Results 311 to 320 of about 391,392 (354)
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Archiv f�r die gesamte Virusforschung, 1965
A strain of varicella virus was isolated and easily maintained in HeLa cells. The infection could be passed with live cells only, practically all infectivity being destroyed by disruption of the cells with sonication or repeated freeze-thawing, even if precautions were taken to favour the infectivity of nucleic acids.
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A strain of varicella virus was isolated and easily maintained in HeLa cells. The infection could be passed with live cells only, practically all infectivity being destroyed by disruption of the cells with sonication or repeated freeze-thawing, even if precautions were taken to favour the infectivity of nucleic acids.
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Investigating heterogeneity in HeLa cells
Nature Methods, 2019Multi-omic study uncovers biological variation across 14 HeLa cell samples, which might help to explain the growing concerns about reproducibility issues in cell culture.
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DNA replication sites in HeLa cells
Experimental Cell Research, 1973Abstract We previously reported experiments which led us to conclude that DNA synthesis in HeLa cells occurs in association with the nuclear membrane. Subsequent experiments which are reported here provide evidence that DNA synthesis occurs both in proximity to and at sites removed from the nuclear membrane.
R L, O'Brien, A B, Sanyal, R H, Stanton
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Chromosomal proteins of interphase HeLa cells
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1970Abstract 1. 1. We describe the properties of the proteins of isolated interphase chromosomes of HeLa cells. Histones of interphase chromosomes were fractionated by chromatography on Amberlite CG-50 and further characterized by analytical disc electrophoresis and amino acid analysis of each chromatographic fraction. 2. 2.
A, Sadgopal, J, Bonner
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Induced thermal resistance in HeLa cells
Nature, 1975IN mammalian cells, hyperthermia causes temperature-dependent changes in cell growth parameters1, reduces DNA and protein synthesis rates2 and cell metabolism3, and leads to a loss of proliferative capacity4,5. Harris6 has shown that temperature-resistant pig kidney cells can be obtained after multiple exposures to very high thermal doses (colony ...
E W, Gerner, M J, Schneider
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Deoxyuridine triphosphate nucleotidohydrolase of HeLa cells
International Journal of Biochemistry, 1980Abstract 1. 1. A kinetically pure preparation of dUTPase was prepared from HeLa cells. 2. 2. dUTPase is a 62,500 dalton pyrophosphatase. The Km of dUTPase for dUTP is 1.22 × 10−5 M± 0.19. In the presence of Mg2+ the Km is 0.47 × 10−5M. 3. 3. dUTPase is highly specific for dUTP and will not hydrolyze any naturally occurring deoxynucleoside
S, Mahagaokar, P N, Rao, A, Orengo
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The biotin requirement of HeLa cells
Journal of Cellular Physiology, 1981AbstractWe have examined the effect of alterations in the biotin content of the medium on the growth, viability, biotin content, and the activities of biotin‐dependent and biotin‐independent enzymes of the HeLa cells. The inclusion in the growth medium of avidin, which almost irreversibly binds with biotin (Kd, 10−15 M), results in an increase in ...
K, Dakshinamurti, L E, Chalifour
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Mitochondrial protein synthesis in HeLa cells
Journal of Molecular Biology, 1971Abstract A fraction of HeLa cell mitochondrial protein whose synthesis is unaffected by cycloheximide but blocked by chloramphenicol has been observed by acrylamide gel electrophoresis of protein released from mitochondrial preparations. In addition, it has been demonstrated that HeLa cells in the presence of puromycin produce formylmethionyl ...
J B, Galper, J E, Darnell
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1973
A primary role for the nucleolus in ribosome biogenesis has been well established and documented (1,2). It has been shown that the nucleolus, a well-characterized but variable nuclear morphological entity, represents the chromosomal location for the genes coding for ribosomal precursor RNA. In addition, the derivatives of this product, mature ribosomal
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A primary role for the nucleolus in ribosome biogenesis has been well established and documented (1,2). It has been shown that the nucleolus, a well-characterized but variable nuclear morphological entity, represents the chromosomal location for the genes coding for ribosomal precursor RNA. In addition, the derivatives of this product, mature ribosomal
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A cytoplasmic exoribonuclease from HeLa cells
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977An exoribonuclease has been purified from the cytoplasm of HeLa cells. The enzyme produces 5'-AMP as the only product from poly(A). The degradation proceeds in a 3' to 5' direction, and a 3'-OH terminus is required. In addition to poly(A), the enzyme degrades other synthetic homopolymers as well as natural messenger, and ribosomal RNAs.
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