Results 181 to 190 of about 2,889 (214)
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Immunostaining Helobdella (Leech) Embryos
Cold Spring Harbor Protocols, 2009INTRODUCTIONThe purpose of this staining protocol is to detect the localization of specific antigens in the Helobdella (leech) embryo. Immunostaining protocols must be optimized for each antibody and embryonic stage. Here, as a starting point, we present a general-purpose immunostaining protocol.
David A, Weisblat, Dian-Han, Kuo
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Handling of Helobdella (Leech) Embryos
Cold Spring Harbor Protocols, 2009INTRODUCTIONGravid Helobdella (leech) adults are identified by white egg masses that are visible through the ventral body wall. For convenience, gravid animals should be moved to smaller bowls so that those animals with newly laid embryos can be more readily identified.
David A, Weisblat, Dian-Han, Kuo
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Published as part of Khayrulla Solijonov, Serge Utevsky, Zuvaid Izzatullaev & Utevsky, Andriy, 2026, At the boundary of two biogeographical realms: Palearctic and Oriental leeches (Hirudinea, Glossiphoniidae) of the Ferghana Valley, Uzbekistan, pp. 41-54 in Zoosystema 48 (2) on pages 46-48, DOI: 10.5252/zoosystema2025v48a2, http://zenodo.org/record ...
Khayrulla Solijonov +3 more
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Khayrulla Solijonov +3 more
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Devitellinization of Living Helobdella (Leech) Embryos
Cold Spring Harbor Protocols, 2009INTRODUCTIONEmbryos of glossiphoniid leeches are enclosed in a thin vitelline envelope until “hatching” (stage 10). This protocol describes the procedure for removing the vitelline envelope from Helobdella robusta and H. sp. (Austin) embryos. This protocol is applicable to embryos of stages 1-9 but is probably more useful for early stages. With careful
David A, Weisblat, Dian-Han, Kuo
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In Situ Hybridization of Helobdella (Leech) Embryos
Cold Spring Harbor Protocols, 2009INTRODUCTIONThe purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus. The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference
David A, Weisblat, Dian-Han, Kuo
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Cell death in late embryogenesis of the leech Helobdella
Development Genes and Evolution, 1999Cell death was characterized during stages 8 and 9 in the leech Helobdella with a modified terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method. Using confocal analysis, the positions of dying cells were compared to rows of cells expressing the leech engrailed protein ht-en and to fluorescently marked cell lineages.
R, Tsubokawa, C J, Wedeen
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Developmental interdeterminacy in embryos of the leech Helobdella triserialis
Developmental Biology, 1984In embryonic development of the leech Helobdella triserialis, each of the four paired positionally identifiable, ectodermal teloblasts (N, O, P, and Q) generates a bandlet of blast cell progeny that merges with ipsilateral bandlets into a germinal band.
D A, Weisblat, S S, Blair
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Helobdella (Leech): A Model for Developmental Studies
Cold Spring Harbor Protocols, 2009INTRODUCTIONHelobdella is a genus of freshwater leeches, several species of which have been used for developmental studies since the 1970s. Helobdella embryos have been used for cell-lineage tracing and dye-mediated photoablation, and they have also been very useful for studies in cellular neurobiology.
David A, Weisblat, Dian-Han, Kuo
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Micromere lineages in the glossiphoniid leechHelobdella
Development, 2002In leech embryos, segmental mesoderm and ectoderm arise from teloblasts by lineages that are already relatively well characterized. Here, we present data concerning the early divisions and the definitive fate maps of the micromeres, a group of 25 small cells that arise during the modified spiral cleavage in leech (Helobdella robusta) and contribute to ...
Françoise Z, Huang +4 more
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Embryonic origins of cells in the leech Helobdella triserialis
Developmental Biology, 1984To ascertain the embryonic origins of the cells in various tissues of the leech Helobdella triserialis, horseradish peroxidase (HRP) was injected as a cell lineage tracer into all identified blastomeres of the early embryo in turn, except for a few of the micromeres, and the resulting distribution of HRP-labeled cells was then examined in the late ...
D A, Weisblat, S Y, Kim, G S, Stent
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