples, which might help to explain the growing concerns about reproducibility issues in cell culture.
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The biotin requirement of HeLa cells
Journal of Cellular Physiology, 1981AbstractWe have examined the effect of alterations in the biotin content of the medium on the growth, viability, biotin content, and the activities of biotin‐dependent and biotin‐independent enzymes of the HeLa cells. The inclusion in the growth medium of avidin, which almost irreversibly binds with biotin (Kd, 10−15 M), results in an increase in ...
K. Dakshinamurti, L. E. Chalifour
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Nature, 2011
To find out what distinguishes one cell type from another, cell biologists must renounce popular cell lines, argue Anthony H. Hyman and Kai Simons.
Anthony H. Hyman, Kai Simons
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To find out what distinguishes one cell type from another, cell biologists must renounce popular cell lines, argue Anthony H. Hyman and Kai Simons.
Anthony H. Hyman, Kai Simons
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Ribosome Assembly in Hela Cells
Nature, 1972The 80S nucleolar particles have been found to contain peptides characteristic of 30S as well as 50S ribosomal subunits. This lends support to the idea that the maturation of both types of subunits stems from this 80S particle.
B. E. H. Maden, J. Shepherd
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MITOCHONDRIAL PROTEIN SYNTHESIS IN HeLa CELLS [PDF]
ABSTRACT HeLa cell mitochondrial polysomes, estimated to consist of two to seven 60S monomers, exhibit an unusual resistance to RNase and EDTA, possibly related to the strongly hydrophobic nature of their polypeptide products which makes the nascent chains particularly "sticky".
G. Attardi+5 more
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1988
The cytotoxic action of chemicals which transfer electrons from biological reductants to molecular oxygen is believed to result from the formation of •O2-, H2O2, and •OH. Paraquat is one such agent which appears to act in Escherichia coli primarily by generating •O2-, but necessarily H2O2 as well.
Judith Krall+4 more
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The cytotoxic action of chemicals which transfer electrons from biological reductants to molecular oxygen is believed to result from the formation of •O2-, H2O2, and •OH. Paraquat is one such agent which appears to act in Escherichia coli primarily by generating •O2-, but necessarily H2O2 as well.
Judith Krall+4 more
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The ultrastructure of synchronized hela cells
Journal of Cell Science, 1971ABSTRACT Synchronous populations of mitotic HeLa cells were obtained by the controlled agitation method, and a detailed morphological study of the cells in all phases of the cell cycle was under-taken to correlate variations in cell structures to known coexisting biochemical events. Auto-radiographic techniques using tritiated thymidine (
R. A. Erlandson, E. de Harven
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Subribosomal particles of HeLa cells
Experimental Cell Research, 1974Abstract Well defined ring-shaped and rectangular particles are described as intracellular components of post-ribosomal cytosol of HeLa cells. Their morphology might be identical with particles recently isolated from plasma cell tumors and independently from the culture medium of malignant cells.
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Multiplication of Solitary HeLa Cells
Nature, 1958THERE are many occasions when it is desirable to grow animal cells from solitary inocula (for example, in order to obtain clones); for this reason, it seems worthwhile to record the following conditions which allow multiplication of isolated HeLa cells.
Peter Wildy, Michael Stoker
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
Abstract 1. 1. A simple procedure to obtain DNA-free RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) from HeLa cells was developed from the preparation of extracts in a hypotonic medium in a French pressure cell. 2. 2.
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Abstract 1. 1. A simple procedure to obtain DNA-free RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) from HeLa cells was developed from the preparation of extracts in a hypotonic medium in a French pressure cell. 2. 2.
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