Results 71 to 80 of about 6,735,850 (224)

A robust and flexible baculovirus-insect cell system for AAV vector production with improved yield, capsid ratios and potency

Molecular Therapy: Methods & Clinical Development
Manufacturing of adeno-associated viruses (AAV) for gene and cell therapy applications has increased significantly and spurred development of improved mammalian and insect cell-based production systems.
Yoko Marwidi, Hoang-Oanh B. Nguyen, David Santos, Tenzin Wangzor, Sumita Bhardwaj, Gabriel Ernie, Gregg Prawdzik, Garrett Lew, David Shivak, Michael Trias, Jada Padilla, Hung Tran, Kathleen Meyer, Richard Surosky, Alex Michael Ward   +14 more
doaj   +1 more source

A new strategy of using satellite RNA to control viral plant diseases: post‐inoculation with satellite RNA attenuates symptoms derived from pre‐infection with its helper virus

Plant Biotechnology Journal, 2019
Satellite RNA (satRNA) is the supernumerary RNA of some viruses. It typically shares no nucleotide homology with its helper virus and depends on its helper virus for replication and encapsidation (Simon et al. 2004).
Xinran Cao, Shanshan Liu, Chengming Yu, Xiangdong Li, Xuefeng Yuan   +4 more
semanticscholar   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Application of Pseudoinfectious Viruses in Transient Gene Expression in Mammalian Cells: Combining Efficient Expression with Regulatory Compliance

Biomolecules
Transient gene expression (TGE) is commonly employed for protein production, but its reliance on plasmid transfection makes it challenging to scale up.
Gulzat Zauatbayeva, Tolganay Kulatay, Bakytkali Ingirbay, Zhanar Shakhmanova, Viktoriya Keyer, Mikhail Zaripov, Maral Zhumabekova, Alexandr V. Shustov   +7 more
doaj   +1 more source

Insect vector-mediated transmission of plant viruses.

Virology, 2015
The majority of plant-infecting viruses are transmitted to their host plants by vectors. The interactions between viruses and vector vary in duration and specificity but some common themes in vector transmission have emerged: 1) plant viruses encode ...
A. Whitfield, B. Falk, D. Rotenberg
semanticscholar   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Transposase-Mediated Construction of an Integrated Adeno-Associated Virus Type 5 Helper Plasmid

BioTechniques, 2002
Adeno-associated viruses (AAVs) are replication-defective parvoviruses that require helper virus functions for efficient productive replication. The AAVs are currently premier candidates as vectors for human gene therapy applications. In particular, much
Richard H. Smith, Sandra A. Afione, Robert M. Kotin   +2 more
doaj   +1 more source

The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants[OPEN]

The Plant Cell, 2016
Bamboo mosaic virus satellite RNA can move autonomously in a fibrillarin-dependent manner in the absence of its helper virus in Nicotiana benthamiana. RNA trafficking plays pivotal roles in regulating plant development, gene silencing, and adaptation to ...
Chih-Hao Chang, Fu-Chen Hsu, Shu-Chuan Lee, Y. Lo, Jiun-Da Wang, Jane Shaw, M. Taliansky, B. Chang, Y. Hsu, N. Lin   +9 more
semanticscholar   +1 more source

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source